Circulation, Vol 82, 586-594, Copyright © 1990 by American Heart Association
JE Quillen, FW Sellke, LA Brooks and DG Harrison
We examined the effects of ischemia with and without reperfusion on
endothelium-dependent and -independent vascular relaxation in both conduit
and resistance coronary arteries. Studies were performed on dogs under
control conditions (n = 13) or after 1 hour of circumflex coronary artery
occlusion with (n = 10) or without (n = 8) 1 hour of reperfusion. Rings of
obtuse marginal branches of the left circumflex coronary artery (conduit
arteries) were studied in organ chambers. Coronary microvessels
(110-220-microns diameter) were studied in a pressurized state with an in
vitro microvessel imaging apparatus. Relaxation was evaluated after
preconstriction with prostaglandin F2 alpha and U46619 (a thromboxane A2
analogue) in conduit and resistance vessels, respectively. Conduit vessel
function was not altered by ischemia with or without reperfusion.
Endothelium-dependent microvascular relaxation was depressed in response to
acetylcholine, ADP, and calcium ionophore A23187 after ischemia with
reperfusion compared with control relaxation (ED50 as -log[M]: 6.0 +/- 0.2
[p less than 0.05], 5.1 +/- 0.4 [p less than 0.05], and 5.8 +/- 0.1 versus
6.8 +/- 0.2, 6.8 +/- 0.2, and 6.6 +/- 0.2, respectively). Ischemia without
reperfusion modestly altered microvascular endothelium-dependent
relaxation. Microvascular relaxation to nitroglycerin was not altered by
ischemia with reperfusion. We conclude that 1) endothelium-dependent
relaxation in large epicardial coronary arteries is relatively refractory
to ischemia with or without reperfusion, 2) ischemia alone produces mild
alterations of coronary microvascular reactivity, 3) ischemia followed by
reperfusion produces a marked and selective impairment of
endothelium-dependent responses in the coronary microcirculation.
ARTICLES
Ischemia-reperfusion impairs endothelium-dependent relaxation of coronary microvessels but does not affect large arteries
University of Iowa, Cardiovascular Center, Iowa City.
This article has been cited by other articles:
 |
|
 |
|
  M. Kambe, R. Bessho, M. Fujii, M. Ochi, and K. Shimizu Sivelestat reduces myocardial ischemia and reperfusion injury in rat hearts even when administered after onset of myocardial ischemia Interactive CardioVascular and Thoracic Surgery, June 1, 2009; 8(6): 629 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lopez, A. Rodriguez-Sinovas, L. Agullo, J. Inserte, A. Cabestrero, and D. Garcia-Dorado Acidic reoxygenation protects against endothelial dysfunction in rat aortic rings submitted to simulated ischemia Am J Physiol Heart Circ Physiol, December 1, 2008; 295(6): H2409 - H2416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Feng, C. Bianchi, J. Li, and F. W. Sellke Bradykinin Preconditioning Preserves Coronary Microvascular Reactivity During Cardioplegia-Reperfusion Ann. Thorac. Surg., March 1, 2005; 79(3): 911 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Ensunsa, J. D. Symons, L. Lanoue, H. R. Schrader, and C. L. Keen Reducing Arginase Activity via Dietary Manganese Deficiency Enhances Endothelium-Dependent Vasorelaxation of Rat Aorta Experimental Biology and Medicine, December 1, 2004; 229(11): 1143 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Symons, Y. Hayashi, and J. L. Ensunsa Improved coronary vascular function evoked by high-intensity treadmill training is maintained in arteries exposed to ischemia and reperfusion J Appl Physiol, October 1, 2003; 95(4): 1638 - 1647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J Flood, L. Willems, and J. P Headrick Coronary function and adenosine receptor-mediated responses in ischemic-reperfused mouse heart Cardiovasc Res, July 1, 2002; 55(1): 161 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Charkoudian, J. H. Eisenach, J. L. D. Atkinson, R. D. Fealey, and M. J. Joyner Effects of chronic sympathectomy on locally mediated cutaneous vasodilation in humans J Appl Physiol, February 1, 2002; 92(2): 685 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Goodwin, R. T. Smolenski, C. C. Gray, J. Jayakumar, M. Amrani, and M. H. Yacoub Role of endogenous endothelin on coronary reflow after cardioplegic arrest J. Thorac. Cardiovasc. Surg., December 1, 2001; 122(6): 1167 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Symons and S. Schaefer Na+/H+ exchange subtype 1 inhibition reduces endothelial dysfunction in vessels from stunned myocardium Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1575 - H1582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takehana, G. A. Beller, M. Ruiz, F. D. Petruzella, D. D. Watson, and D. K. Glover Assessment of Residual Coronary Stenoses Using 99mTc-N-NOET Vasodilator Stress Imaging to Evaluate Coronary Flow Reserve Early After Coronary Reperfusion in a Canine Model of Subendocardial Infarction J. Nucl. Med., September 1, 2001; 42(9): 1388 - 1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Chen, S.-C. Wu, C.-H. Huang, P.-C. Pan, C.-S. Lee, and C.-C. Lin Morphometric identification of luminal narrowing of myocardial capillaries after cardioplegic arrest Ann. Thorac. Surg., January 1, 2001; 71(1): 243 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hayashida, S. Chihara, E. Tayama, S. Yokose, K. Akasu, E. Kai, and S. Aoyagi Effects of angiotensin-converting enzyme inhibitor during warm blood cardioplegia Ann. Thorac. Surg., August 1, 2000; 70(2): 627 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yasuda, Y. Goto, T. Baba, T. Satoh, H. Sumida, S. Miyazaki, and H. Nonogi Enhanced secretion of cardiac hepatocyte growth factor from an infarct region is associated with less severe ventricular enlargement and improved cardiac function J. Am. Coll. Cardiol., July 1, 2000; 36(1): 115 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Symons, S. V. Rendig, C. L. Stebbins, and J. C. Longhurst Microvascular and myocardial contractile responses to ischemia: influence of exercise training J Appl Physiol, February 1, 2000; 88(2): 433 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Saitoh, M. Hashimoto, K. Ku, S. Kin, S. Nosaka, S. Masumura, and K. Nakayama Heart preservation in HTK solution: role of coronary vasculature in recovery of cardiac function Ann. Thorac. Surg., January 1, 2000; 69(1): 107 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Liu Constriction to hypoxia-reoxygenation in isolated mouse coronary arteries: role of endothelium and superoxide J Appl Physiol, October 1, 1999; 87(4): 1392 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. L. Serebruany, V. V. Yurovsky, and P. A. Gurbel Mild Myocardial Stunning Affects Platelet Aggregation and Certain Hemostatic Factors in Swine Clinical and Applied Thrombosis/Hemostasis, October 1, 1999; 5(4): 236 - 242. [Abstract] [PDF]
|
|
|
|
|
|
S. M. Wildhirt, S. Weismueller, C. Schulze, N. Conrad, A. Kornberg, and B. Reichart Inducible nitric oxide synthase activation after ischemia/reperfusion contributes to myocardial dysfunction and extent of infarct size in rabbits: evidence for a late phase of nitric oxide-mediated reperfusion injury Cardiovasc Res, August 15, 1999; 43(3): 698 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Park, M. Tofukuji, C. Metais, M. E. Comunale, H. B. Dai, M. Simons, G. L. Stahl, A. Agah, and F. W. Sellke Attenuation of Endothelium-Dependent Dilation of Pig Pulmonary Arterioles After Cardiopulmonary Bypass Is Prevented by Monoclonal Antibody to Complement C5a Anesth. Analg., July 1, 1999; 89(1): 42 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Marrelli, A. Khorovets, T. D. Johnson, W. F. Childres, and R. M. Bryan Jr. P2 purinoceptor-mediated dilations in the rat middle cerebral artery after ischemia-reperfusion Am J Physiol Heart Circ Physiol, January 1, 1999; 276(1): H33 - H41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Huang, M. Wu, C. Meininger, K. Kelly, and Y. Yuan Neutrophil-dependent augmentation of PAF-induced vasoconstriction and albumin flux in coronary arterioles Am J Physiol Heart Circ Physiol, October 1, 1998; 275(4): H1138 - H1147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. O. Cannon III Role of nitric oxide in cardiovascular disease: focus on the endothelium Clin. Chem., August 1, 1998; 44(8): 1809 - 1819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bouchard, M.-A. Despatis, J. Buluran, and R. Cartier Vascular Effects of Cyclosporin A and Acute Rejection in Canine Heart Transplantation Ann. Thorac. Surg., November 1, 1997; 64(5): 1325 - 1330. [Abstract] [Full Text]
|
|
|
|
|
|
H. Sato, J. E. Jordan, Z.-Q. Zhao, S. S. Sarvotham, and J. Vinten-Johansen Gradual Reperfusion Reduces Infarct Size and Endothelial Injury but Augments Neutrophil Accumulation Ann. Thorac. Surg., October 1, 1997; 64(4): 1099 - 1107. [Abstract] [Full Text]
|
|
|
|
|
|
M. S. Bayfield, J. R. Lindner, S. Kaul, S. Ismail, M. L. K. Sheil, N. C. Goodman, R. Zacour, and W. D. Spotnitz DEOXYGENATED BLOOD MINIMIZES ADHERENCE OF SONICATED ALBUMIN MICROBUBBLES DURING CARDIOPLEGIC ARREST AND AFTER BLOOD REPERFUSION: EXPERIMENTAL AND CLINICAL OBSERVATIONS WITH MYOCARDIAL CONTRAST ECHOCARDIOGRAPHY J. Thorac. Cardiovasc. Surg., June 1, 1997; 113(6): 1100 - 1108. [Abstract] [Full Text]
|
|
|
|
|
|
C. O. Murphy, Pan-Chih, J. P. Gott, and R. A. Guyton Coronary Microvascular Reactivity After Ischemic Cold Storage and Reperfusion Ann. Thorac. Surg., January 1, 1997; 63(1): 20 - 27. [Abstract] [Full Text]
|
|
|
|
|
|
F. E. Schmidt Jr, M. J. MacDonald, C. O. Murphy, W. M. Brown III, J. P. Gott, and R. A. Guyton Leukocyte Depletion of Blood Cardioplegia Attenuates Reperfusion Injury Ann. Thorac. Surg., December 1, 1996; 62(6): 1691 - 1696. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. P. Tiefenbacher, W. M. Chilian, M. Mitchell, and D. V. DeFily Restoration of Endothelium-Dependent Vasodilation After Reperfusion Injury by Tetrahydrobiopterin Circulation, September 15, 1996; 94(6): 1423 - 1429. [Abstract] [Full Text]
|
|
|
|
|
|
R. N. Piana, S. Y. Wang, M. Friedman, and F. W. Sellke Angiotensin-Converting Enzyme Inhibition Preserves Endothelium-Dependent Coronary Microvascular Responses During Short-term Ischemia-Reperfusion Circulation, February 1, 1996; 93(3): 544 - 551. [Abstract] [Full Text]
|
|
|
|
|
|
C. O. Murphy, Pan-Chih, J. P. Gott, and R. A. Guyton Microvascular Reactivity After Crystalloid, Cold Blood, and Warm Blood Cardioplegic Arrest Ann. Thorac. Surg., October 1, 1995; 60(4): 1021 - 1027. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. A. Aiello, R. I. Jabr, and W. C. Cole Arrhythmia and Delayed Recovery of Cardiac Action Potential During Reperfusion After Ischemia : Role Of Oxygen Radical–Induced No-Reflow Phenomenon Circ. Res., July 1, 1995; 77(1): 153 - 162. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kadletz, R. J. Dignan, K. E. Loesser, M. L. Hess, and A. S. Wechsler Ischemia and activated neutrophils alter coronary microvascular but not epicardial coronary artery reactivity J. Thorac. Cardiovasc. Surg., October 1, 1994; 108(4): 648 - 657. [Abstract] [Full Text]
|
|
|
|
|
|
F. X. McGowan Jr., H. Cao-Danh, K. Takeuchi, P. J. Davis, and P. J. del Nido Prolonged neonatal myocardial preservation with a highly buffered low-calcium solution J. Thorac. Cardiovasc. Surg., October 1, 1994; 108(4): 772 - 779. [Abstract] [Full Text]
|
|
|
|
|
|
C. Saldanha and D. J. Hearse Cardioplegia and vascular injuryDissociation of the effects of ischemia from those of the cardioplegic solution J. Thorac. Cardiovasc. Surg., August 1, 1994; 108(2): 279 - 290. [Abstract] [Full Text]
|
|