(Circulation. 1999;100:1109-1115.)
© 1999 American Heart Association, Inc.
Basic Science Reports |
Endothelial Dysfunction and Collagen Accumulation
Two Independent Factors for Restenosis and Constrictive Remodeling After Experimental Angioplasty
From Necker University (A.L., E.D., B.B., F.A., M.D., C.G.), INSERM U127 (J.L.S.), and INSERM U141 (B.I.L., C.M.B.), Paris, France.
Correspondence to Antoine Lafont, Cardiology, Hopital Boucicaut, 78, rue de la Convention, Paris 75015, France. E-mail antoine.lafont{at}bcc.ap-hop-paris.fr
Background—Constrictive remodeling plays a prominent role in restenosis after balloon angioplasty, but its regulation remains unclear. Because endothelial dysfunction and changes in extracellular matrix have been reported after angioplasty, this study was designed to simultaneously evaluate endothelial function and collagen and elastin changes after restenosis and arterial remodeling.
Methods and Results—Atherosclerosis was induced in femoral arteries of 22 New Zealand White rabbits by air-desiccation and a high-cholesterol diet. One month later, angioplasty was performed. Histomorphometry and in vitro assessment of endothelial function were performed 4 weeks after angioplasty. Restenosis correlated with constrictive remodeling (r=0.60, P=0.01) but not with neointimal growth (r=-0.06, P=0.79). Restenosis correlated with an impaired relaxation to acetylcholine (ACh; r=0.61, P=0.02) but not with the response to the endothelium-independent vasodilator sodium nitroprusside (r=-0.25, P=0.40). Restenosis correlated positively with collagen accumulation (r=0.69, P=0.004) and inversely with elastin density (r=-0.48, P=0.05). Relaxations to ACh were significantly more decreased in arteries with constrictive remodeling than in those with enlargement remodeling (3.7±7.9% versus 35.5±15.0%, P=0.04). Neointimal collagen density was significantly higher in arteries with constrictive remodeling than in those with enlargement remodeling (34.5±4.5% versus 18.2±4.7%, P=0.03). Endothelial function and collagen and elastin density were independent predictors of restenosis in the study.
Conclusions—These results demonstrate that the severity of restenosis after angioplasty correlated with both defective endothelium-dependent relaxation and increased collagen density.
Key Words: angioplasty • restenosis • remodeling • endothelium • collagen
This article has been cited by other articles:
 |
|
 |
|
  B. J. Wu, N. Di Girolamo, K. Beck, C. G. Hanratty, K. Choy, J. Y. Hou, M. R. Ward, and R. Stocker Probucol [4,4'-[(1-Methylethylidene)bis(thio)]bis-[2,6-bis(1,1-dimethylethyl)phenol]] Inhibits Compensatory Remodeling and Promotes Lumen Loss Associated with Atherosclerosis in Apolipoprotein E-Deficient Mice J. Pharmacol. Exp. Ther., May 1, 2007; 321(2): 477 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Menshikov, O. Plekhanova, H. Cai, K. Chalupsky, Y. Parfyonova, P. Bashtrikov, V. Tkachuk, and B. C. Berk Urokinase Plasminogen Activator Stimulates Vascular Smooth Muscle Cell Proliferation Via Redox-Dependent Pathways Arterioscler Thromb Vasc Biol, April 1, 2006; 26(4): 801 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Brasselet, E. Durand, F. Addad, A. A. H. Zen, M. B. Smeets, D. Laurent-Maquin, S. Bouthors, G. Bellon, D. de Kleijn, G. Godeau, et al. Collagen and elastin cross-linking: a mechanism of constrictive remodeling after arterial injury Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2228 - H2233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nakayama, M. Amano, A. Katsumi, T. Kaneko, S. Kawabata, M. Takefuji, and K. Kaibuchi Rho-kinase and myosin II activities are required for cell type and environment specific migration Genes Cells, February 1, 2005; 10(2): 107 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Patti, V. Pasceri, R. Melfi, C. Goffredo, M. Chello, A. D'Ambrosio, R. Montesanti, and G. Di Sciascio Impaired Flow-Mediated Dilation and Risk of Restenosis in Patients Undergoing Coronary Stent Implantation Circulation, January 4, 2005; 111(1): 70 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Baronas-Lowell, J. L. Lauer-Fields, and G. B. Fields Induction of Endothelial Cell Activation by a Triple Helical {alpha}2{beta}1 Integrin Ligand, Derived from Type I Collagen {alpha}1(I)496-507 J. Biol. Chem., January 9, 2004; 279(2): 952 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. F. Leite, A. Danilovic, P. Moriel, K. Dantas, S. Marklund, A. P. V. Dantas, and F. R.M. Laurindo Sustained Decrease in Superoxide Dismutase Activity Underlies Constrictive Remodeling After Balloon Injury in Rabbits Arterioscler Thromb Vasc Biol, December 1, 2003; 23(12): 2197 - 2202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. McLeod, D. E. Newby, D. B. Northridge, K. A.A. Fox, and N. G. Uren Influence of differential vascular remodeling on the coronary vasomotor response Cardiovasc Res, August 1, 2003; 59(2): 520 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N Cheema, N. Nili, C. W Li, H. A Whittingham, J. Linde, R. J van Suylen, M. R Eskandarian, A. P Wong, B. Qiang, J.-F. Tanguay, et al. Effects of intravascular cryotherapy on vessel wall repair in a balloon-injured rabbit iliac artery model Cardiovasc Res, July 1, 2003; 59(1): 222 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J Sierevogel, E. Velema, F. J van der Meer, M. O. Nijhuis, M. Smeets, D. P.V de Kleijn, C. Borst, and G. Pasterkamp Matrix metalloproteinase inhibition reduces adventitial thickening and collagen accumulation following balloon dilation Cardiovasc Res, September 1, 2002; 55(4): 864 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Durand, Z. Mallat, F. Addad, F. Vilde, M. Desnos, C. Guerot, A. Tedgui, and A. Lafont Time courses of apoptosis and cell proliferation and their relationship to arterial remodeling and restenosis after angioplasty in an atherosclerotic rabbit model J. Am. Coll. Cardiol., May 15, 2002; 39(10): 1680 - 1685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. S. Galis and J. J. Khatri Matrix Metalloproteinases in Vascular Remodeling and Atherogenesis: The Good, the Bad, and the Ugly Circ. Res., February 22, 2002; 90(3): 251 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Chandrasekar, S. Nattel, and J.-F. Tanguay Coronary artery endothelial protection after local delivery of 17{beta}-estradiol during balloon angioplasty in a porcine model: a potential new pharmacologic approach to improve endothelial function J. Am. Coll. Cardiol., November 1, 2001; 38(5): 1570 - 1576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Ward, P. Kanellakis, D. Ramsey, J. Funder, and A. Bobik Eplerenone Suppresses Constrictive Remodeling and Collagen Accumulation After Angioplasty in Porcine Coronary Arteries Circulation, July 24, 2001; 104(4): 467 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takano, K. Mizuno, K. Okamatsu, S. Yokoyama, T. Ohba, and S. Sakai Mechanical and structural characteristics of vulnerable plaques: analysis by coronary angioscopy and intravascular ultrasound J. Am. Coll. Cardiol., July 1, 2001; 38(1): 99 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shimokawa, K. Morishige, K. Miyata, T. Kandabashi, Y. Eto, I. Ikegaki, T. Asano, K. Kaibuchi, and A. Takeshita Long-term inhibition of Rho-kinase induces a regression of arteriosclerotic coronary lesions in a porcine model in vivo Cardiovasc Res, July 1, 2001; 51(1): 169 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Morishige, H. Shimokawa, Y. Eto, T. Kandabashi, K. Miyata, Y. Matsumoto, M. Hoshijima, K. Kaibuchi, and A. Takeshita Adenovirus-Mediated Transfer of Dominant-Negative Rho-Kinase Induces a Regression of Coronary Arteriosclerosis in Pigs In Vivo Arterioscler Thromb Vasc Biol, April 1, 2001; 21(4): 548 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-G. Bienvenu, J.-F. Tanguay, J.-F. Theoret, A. Kumar, R. G. Schaub, and Y. Merhi Recombinant Soluble P-Selectin Glycoprotein Ligand-1-Ig Reduces Restenosis Through Inhibition of Platelet-Neutrophil Adhesion After Double Angioplasty in Swine Circulation, February 27, 2001; 103(8): 1128 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shimokawa, K. Morishige, and A. Takeshita C-type natriuretic peptide and vascular remodeling: Reply J. Am. Coll. Cardiol., January 1, 2001; 37(1): 333 - 334. [Full Text] [PDF]
|
|
|
|
|
|
G. Pasterkamp, D. P.V de Kleijn, and C. Borst Arterial remodeling in atherosclerosis, restenosis and after alteration of blood flow: potential mechanisms and clinical implications Cardiovasc Res, March 1, 2000; 45(4): 843 - 852. [Abstract] [Full Text] [PDF]
|
|