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(Circulation. 2007;115:2022-2032.)
© 2007 American Heart Association, Inc.

Molecular Cardiology

Protective Role of Endogenous Erythropoietin System in Nonhematopoietic Cells Against Pressure Overload–Induced Left Ventricular Dysfunction in Mice

Yasuhide Asaumi, MD; Yutaka Kagaya, MD; Morihiko Takeda, MD; Nobuhiro Yamaguchi, MD; Hiroko Tada, MD; Kenta Ito, MD; Jun Ohta, MD; Takashi Shiroto, MD; Kunio Shirato, MD; Naoko Minegishi, MD; Hiroaki Shimokawa, MD

From the Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine (Y.A., Y.K., M.T., N.Y., H.T., K.I., J.O., T.S., K.S., H.S.), and Biomedical Engineering Research Organization (N.M.), Tohoku University, Sendai, and Japan Science and Technology Agency, CREST, Tokyo (H.S.), Japan.

Correspondence to Yutaka Kagaya, MD, Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, 1–1 Seiryo-Machi, Aoba-Ku, Sendai 980–8574, Japan. E-mail kagaya{at}cardio.med.tohoku.ac.jp

Received August 17, 2006; accepted February 16, 2007.

Background— Erythropoietin (Epo) receptors (EpoRs) are expressed in the heart. We have recently demonstrated that the endogenous Epo-EpoR system plays an important protective role in myocardial ischemia in mice and humans. In the present study, we tested our hypothesis that the endogenous Epo-EpoR system in nonhematopoietic cells also plays a protective role against pressure overload–induced cardiac dysfunction in vivo.

Methods and Results— Transgene-rescued EpoR-null mutant mice (EpoR^–/–_rescued) that express EpoR exclusively in the hematopoietic cells were subjected to transverse aortic constriction (TAC). At 1 week after TAC, left ventricular weight and lung weight were significantly increased in EpoR^–/–_rescued mice compared with wild-type mice, although the fibrotic area was comparably increased after TAC in the 2 genotypes. In the EpoR^–/–_rescued mice with TAC, left ventricular end-diastolic diameter was significantly increased, left ventricular fractional shortening was significantly decreased, and survival rate was significantly decreased compared with wild-type mice with TAC. Phosphorylation of STAT3 at 5 hours and 1 week after TAC and that of p38 at 5 hours after TAC were significantly increased in wild-type mice but not in EpoR^–/–_rescued mice. Vascular endothelial growth factor protein expression and capillary density in left ventricular myocardium were significantly decreased in EpoR^–/–_rescued mice with TAC compared with wild-type mice with TAC.

Conclusions— These results suggest that the endogenous Epo-EpoR system in the nonhematopoietic cells plays an important protective role against pressure overload–induced cardiac dysfunction in vivo.

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