Circulation, Vol 73, 1065-1076, Copyright © 1986 by American Heart Association
KP Gallagher, AJ Buda, D Pace, RA Gerren and M Shlafer
Superoxide dismutase (SOD) and catalase (CAT), enzymes that degrade
superoxide anion and hydrogen peroxide, respectively, reduce size of
infarction in anesthetized, open-chest dogs subjected to coronary occlusion
followed by reperfusion. To evaluate potential protective effects of these
enzymes in conscious animals, three groups of dogs were instrumented at
sterile surgery with a hydraulic occluder on the left circumflex (LCX)
coronary artery, sonomicrometers to measure regional wall thickness, and
catheters to monitor arterial and left ventricular pressures. Ten to 14
days after surgery, the animals were sedated with morphine sulfate (0.5
mg/kg). The LCX artery was occluded for 3 hr by inflation of the hydraulic
cuff. Infusions of SOD (n = 7), CAT (n = 6), or saline (control group, n =
7) were begun 15 min before reperfusion and lasted for 45 min of
reperfusion. The doses of SOD and CAT were 5 mg/kg, dissolved in 60 ml of
saline, and infused at a rate of 1 ml/min. Myocardial blood flow was
measured with tracer-labeled microspheres (15 micron diameter) before
occlusion, after 5 to 10 min of occlusion, after 150 min of occlusion, and
5 to 10 min after reperfusion. Size of infarction was measured 24 hr later
by dual- perfusion staining with Evans blue and triphenyl tetrazolium. Size
of infarction (expressed as a percentage of area at risk) did not differ
significantly among the three groups: control, 32 +/- 17% (mean +/- SD);
SOD, 38 +/- 17%; CAT, 27 +/- 17%. Hemodynamic parameters and myocardial
blood flows (measured before infusion of any agents) were not significantly
different among the three groups. Serum SOD levels in SOD-treated dogs were
19 +/- 2 micrograms/ml at the onset of reperfusion and 29 +/- 3
micrograms/ml at the end of the infusion. Blood assays collected after
infusion showed a monoexponential decay of SOD levels with a half-life of
22 +/- 6 min. We conclude that myocardial protection by SOD or CAT is model
dependent. In conscious dogs subjected to 3 hr of coronary occlusion
followed by reperfusion, SOD and CAT failed to alter size of infarction.
ARTICLES
Failure of superoxide dismutase and catalase to alter size of infarction in conscious dogs after 3 hours of occlusion followed by reperfusion
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