Circulation, Vol 86, 1977-1986, Copyright © 1992 by American Heart Association
A Rothman, TJ Kulik, MB Taubman, BC Berk, CW Smith and B Nadal-Ginard
BACKGROUND. Pulmonary hypertension is associated with abnormal pulmonary
arterial contractility and growth. The mechanisms for these abnormalities
are largely unknown. To study these processes, we sought to develop an in
vitro system. Even though cultured aortic and pulmonary artery smooth
muscle cells (SMCs) have been of considerable value in studying smooth
muscle biology, one drawback of this system has been that these cells often
lose differentiated properties in an unpredictable manner when they are
passaged in culture. In addition, there appear to be significant
differences in physiological and pathological responses between the
systemic and pulmonary circulations, many of which could be directly
related to the smooth muscle. We therefore established a cloned population
of rat pulmonary arterial SMCs (PASMCs) that maintain differentiated
properties through multiple subcultures. METHODS AND RESULTS. PASMCs were
obtained initially by enzymatic dissociation from pulmonary arteries of
adult Sprague-Dawley rats. From these cells, clones were isolated. The
cloned cells retained expression of functional surface receptors for
angiotensin II, norepinephrine, and alpha-thrombin and high levels of the
smooth muscle isoforms of alpha-actin, myosin heavy chain, myosin
regulatory light chain, and alpha-tropomyosin mRNAs even after multiple
passages. The cells could also be transfected and processed exogenous
transcripts in a smooth muscle-specific fashion. CONCLUSIONS. These cloned
PASMCs retain many differentiated characteristics and should be valuable
for future studies of pulmonary vascular smooth muscle cell biology.
ARTICLES
Development and characterization of a cloned rat pulmonary arterial smooth muscle cell line that maintains differentiated properties through multiple subcultures
Department of Pediatrics, University of California, San Diego.
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