Circulation. 2005;112:1451-1461
Published online before print August 29, 2005, doi: 10.1161/CIRCULATIONAHA.105.537480
Published online before print August 29, 2005, doi: 10.1161/CIRCULATIONAHA.105.537480
(Circulation. 2005;112:1451-1461.)
© 2005 American Heart Association, Inc.
Imaging |
Dynamic Imaging of Allogeneic Mesenchymal Stem Cells Trafficking to Myocardial Infarction
From the Russell H. Morgan Department of Radiology and Radiological Science (D.L.K., M.T., W.D.G., T.I., D.K., P.W., W.P.S., H.H.C., D.F., I.I., M.S., B.M.W.T., R.L.W., J.W.M.B.) and Institute for Cell Engineering (D.K., P.W., J.W.M.B.), Johns Hopkins University School of Medicine, Baltimore, Md; Osiris Therapeutics, Inc, Baltimore, Md (R.G.Y., M.M., M.F.P.); and School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pa (R.C.B.).
Correspondence to Dr Dara L. Kraitchman, Johns Hopkins University, 601 N Caroline St, JHOC 4231, Baltimore, MD 21287. E-mail dara{at}mri.jhu.edu
Received January 26, 2005; revision received May 24, 2005; accepted May 31, 2005.
Background— Recent results from animal studies suggest that stem cells may be able to home to sites of myocardial injury to assist in tissue regeneration. However, the histological interpretation of postmortem tissue, on which many of these studies are based, has recently been widely debated.
Methods and Results— With the use of the high sensitivity of a combined single-photon emission CT (SPECT)/CT scanner, the in vivo trafficking of allogeneic mesenchymal stem cells (MSCs) colabeled with a radiotracer and MR contrast agent to acute myocardial infarction was dynamically determined. Redistribution of the labeled MSCs after intravenous injection from initial localization in the lungs to nontarget organs such as the liver, kidney, and spleen was observed within 24 to 48 hours after injection. Focal and diffuse uptake of MSCs in the infarcted myocardium was already visible in SPECT/CT images in the first 24 hours after injection and persisted until 7 days after injection and was validated by tissue counts of radioactivity. In contrast, MRI was unable to demonstrate targeted cardiac localization of MSCs in part because of the lower sensitivity of MRI.
Conclusions— Noninvasive radionuclide imaging is well suited to dynamically track the biodistribution and trafficking of mesenchymal stem cells to both target and nontarget organs.
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