Published online before print February 16, 2004, doi: 10.1161/01.CIR.0000118495.88442.32
(Circulation. 2004;109:1161-1167.)
© 2004 American Heart Association, Inc.
Basic Science Reports |
Simultaneous Evaluation of Infarct Size and Cardiac Function in Intact Mice by Contrast-Enhanced Cardiac Magnetic Resonance Imaging Reveals Contractile Dysfunction in Noninfarcted Regions Early After Myocardial Infarction
From the Departments of Biomedical Engineering (Z.Y., S.S.B., W.D.G., M.-C.T., B.A.F.) and Radiology (S.S.B., B.A.F.), University of Virginia Health System, Charlottesville, Va.
Correspondence to Brent A. French, PhD, Biomedical Engineering Department, MR5 Building, Room 1219, University of Virginia, Box 800759, Charlottesville, VA 22903. E-mail bf4g{at}virginia.edu
Received December 23, 2002; de novo received July 22, 2003; revision received October 24, 2003; accepted October 30, 2003.
Background— The objective of this study was to noninvasively determine the effects of reperfused myocardial infarction (MI) on regional and global left-ventricular (LV) function 24 hours after MI in intact mice with contrast-enhanced cardiac MRI and a single, gradient-echo pulse sequence.
Methods and Results— Twenty-three mice received baseline MRI scans followed by either 60 minutes of coronary occlusion (MI group, n=15) or thoracotomy without occlusion (sham group, n=8). Gadolinium-DTPA–enhanced magnetic resonance (MR) images were acquired 24 hours after surgery. Hearts were then excised for conventional infarct size determination via 2,3,5-triphenyl tetrazolium chloride (TTC) staining. In addition to infarct size, analysis of the MR images yielded left ventricular (LV) mass, LV end-systolic volume (LVESV), LV end-diastolic volume (LVEDV), LV ejection fraction (LVEF), cardiac output, and percent LV wall thickening (%WTh). Twenty-four hours after surgery, infarct size was 28.1±1.8% of LV mass by MRI and 27.5±1.7% by TTC (P=NS). Bland-Altman analysis revealed close agreement between the results obtained by the 2 methods. MI had little effect on LVEDV but caused a 98% increase in LVESV (from 11.3 to 22.4 µL, P<0.05), which resulted in a significant reduction in LVEF (from 70% to 37%, P<0.05). Compared with LV regional function at baseline, %WTh 24 hours after MI was significantly depressed, not only in infarcted myocardium but also in regions remote from the infarct zone. In contrast, sham-operated mice showed a small but significant increase in %WTh 24 hours after surgery (P<0.05).
Conclusions— MRI can accurately assess both infarct size and cardiac function in intact mice early after large, reperfused MI, revealing the existence of contractile dysfunction in noninfarcted regions of the heart.
Key Words: myocardial infarction • magnetic resonance imaging • cardiac output • cardiac volume • myocardial contraction
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