Circulation, Vol 87, 208-215, Copyright © 1993 by American Heart Association
M Kitakaze, M Hori, S Takashima, H Sato, M Inoue and T Kamada
BACKGROUND. Adenosine has been reported to mediate the necrosis- limiting
effects of ischemic preconditioning; however, it is unclear how this
mediation occurs. The present study was undertaken to test the hypothesis
that ischemic preconditioning increases 5'-nucleotidase activity and
adenosine release during sustained ischemia and subsequent reperfusion.
METHODS AND RESULTS. After thoracotomy, the left anterior descending
coronary artery was cannulated and perfused with blood redirected from the
left carotid artery in 32 dogs. Ischemic preconditioning was produced by
four cycles in which the coronary artery was occluded and then reperfused
for 5 minutes each. After the last cycle of ischemia and reperfusion, the
coronary artery was occluded for 40 minutes. This was followed by 120
minutes of reperfusion. In the control group, the coronary artery was
occluded for 40 minutes and reperfused for 120 minutes without ischemic
preconditioning. The plasma adenosine concentration was measured and blood
gases were analyzed in coronary arterial and venous blood samples taken
during 120 minutes of reperfusion. Myocardial 5'-nucleotidase activity was
measured before and at 40 minutes of sustained ischemia with and without
ischemic preconditioning. The adenosine concentration in coronary venous
blood during reperfusion was significantly higher in preconditioned hearts
than in the control hearts: 1 minute after the onset of reperfusion, 546
+/- 57 versus 244 +/- 41 pmol/ml; 10 minutes after, 308 +/- 30 versus 114
+/- 14 pmol/ml; 30 minutes after, 175 +/- 24 versus 82 +/- 16 pmol/ml,
respectively (p < 0.01). Ectosolic and cytosolic 5'-nucleotidase
activities increased in both endocardial and epicardial myocardium in the
ischemia-preconditioned hearts. Furthermore, 40 minutes of ischemia
increased 5'-nucleotidase activity in ischemia-preconditioned hearts more
than in control hearts. CONCLUSIONS. Ischemic preconditioning increases
adenosine release and 5'-nucleotidase activity during sustained ischemia
and subsequent reperfusion.
ARTICLES
Ischemic preconditioning increases adenosine release and 5'- nucleotidase activity during myocardial ischemia and reperfusion in dogs. Implications for myocardial salvage [published erratum appears in Circulation 1993 May;87(5):1775 and 1993 Jun;87(6):2070]
First Department of Medicine, Osaka University School of Medicine, Japan.
This article has been cited by other articles:
 |
|
 |
|
  S. Rane, M. He, D. Sayed, H. Vashistha, A. Malhotra, J. Sadoshima, D. E. Vatner, S. F. Vatner, and M. Abdellatif Downregulation of MiR-199a Derepresses Hypoxia-Inducible Factor-1{alpha} and Sirtuin 1 and Recapitulates Hypoxia Preconditioning in Cardiac Myocytes Circ. Res., April 10, 2009; 104(7): 879 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Headrick, B. Hack, and K. J. Ashton Acute adenosinergic cardioprotection in ischemic-reperfused hearts Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H1797 - H1818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rochetaing and P. Kreher Reactive hyperemia during early reperfusion as a determinant of improved functional recovery in ischemic preconditioned rat hearts J. Thorac. Cardiovasc. Surg., June 1, 2003; 125(6): 1516 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Matsusaka, N. Hasebe, Y.-T. Jin, J. Kawabe, and K. Kikuchi Magnesium reduces myocardial infarct size via enhancement of adenosine mechanism in rabbits Cardiovasc Res, June 1, 2002; 54(3): 568 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kitakaze, K. Node, H. Asanuma, S. Takashima, Y. Sakata, M. Asakura, S. Sanada, Y. Shinozaki, H. Mori, T. Kuzuya, et al. Protein Tyrosine Kinase Is Not Involved in the Infarct Size-Limiting Effect of Ischemic Preconditioning in Canine Hearts Circ. Res., August 18, 2000; 87(4): 303 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sakaguchi, Y. Sawa, M. Kitakaze, K. Suzuki, M. Nishimura, Y. Kaneda, and H. Matsuda Ecto-5'-nucleotidase plays a role in the cardioprotective effects of heat shock protein 72 in ischemia-reperfusion injury in rat hearts Cardiovasc Res, July 1, 2000; 47(1): 74 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Belhomme, J. Peynet, M. Louzy, J.-M. Launay, M. Kitakaze, and P. Menasche Evidence for Preconditioning by Isoflurane in Coronary Artery Bypass Graft Surgery Circulation, November 9, 1999; 100 (2009): II-340 - II-344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A Gustafson, C. J Zuurbier, J. E Bassett, J. P. F Barends, J. H.G.M van Beek, J. B Bassingthwaighte, and K. Kroll Increased hypoxic stress decreases AMP hydrolysis in rabbit heart Cardiovasc Res, November 1, 1999; 44(2): 333 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takeo and Y. Nasa Role of energy metabolism in the preconditioned heart - a possible contribution of mitochondria Cardiovasc Res, July 1, 1999; 43(1): 32 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miki, T. Miura, R. Bunger, K. Suzuki, J. Sakamoto, and K. Shimamoto Ecto-5'-nucleotidase is not required for ischemic preconditioning in rabbit myocardium in situ Am J Physiol Heart Circ Physiol, October 1, 1998; 275(4): H1329 - H1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Harrison, R. J. Willis, and J. P. Headrick Extracellular adenosine levels and cellular energy metabolism in ischemically preconditioned rat heart Cardiovasc Res, October 1, 1998; 40(1): 74 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takasaki, N. Adachi, K. Dote, S. Tsubota, T. Yorozuya, and T. Arai Ischemic preconditioning suppresses the noradrenaline turnover in the rat heart Cardiovasc Res, August 1, 1998; 39(2): 373 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Kloner, R. Bolli, E. Marban, L. Reinlib, and E. Braunwald Medical and Cellular Implications of Stunning, Hibernation, and Preconditioning : An NHLBI Workshop Circulation, May 19, 1998; 97(18): 1848 - 1867. [Full Text] [PDF]
|
|
|
|
|
|
L. A. Gustafson and K. Kroll Downregulation of 5'-nucleotidase in rabbit heart during coronary underperfusion Am J Physiol Heart Circ Physiol, February 1, 1998; 274(2): H529 - H538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Minamino, M. Kitakaze, H. Sato, H. Funaya, Y. Ueda, H. Asanuma, T. Kuzuya, and M. Hori Effects of ischemic preconditioning on contractile and metabolic function during hypoperfusion in dogs Am J Physiol Heart Circ Physiol, February 1, 1998; 274(2): H684 - H693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Giannella, H.-C. Mochmann, and R. Levi Ischemic Preconditioning Prevents the Impairment of Hypoxic Coronary Vasodilatation Caused by Ischemia/Reperfusion : Role of Adenosine A1/A3 and Bradykinin B2 Receptor Activation Circ. Res., September 19, 1997; 81(3): 415 - 422. [Abstract] [Full Text]
|
|
|
|
|
|
R. Nawada, T. Murakami, T. Iwase, K. Nagai, Y. Morita, I. Kouchi, M. Akao, and S. Sasayama Inhibition of Sarcolemmal Na+,K+-ATPase Activity Reduces the Infarct Size–Limiting Effect of Preconditioning in Rabbit Hearts Circulation, July 15, 1997; 96(2): 599 - 604. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. L. Capecchi, F. L. Pasini, G. Cati, M. Colafati, A. Acciavatti, L. Ceccatelli, S. Petri, A. de Lalla, T. Di Perri, and P. L. Capecchi Experimental Model of Short-Time Exercise-Induced Preconditioning in POAD Patients Angiology, June 1, 1997; 48(6): 469 - 480. [Abstract] [PDF]
|
|
|
|
|
|
R. S. Vander Heide and J. D. Marsh Through the Looking Glass: A Positive Inotropic Drug and Ischemic Preconditioning Circulation, April 15, 1997; 95(8): 1989 - 1991. [Full Text]
|
|
|
|
|
|
M. Kitakaze, T. Minamino, H. Funaya, K. Node, Y. Shinozaki, H. Mori, and M. Hori Vesnarinone Limits Infarct Size via Adenosine-Dependent Mechanisms in the Canine Heart Circulation, April 15, 1997; 95(8): 2108 - 2114. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Miyamae, I. Diamond, M. W. Weiner, S. A. Camacho, and V. M. Figueredo Regular alcohol consumption mimics cardiac preconditioning by protecting against ischemia-reperfusion injury PNAS, April 1, 1997; 94(7): 3235 - 3239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Engler and D. M. Yellon Sulfonylurea KATP Blockade in Type II Diabetes and Preconditioning in Cardiovascular Disease: Time for Reconsideration Circulation, November 1, 1996; 94(9): 2297 - 2301. [Full Text]
|
|
|
|
|
|
D. W. Stepp, R. Van Bibber, K. Kroll, and E. O. Feigl Quantitative Relation Between Interstitial Adenosine Concentration and Coronary Blood Flow Circ. Res., September 1, 1996; 79(3): 601 - 610. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kitakaze, K. Node, T. Minamino, K. Komamura, H. Funaya, Y. Shinozaki, M. Chujo, H. Mori, M. Inoue, M. Hori, et al. Role of Activation of Protein Kinase C in the Infarct SizeLimiting Effect of Ischemic Preconditioning Through Activation of Ecto-5'-nucleotidase Circulation, February 15, 1996; 93(4): 781 - 791. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Minamino, M. Kitakaze, K. Komamura, K. Node, H. Takeda, M. Inoue, M. Hori, and T. Kamada Activation of Protein Kinase C Increases Adenosine Production in the Hypoxic Canine Coronary Artery Through the Extracellular Pathway Arterioscler Thromb Vasc Biol, December 1, 1995; 15(12): 2298 - 2304. [Abstract] [Full Text]
|
|
|
|
|
|
T. Mizumura, K. Nithipatikom, and G. J. Gross Bimakalim, an ATP-Sensitive Potassium Channel Opener, Mimics the Effects of Ischemic Preconditioning to Reduce Infarct Size, Adenosine Release, and Neutrophil Function in Dogs Circulation, September 1, 1995; 92(5): 1236 - 1245. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. A. Bukhari, I. B. Krukenkamp, P. G. Burns, G. R. Gaudette, J. J. Schulman, M. R. Al-Fagih, and S. Levitsky Does Aprotinin Increase the Myocardial Damage in the Setting of Ischemia and Preconditioning? Ann. Thorac. Surg., August 1, 1995; 60(2): 307 - 310. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kitakaze, M. Hori, T. Morioka, T. Minamino, S. Takashima, Y. Okazaki, K. Node, K. Komamura, K. Iwakura, T. Itoh, et al. {alpha}1-Adrenoceptor Activation Increases Ecto-5'-Nucleotidase Activity and Adenosine Release in Rat Cardiomyocytes by Activating Protein Kinase C Circulation, April 15, 1995; 91(8): 2226 - 2234. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. R. Meldrum, M. B. Mitchell, A. Banerjee, and A. H. Harken Cardiac Preconditioning: Induction of Endogenous Tolerance to Ischemia-Reperfusion Injury Arch Surg, November 1, 1993; 128(11): 1208 - 1211. [Abstract] [PDF]
|
|
|
|
|
|
H. Ninomiya, H. Otani, K. Lu, T. Uchiyama, M. Kido, and H. Imamura Complementary role of extracellular ATP and adenosine in ischemic preconditioning in the rat heart Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1810 - H1820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ninomiya, H. Otani, K. Lu, T. Uchiyama, M. Kido, and H. Imamura Enhanced IPC by activation of pertussis toxin-sensitive and -insensitive G protein-coupled purinoceptors Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1933 - H1943. [Abstract] [Full Text] [PDF]
|
|