Circulation, Vol 83, 402-411, Copyright © 1991 by American Heart Association
DD Ku, JB Caulfield and JK Kirklin
In the present study, responses of long-term human coronary artery bypass
grafts (CABGs) to known endothelium-dependent vasodilators, acetylcholine,
calcium ionophore A23187, thrombin, and histamine, as well as authentic
nitric oxide, the putative endothelium-derived relaxing factor, were
studied. Sixteen CAGBs were isolated within 1-2 hours from hearts of 14
patients receiving a cardiac transplant. A total of 109 ring segments were
prepared from these CABGs and studied in vitro. The duration of the CABGs
ranged from 7 months to 12 years. Addition of acetylcholine (0.01-10
microM), calcium ionophore A23187 (0.01-1.0 microM), thrombin (0.01-1.0
unit/ml), and histamine (0.01-1.0 microM) consistently produced a dose- and
endothelium-dependent relaxation, reaching a maximum of -35.3 +/- 3.3%,
-45.3 +/- 5.5%, -26.9 +/- 4.8%, and -17.8 +/- 2.5% (mean +/- SEM),
respectively. No significant difference was observed among the CABGs with
different duration of transplantation, whereas the relaxant responses of
different segments along the entire length of a CABG were markedly
different. These latter differences in the endothelium-dependent responses
appear to correlate inversely with the development of intimal proliferative
lesions in these CABGs. Addition of nitric oxide (0.01-10 microM) produced
a potent dose- and endothelium-independent relaxation, which was also
slightly depressed in CABGs with severe intimal proliferation. These
results demonstrate that long-term transplanted human saphenous vein grafts
retain their endothelium-dependent responses and that development of severe
intimal proliferative lesions, rather than the duration of the grafts,
result in marked alterations in the reactivity of these transplanted CABGs.
ARTICLES
Endothelium-dependent responses in long-term human coronary artery bypass grafts
Department of Pharmacology, University of Alabama, Birmingham 35294.
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