Circulation, Vol 84, 2539-2546, Copyright © 1991 by American Heart Association
S Takeda, GB Pier, Y Kojima, M Tojo, E Muller, T Tosteson and DA Goldmann
BACKGROUND. Staphylococcus epidermidis is the principal pathogen in
prosthetic valve endocarditis. The capsular polysaccharide adhesin (PS/A)
has been shown to mediate attachment of bacteria to medical devices. In
this study, we investigated the efficacy of active and passive immunization
against PS/A in preventing S. epidermidis endocarditis in a rabbit model.
METHODS AND RESULTS. Aortic valve vegetations were produced by inserting a
Teflon catheter into the left ventricle through the right carotid artery.
Bacteremia and endocarditis were then established by implanting in the left
jugular vein a catheter that was attached to an osmotic pump and
contaminated with S. epidermidis strain RP62A. During a 3-week study
period, of 64 blood cultures taken every second or third day from six
nonimmune rabbits, 54 (84%) yielded strain RP62A. In rabbits actively
immunized with PS/A, eight of 60 blood cultures (13%) were positive (odds
ratio 5.0, 95% CI, 2.0-12.3, p = 0.005). At death, all six nonimmune
rabbits had infected vegetations that yielded 10(6)-10(11) colony-forming
units (cfu)/g of vegetation, whereas only one PS/A-immunized rabbit had an
infected vegetation. Immunization protocols designed to elicit antibody to
teichoic acid but not to PS/A afforded no protection against bacteremia or
endocarditis. Infusion of monoclonal antibody to PS/A through a catheter in
the right jugular vein provided a level of protection against both
bacteremia and endocarditis comparable to that produced by active
immunization. In vitro, antibody against PS/A was opsonic for S.
epidermidis. CONCLUSIONS. Immunoprophylaxis targeted at staphylococcal PS/A
is a promising new approach to the prevention of prosthetic valve
endocarditis.
ARTICLES
Protection against endocarditis due to Staphylococcus epidermidis by immunization with capsular polysaccharide/adhesin
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital.
This article has been cited by other articles:
 |
|
 |
|
  B. R. Sellman, Y. Timofeyeva, J. Nanra, A. Scott, J. P. Fulginiti, Y. V. Matsuka, and S. M. Baker Expression of Staphylococcus epidermidis SdrG Increases following Exposure to an In Vivo Environment Infect. Immun., July 1, 2008; 76(7): 2950 - 2957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cerca, T. Maira-Litran, K. K. Jefferson, M. Grout, D. A. Goldmann, and G. B. Pier Protection against Escherichia coli infection by antibody to the Staphylococcus aureus poly-N-acetylglucosamine surface polysaccharide PNAS, May 1, 2007; 104(18): 7528 - 7533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Theilacker, Z. Kaczynski, A. Kropec, F. Fabretti, T. Sange, O. Holst, and J. Huebner Opsonic Antibodies to Enterococcus faecalis Strain 12030 Are Directed against Lipoteichoic Acid. Infect. Immun., October 1, 2006; 74(10): 5703 - 5712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cerca, K. K. Jefferson, R. Oliveira, G. B. Pier, and J. Azeredo Comparative Antibody-Mediated Phagocytosis of Staphylococcus epidermidis Cells Grown in a Biofilm or in the Planktonic State. Infect. Immun., August 1, 2006; 74(8): 4849 - 4855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Sellman, A. P. Howell, C. Kelly-Boyd, and S. M. Baker Identification of Immunogenic and Serum Binding Proteins of Staphylococcus epidermidis Infect. Immun., October 1, 2005; 73(10): 6591 - 6600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Maira-Litran, A. Kropec, D. A. Goldmann, and G. B. Pier Comparative Opsonic and Protective Activities of Staphylococcus aureus Conjugate Vaccines Containing Native or Deacetylated Staphylococcal Poly-N-Acetyl-{beta}-(1-6)-Glucosamine Infect. Immun., October 1, 2005; 73(10): 6752 - 6762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Maira-Litran, A. Kropec, C. Abeygunawardana, J. Joyce, G. Mark III, D. A. Goldmann, and G. B. Pier Immunochemical Properties of the Staphylococcal Poly-N-Acetylglucosamine Surface Polysaccharide Infect. Immun., August 1, 2002; 70(8): 4433 - 4440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Cramton, M. Ulrich, F. Gotz, and G. Doring Anaerobic Conditions Induce Expression of Polysaccharide Intercellular Adhesin in Staphylococcus aureus and Staphylococcus epidermidis Infect. Immun., June 1, 2001; 69(6): 4079 - 4085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. McCormick, H. Hirt, C. M. Waters, T. J. Tripp, G. M. Dunny, and P. M. Schlievert Antibodies to a Surface-Exposed, N-terminal Domain of Aggregation Substance Are Not Protective in the Rabbit Model of Enterococcus faecalis Infective Endocarditis Infect. Immun., May 1, 2001; 69(5): 3305 - 3314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. McKenney, K. L. Pouliot, Y. Wang, V. Murthy, M. Ulrich, G. Döring, J. C. Lee, D. A. Goldmann, and G. B. Pier Broadly Protective Vaccine for Staphylococcus aureus Based on an in Vivo-Expressed Antigen Science, May 28, 1999; 284(5419): 1523 - 1527. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Rupp, J. S. Ulphani, P. D. Fey, K. Bartscht, and D. Mack Characterization of the Importance of Polysaccharide Intercellular Adhesin/Hemagglutinin of Staphylococcus epidermidis in the Pathogenesis of Biomaterial-Based Infection in a Mouse Foreign Body Infection Model Infect. Immun., May 1, 1999; 67(5): 2627 - 2632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shiro, G. Meluleni, A. Groll, E. Muller, T. D. Tosteson, D. A. Goldmann, and G. B. Pier The Pathogenic Role of Staphylococcus epidermidis Capsular Polysaccharide/Adhesin in a Low-Inoculum Rabbit Model of Prosthetic Valve Endocarditis Circulation, November 1, 1995; 92(9): 2715 - 2722. [Abstract] [Full Text]
|
|