Circulation, Vol 89, 1922-1928, Copyright © 1994 by American Heart Association
SJ Chen, JM Wilson and DW Muller
BACKGROUND: The efficacy of aorto-coronary vein grafting is limited by
early graft thrombosis and accelerated graft atherosclerosis. Direct
adenovirus-mediated transfer of genes encoding inhibitory proteins may
prevent or slow progression of vein graft disease. METHODS AND RESULTS:
Recombinant adenoviruses containing the cDNA for the marker gene lacZ
(Ad.CMVlacZ) or soluble vascular cell adhesion molecule (sVCAM) (Ad.CB-
sVCAM) were used to infect segments of porcine jugular vein or human
saphenous vein. Ex vivo testing showed expression of the introduced genes
after incubation with Ad.CMVlacZ or Ad.CBsVCAM for periods from 1 to 24
hours, with an increase in transfection efficiency with increasing
incubation time. Porcine jugular veins were then interposed as vascular
grafts in the carotid arteries of four juvenile farm pigs after ex vivo
gene transfer by incubation for 90 to 120 minutes with Ad.CMVlacZ or
Ad.CBsVCAM. sVCAM-transfected carotid vein grafts were placed on one side
and lacZ transfected veins were placed contralaterally as controls. Three
days later, the vein graft segments were resected. Expression of the lacZ
gene was confirmed by X-Gal chromagen staining and visualization by light
and transmission electron microscopy. Gene expression was apparent in all
layers of the vein graft wall, with prominent staining in the adventitia.
sVCAM expression was confirmed by immunohistochemistry and in situ
hybridization. CONCLUSIONS: We conclude that ex vivo gene transfer before
vein grafting is feasible using a replication-deficient recombinant
adenovirus and results in a high level of gene expression in vivo. The
potential for this approach to prevent early vein graft thrombosis or
accelerated vein graft atherosclerosis requires further evaluation.
ARTICLES
Adenovirus-mediated gene transfer of soluble vascular cell adhesion molecule to porcine interposition vein grafts
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0119.
This article has been cited by other articles:
 |
|
 |
|
  PREVENT IV Investigators Efficacy and Safety of Edifoligide, an E2F Transcription Factor Decoy, for Prevention of Vein Graft Failure Following Coronary Artery Bypass Graft Surgery: PREVENT IV: A Randomized Controlled Trial JAMA, November 16, 2005; 294(19): 2446 - 2454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Chiu-Pinheiro, T. O'Brien, Z. S. Katusic, L. F. Bonilla, C. E. Hamner, and H. V. Schaff Gene transfer to coronary artery bypass conduits Ann. Thorac. Surg., October 1, 2002; 74(4): 1161 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Abrahams, C. Song, S. DeFelice, M. S. Grady, S. L. Diamond, and R. J. Levy Endovascular Microcoil Gene Delivery Using Immobilized Anti-adenovirus Antibody for Vector Tethering Stroke, May 1, 2002; 33(5): 1376 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.Y. Park, S. Lee, K.H. Park, C.T. Lee, Y.W. Kim, S.K. Han, Y.S. Shim, and C.G. Yoo Anti-inflammatory effect of adenovirus-mediated I{kappa}B{alpha} overexpression in respiratory epithelial cells Eur. Respir. J., November 1, 2001; 18(5): 801 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. J. Dzau, M. J. Mann, A. Ehsan, and D. P. Griese Gene therapy and genomic strategies for cardiovascular surgery: The emerging field of surgiomics J. Thorac. Cardiovasc. Surg., February 1, 2001; 121(2): 0206 - 216. [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Meyerson, C. L. Skelly, M. A. Curi, and L. B. Schwartz Gene Therapy for Cardiovascular Disease Seminars in Cardiothoracic and Vascular Anesthesia, November 1, 2000; 4(4): 289 - 300. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Ehsan and M. J Mann Antisense and gene therapy to prevent restenosis Vascular Medicine, May 1, 2000; 5(2): 103 - 114. [Abstract] [PDF]
|
|
|
|
|
|
M. O Hiltunen, M. P Turunen, M. Laitinen, and S. Yla-Herttuala Insights into the molecular pathogenesis of atherosclerosis and therapeutic strategies using gene transfer Vascular Medicine, February 1, 2000; 5(1): 41 - 48. [Abstract] [PDF]
|
|
|
|
 |
|
 G. Vassalli, R. Agah, R. Qiao, C. Aguilar, and D. A. Dichek A Mouse Model of Arterial Gene Transfer : Antigen-Specific Immunity Is a Minor Determinant of the Early Loss of Adenovirus-Mediated Transgene Expression Circ. Res., October 29, 1999; 85 (9): e25 - e32. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hein, M. Ernst, F. Moller, and D. Regensburger Gene transfer into rat heart-derived endothelial cells Eur. J. Cardiothorac. Surg., April 1, 1999; 13(4): 460 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-z. Bai, Y. Sawa, W.-d. Zhang, T. Yamakawa, R. Morishita, Y. Kaneda, and H. Matsuda Gene transfer to vein graft wall by HVJ-liposome method: time course and localization of gene expression Ann. Thorac. Surg., September 1, 1998; 66(3): 814 - 819. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Davies, T. T. T. Huynh, G. J. Fulton, R. J. Lefkowitz, E. Svendsen, P.-O. Hagen, and W. J. Koch G Protein Signaling and Vein Graft Intimal Hyperplasia : Reduction of Intimal Hyperplasia in Vein Grafts by a Gß{gamma} Inhibitor Suggests a Major Role of G Protein Signaling in Lesion Development Arterioscler Thromb Vasc Biol, August 1, 1998; 18(8): 1275 - 1280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Motwani and E. J. Topol Aortocoronary Saphenous Vein Graft Disease : Pathogenesis, Predisposition, and Prevention Circulation, March 10, 1998; 97(9): 916 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J Feldman and G. Steg Optimal techniques for arterial gene transfer Cardiovasc Res, September 1, 1997; 35(3): 391 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H Baker, D. Mehta, S. J George, and G. D Angelini Prevention of vein graft failure: potential applications for gene therapy Cardiovasc Res, September 1, 1997; 35(3): 442 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M Channon, G. J Fulton, J. L Gray, B. H Annex, G. A Shetty, M. A Blazing, K. G Peters, P.-O. Hagen, and S. E George Efficient adenoviral gene transfer to early venous bypass grafts: comparison with native vessels Cardiovasc Res, September 1, 1997; 35(3): 505 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Davies, B. H. Annex, G. J. Fulton, S. M. Denning, D. A. Taylor, M. A. Blazing, K. G. Peters, K. M. Channon, S. E. George, and P.-O. Hagen Alteration of Arterial Vasomotor Function in Vitro by Gene Transfer with a Replication-Deficient Adenovirus Vascular and Endovascular Surgery, March 1, 1997; 31(2): 131 - 136. [Abstract] [PDF]
|
|
|
|
|
|
R. E. Gerszten, F. W. Luscinskas, H. T. Ding, D. A. Dichek, L. M. Stoolman, M. A. Gimbrone Jr, and A. Rosenzweig Adhesion of Memory Lymphocytes to Vascular Cell Adhesion Molecule-1–Transduced Human Vascular Endothelial Cells Under Simulated Physiological Flow Conditions In Vitro Circ. Res., December 1, 1996; 79(6): 1205 - 1215. [Abstract] [Full Text]
|
|
|
|
|
|
B. L. Robinson, T. Morita, K. Fujita, M. Chow, H. V. Schaff, and J. J. Morris BYPASS CONDUIT VESSEL WALL BIOLOGY SUBSTANTIALLY INFLUENCES DOWNSTREAM MYOCARDIAL CONTRACTILE RESPONSE TO INJURY FROM ISCHEMIA AND REPERFUSION J. Thorac. Cardiovasc. Surg., January 1, 1996; 111(1): 62 - 73. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ueno, J.-J. Li, H. Tomita, H. Yamamoto, Y. Pan, Y. Kanegae, I. Saito, and A. Takeshita Quantitative Analysis of Repeat Adenovirus-Mediated Gene Transfer Into Injured Canine Femoral Arteries Arterioscler Thromb Vasc Biol, December 1, 1995; 15(12): 2246 - 2253. [Abstract] [Full Text]
|
|
|
|
|
|
A. H. Schulick, K. D. Newman, R. Virmani, and D. A. Dichek In Vivo Gene Transfer Into Injured Carotid Arteries : Optimization and Evaluation of Acute Toxicity Circulation, May 1, 1995; 91(9): 2407 - 2414. [Abstract] [Full Text]
|
|
|
|
|
|
E. G. Nabel Gene Therapy for Cardiovascular Disease Circulation, January 15, 1995; 91(2): 541 - 548. [Full Text]
|
|
|
|
|
|
PREVENT IV Investigators Efficacy and Safety of Edifoligide, an E2F Transcription Factor Decoy, for Prevention of Vein Graft Failure Following Coronary Artery Bypass Graft Surgery: PREVENT IV: A Randomized Controlled Trial JAMA, November 16, 2005; 294(19): 2446 - 2454. [Abstract] [Full Text] [PDF]
|
|