Published online before print February 11, 2002, doi: 10.1161/hc1002.105228
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Circulation. 2002;105:1233.)
© 2002 American Heart Association, Inc.
Basic Science Reports |
Local Delivery of Plasmid DNA Into Rat Carotid Artery Using Ultrasound
From the Department of Geriatric Medicine (Y.T., K.H., T.N., K.Y., N.H., M.A., T.O., R.M.) and Division of Gene Therapy Science (Y.T., K.Y., R.M.), Osaka University, Graduate School of Medicine, Osaka, Japan; and the First Department of Anatomy (K.T.), Fukuoka University, School of Medicine, Hakata, Japan.
Correspondence to Ryuichi Morishita, Division of Gene Therapy Science, Osaka University Medical School, 2-2 Yamada-oka, Suita 565, Japan. E-mail morishit{at}geriat.med.osaka-u.ac.jp
Background— Although viral vector systems are efficient to transfect foreign genes into blood vessels, safety issues remain in relation to human gene therapy. In this study, we examined the feasibility of a novel nonviral vector system by using high-frequency, low-intensity ultrasound irradiation for transfection into blood vessels.
Methods and Results— Luciferase plasmid mixed with or without echo contrast microbubble (Optison) was transfected into cultured human vascular smooth muscle cells (VSMC) and endothelial cells (EC) with the use of ultrasound. Interestingly, luciferase activity was markedly increased in both cell types treated with Optison. We then transfected luciferase plasmid mixed with Optison by means of therapeutic ultrasound into rat artery. Two days after transfection, luciferase activity was significantly higher in carotid artery transfected with luciferase gene with Optison and ultrasound than with plasmid alone. In addition, we transfected an anti-oncogene (p53) plasmid into carotid artery after balloon injury as a model of gene therapy for restenosis. Two weeks after transfection, the intimal-to-medial area ratio in rats transfected with wild-type p53 plasmid complexed with Optison by means of ultrasound was significantly decreased as compared with control, accompanied by a significant increase in p53 protein. No apparent toxicity such as inflammation could be detected in blood vessels transfected with plasmid DNA with ultrasound and Optison.
Conclusions— Overall, we demonstrated that an ultrasound transfection method with Optison enhanced transfection efficiency of naked plasmid DNA into blood vessels without any apparent toxicity. Transfection of p53 plasmid with the use of this method should be useful for safe clinical gene therapy without a viral vector system.
Key Words: gene therapy • restenosis • ultrasonics • vessels
This article has been cited by other articles:
 |
|
 |
|
  R. Chen, M. Chiba, S. Mori, M. Fukumoto, and T. Kodama Periodontal Gene Transfer by Ultrasound and Nano/Microbubbles Journal of Dental Research, November 1, 2009; 88(11): 1008 - 1013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. D.M. Meijering, L. J.M. Juffermans, A. van Wamel, R. H. Henning, I. S. Zuhorn, M. Emmer, A. M.G. Versteilen, W. J. Paulus, W. H. van Gilst, K. Kooiman, et al. Ultrasound and Microbubble-Targeted Delivery of Macromolecules Is Regulated by Induction of Endocytosis and Pore Formation Circ. Res., March 13, 2009; 104(5): 679 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, R. J. Singh, K. Usa, B. C. Netzel, and M. Liang Renal medullary 11{beta}-hydroxysteroid dehydrogenase type 1 in Dahl salt-sensitive hypertension Physiol Genomics, December 12, 2008; 36(1): 52 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jin, F. Yi, F. Zhang, J. L. Poklis, and P.-L. Li Lysosomal Targeting and Trafficking of Acid Sphingomyelinase to Lipid Raft Platforms in Coronary Endothelial Cells Arterioscler Thromb Vasc Biol, November 1, 2008; 28(11): 2056 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Marmottant, T. Biben, and S. Hilgenfeldt Deformation and rupture of lipid vesicles in the strong shear flow generated by ultrasound-driven microbubbles Proc R Soc A, July 8, 2008; 464(2095): 1781 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Chappell, J. Song, A. L. Klibanov, and R. J. Price Ultrasonic Microbubble Destruction Stimulates Therapeutic Arteriogenesis Via the CD18-Dependent Recruitment of Bone Marrow-Derived Cells Arterioscler Thromb Vasc Biol, June 1, 2008; 28(6): 1117 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, Z. Wang, H. Ran, X. Li, Q. Yuan, Y. Zheng, J. Ren, L. Su, W. Zhang, Q. Li, et al. Experimental Research on Therapeutic Angiogenesis Induced by Hepatocyte Growth Factor Directed by Ultrasound-Targeted Microbubble Destruction in Rats J. Ultrasound Med., March 1, 2008; 27(3): 453 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Dubinsky, C. Cuevas, M. K. Dighe, O. Kolokythas, and J. H. Hwang High-Intensity Focused Ultrasound: Current Potential and Oncologic Applications Am. J. Roentgenol., January 1, 2008; 190(1): 191 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Duvshani-Eshet, O. Benny, A. Morgenstern, and M. Machluf Therapeutic ultrasound facilitates antiangiogenic gene delivery and inhibits prostate tumor growth Mol. Cancer Ther., August 1, 2007; 6(8): 2371 - 2382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Zhang, G. Zhang, A. Y. Zhang, M. J. Koeberl, E. Wallander, and P.-L. Li Production of NAADP and its role in Ca2+ mobilization associated with lysosomes in coronary arterial myocytes Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H274 - H282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sonoda, K. Tachibana, E. Uchino, A. Okubo, M. Yamamoto, K. Sakoda, T. Hisatomi, K.-H. Sonoda, Y. Negishi, Y. Izumi, et al. Gene Transfer to Corneal Epithelium and Keratocytes Mediated by Ultrasound with Microbubbles Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 558 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Imada, T. Tatsumi, Y. Mori, T. Nishiue, M. Yoshida, H. Masaki, M. Okigaki, H. Kojima, Y. Nozawa, Y. Nishiwaki, et al. Targeted Delivery of Bone Marrow Mononuclear Cells by Ultrasound Destruction of Microbubbles Induces Both Angiogenesis and Arteriogenesis Response Arterioscler Thromb Vasc Biol, October 1, 2005; 25(10): 2128 - 2134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chapman, J. Windle, F. Xie, A. McGrain, and T. R. Porter Incidence of Cardiac Arrhythmias With Therapeutic Versus Diagnostic Ultrasound and Intravenous Microbubbles J. Ultrasound Med., August 1, 2005; 24(8): 1099 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Dean Nonviral gene transfer to skeletal, smooth, and cardiac muscle in living animals Am J Physiol Cell Physiol, August 1, 2005; 289(2): C233 - C245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hauff, S. Seemann, R. Reszka, M. Schultze-Mosgau, M. Reinhardt, T. Buzasi, T. Plath, S. Rosewicz, and M. Schirner Evaluation of Gas-filled Microparticles and Sonoporation as Gene Delivery System: Feasibility Study in Rodent Tumor Models Radiology, August 1, 2005; 236(2): 572 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Dittmar, J. Xie, F. Hunter, C. Trimble, M. Bur, V. Frenkel, and K. C. P. Li Pulsed High-Intensity Focused Ultrasound Enhances Systemic Administration of Naked DNA in Squamous Cell Carcinoma Model: Initial Experience Radiology, May 1, 2005; 235(2): 541 - 546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Morishita, M. Aoki, and T. Ogihara Does gene therapy become pharmacotherapy? Exp Physiol, May 1, 2005; 90(3): 307 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Bekeredjian, P. A. Grayburn, and R. V. Shohet Use of ultrasound contrast agents for gene or drug delivery in cardiovascular medicine J. Am. Coll. Cardiol., February 1, 2005; 45(3): 329 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Song, P. S. Cottler, A. L. Klibanov, S. Kaul, and R. J. Price Microvascular remodeling and accelerated hyperemia blood flow restoration in arterially occluded skeletal muscle exposed to ultrasonic microbubble destruction Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2754 - H2761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Zderic, J. I. Clark, and S. Vaezy Drug Delivery Into the Eye With the Use of Ultrasound J. Ultrasound Med., October 1, 2004; 23(10): 1349 - 1359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kondo, K. Ohmori, A. Oshita, H. Takeuchi, S. Fuke, K. Shinomiya, T. Noma, T. Namba, and M. Kohno Treatment of acute myocardial infarction by hepatocyte growth factor gene transfer: The first demonstration of myocardial transfer of a "functional" gene using ultrasonic microbubble destruction J. Am. Coll. Cardiol., August 4, 2004; 44(3): 644 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wessely, M. Paschalidis, S. Wagenpfeil, F. Wegener, F.-J. Neumann, and W. Theiss A comprehensive approach to visual and functional assessment of experimental vascular lesions in vivo Am J Physiol Heart Circ Physiol, June 1, 2004; 286(6): H2461 - H2467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hiraoka, H. Koike, S. Yamamoto, N. Tomita, C. Yokoyama, T. Tanabe, T. Aikou, T. Ogihara, Y. Kaneda, and R. Morishita Enhanced Therapeutic Angiogenesis by Cotransfection of Prostacyclin Synthase Gene or Optimization of Intramuscular Injection of Naked Plasmid DNA Circulation, November 25, 2003; 108(21): 2689 - 2696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Li, K. Tachibana, M. Kuroki, and M. Kuroki Gene Transfer with Echo-enhanced Contrast Agents: Comparison between Albunex, Optison, and Levovist in Mice--Initial Results Radiology, November 1, 2003; 229(2): 423 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Yang Imaging of Vascular Gene Therapy Radiology, July 1, 2003; 228(1): 36 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Y. Lan, W. Mu, N. Tomita, X. R. Huang, J. H. Li, H.-J. Zhu, R. Morishita, and R. J. Johnson Inhibition of Renal Fibrosis by Gene Transfer of Inducible Smad7 Using Ultrasound-Microbubble System in Rat UUO Model J. Am. Soc. Nephrol., June 1, 2003; 14(6): 1535 - 1548. [Abstract] [Full Text] [PDF]
|
|