Circulation, Vol 81, 1594-1605, Copyright © 1990 by American Heart Association
JJ Armbrecht, DB Buxton and HR Schelbert
Extraction and clearance kinetics of [1-11C]acetate were examined in 65
experiments in 30 open-chest dogs. Twenty-nine studies were performed at
control, 13 during ischemia, eight after reperfusion, 13 during
dipyridamole-induced hyperemia, and two during alteration of cardiac
workload. [1-11C]Acetate was injected directly into the left anterior
descending coronary artery, and myocardial tissue-time activity curves were
recorded with a gamma probe. The single-pass extraction fraction averaged
64.2 +/- 9.7% in control, 65.3 +/- 9.1% in ischemia, 70.0 +/- 4.4% in
reperfusion, and 46.5 +/- 7.4% in dipyridamole-induced hyperemia groups.
11C clearance was biexponential in all cases. The rate constant k1 for the
first rapid clearance phase correlated closely with myocardial oxygen
consumption (r = 0.94) in control, ischemia, reperfusion, and
dipyridamole-induced hyperemia groups. Monoexponential fitting of only the
first linear part of the clearance curve yielded the rate constant kmono,
which also correlated with myocardial oxygen consumption (r = 0.96).
Arterial lactate concentrations and the amount of free fatty acid oxygen
equivalents consumed by the myocardium were shown to have a small but
statistically significant impact on the relation between [1-11C]acetate
clearance rate constants and myocardial oxygen consumption. The fraction of
14CO2 activity contributing to overall 14C activity leaving the myocardium
after simultaneous injection of [1-14C]acetate (n = 24) was relatively high
in all cases (97.4 +/- 2.5% in control, 89 +/- 2.6% in ischemia, 94.1 +/-
3.5% in reperfusion, and greater than 99% in dipyridamole groups),
indicating that externally measured 11C clearance corresponds to CO2
production and thus to tricarboxylic acid cycle activity. In conclusion,
the results validate the use of [1-11C]acetate as a tracer of oxidative
myocardial metabolism for use with positron emission tomography.
ARTICLES
Validation of [1-11C]acetate as a tracer for noninvasive assessment of oxidative metabolism with positron emission tomography in normal, ischemic, postischemic, and hyperemic canine myocardium
Department of Radiological Sciences, UCLA School of Medicine 90024.
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