Circulation, Vol 75, 1083-1090, Copyright © 1987 by American Heart Association
P Chatelain, JG Latour, D Tran, M de Lorgeril, G Dupras and M Bourassa
The effects of reperfusion on the myocardial accumulation of neutrophils
and their role in the extent of injury were investigated in a canine
preparation with a 3 hr coronary occlusion followed by 21 hr of
reperfusion. The left anterior descending coronary artery (LAD) was
permanently occluded in group 1 and reperfused after 3 hr in four others
(groups 2 to 5). All but group 5 received lidocaine (1 mg/min over 8 hr). A
critical stenosis was produced and left in place at reperfusion only in
group 2. In groups 1 and 2, 111In-labeled autologous neutrophils were
injected at the time of coronary occlusion. Group 4 animals were rendered
leukopenic 2 hr before the coronary ligature and throughout the experiment
by injection of an antineutrophil rabbit serum. Quantification of the
radioactivity by digitized scintigraphy of the heart slices revealed an 80%
(p less than .05) increase in neutrophil accumulation in the infarct region
after reperfusion (group 2) as compared with permanent occlusion (group 1).
Gamma counting of myocardial tissue samples showed that the neutrophil
accumulation ratio in the subendocardial central zone of the infarct was
increased five times (p less than .05) by reperfusion, whereas no
difference was evident in the subepicardium. Infarct size and myocardial
area at risk were not statistically different among the five groups.
However LAD flow in the leukopenic group (group 4) was significantly higher
(p less than .05) 30 min after reperfusion (40.0 +/- 5 ml/min) when
compared with the preocclusion value (21.7 +/- 4 ml/min). In contrast, in a
parallel experiment without leukopenia (group 3), LAD flow after
reperfusion did not differ from the preocclusion value.(ABSTRACT TRUNCATED
AT 250 WORDS)
ARTICLES
Neutrophil accumulation in experimental myocardial infarcts: relation with extent of injury and effect of reperfusion
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