Circulation, Vol 90, 2285-2290, Copyright © 1994 by American Heart Association
T Tagawa, T Imaizumi, T Endo, M Shiramoto, Y Harasawa and A Takeshita
BACKGROUND--The role of nitric oxide (NO) in reactive hyperemia (RH) is not
well known. We investigated whether NO plays a role in RH in human forearm
vessels by examining the effects of NG-monomethyl-L-arginine (L- NMMA), a
blocker of NO synthesis, on reactive hyperemic flow. METHODS AND
RESULTS--Forearm blood flow (FBF) was measured by strain-gauge
plethysmography with a venous occlusion technique. The left brachial artery
was cannulated for drug infusion and direct measurement of arterial
pressure. To produce RH, blood flow to the forearm was prevented by
inflation of a cuff on the upper arm to suprasystolic pressure for
intervals of 3 and 10 minutes. After the release of arterial occlusion
(AO), FBF was measured every 15 seconds for 3 minutes. Resting FBF was 4.3
+/- 0.3 mL.min-1.100 mL-1 before 3 minutes of AO and 4.1 +/- 0.6
mL.min-1.100 mL-1 before 10 minutes of AO. FBF increased to 32.3 +/- 1.9
and 38.2 +/- 3.1 mL.min-1.100 mL-1 immediately after 3 and 10 minutes of
AO, respectively, and gradually decayed (n = 13). Intra-arterial infusion
of L-NMMA (4 mumol/min for 5 minutes) decreased baseline FBF (P < .01)
without changes in arterial pressure. L-NMMA did not affect the peak
reactive hyperemic FBF after 3 and 10 minutes of AO. L-NMMA significantly
decreased total reactive hyperemic flow (flow debt repayment) by 20% to 30%
after 3 and 10 minutes of AO. Simultaneous infusion of L-arginine (a
precursor of NO) with L-NMMA reversed the effects of L-NMMA.
CONCLUSIONS--Our results suggest that NO plays a minimal role in
vasodilation at peak RH but plays a modest yet significant role in
maintaining vasodilation after peak vasodilation. Our results also suggest
that reactive hyperemia in human forearms is caused largely by mechanisms
other than NO.
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Role of nitric oxide in reactive hyperemia in human forearm vessels
Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
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