Circulation, Vol 71, 1247-1254, Copyright © 1985 by American Heart Association
C Kishimoto, K Kuribayashi, T Masuda, N Tomioka and C Kawai
To clarify the immune mechanism in myocarditis, we examined by
immunofluorescence techniques the serial changes in percentages of T and B
lymphocytes in the heart, spleen, and peripheral blood of DBA/2 mice
inoculated with encephalomyocarditis (EMC) virus (experiment I). B cells
were demonstrated by staining with fluorescein isothiocyanate
(FITC)-labeled rabbit antimouse immunoglobulin (Ig). T cells were
demonstrated with rat anti-Thy1.2 monoclonal antibody plus FITC-labeled
antimouse Ig. There was a marked decrease in T cells in peripheral blood
and a moderate decrease in the cells in the spleen on day 14. There were no
significant changes in B cells in peripheral blood or spleen throughout the
entire period and T cells accounted for approximately 80% of the cells in
the myocardium on days 7 and 14. To confirm the involvement of T cells in
the development of myocarditis, we also carried out studies in which
BALB/c-nu/nu mice (group 1, n = 58), BALB/c-nu/+ mice (group 2, n = 54),
and BALB/c-nu/nu mice injected with 5 X 10(7) spleen cells from BALB/c-nu/+
mice (group 3, n = 50) were inoculated with EMC virus (experiment II). Four
mice from each of the three groups were killed on day 6 for virologic
studies. In experiment II, there were no significant differences in the
incidence of myocarditis among the three groups. Virus titrations of the
heart and serum neutralizing antibody titers did not show any significant
differences between the three groups on days 6 and 16.(ABSTRACT TRUNCATED
AT 250 WORDS)
ARTICLES
Immunologic behavior of lymphocytes in experimental viral myocarditis: significance of T lymphocytes in the severity of myocarditis and silent myocarditis in BALB/c-nu/nu mice
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