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(Circulation. 1995;92:3397-3400.)
© 1995 American Heart Association, Inc.
Articles |
Chlamydia pneumoniae (TWAR) in Atherosclerosis of the Carotid Artery
From the Departments of Epidemiology (J.T.G.), Pathobiology (J.T.G., C-c.K., L.A.C., S-p.W.), and Surgery (E.D.S.), University of Washington, Seattle; the Dameron Hospital Heart Institute (A.S.C., R.D.L.), Stockton, Calif; and the Harborview Medical Center (M.J.L.), University of Washington, Seattle.
Correspondence to J. Thomas Grayston, MD, Department of Epidemiology, Box 357236, University of Washington, Seattle, WA 98195-7236.
Background Chlamydia pneumoniae has been demonstrated in atherosclerotic lesions of coronary arteries and aorta. A seroepidemiological study found C pneumoniae–specific antibody more frequently in persons with significant carotid artery wall thickening than in matched control subjects.
Methods and Results Fresh-frozen or formalin-fixed tissue obtained at carotid endarterectomy was examined by immunocytochemistry (ICC) and the polymerase chain reaction (PCR) for the presence of C pneumoniae. Five of five fresh-frozen and formalin-fixed carotid endarterectomy specimens were positive for C pneumoniae by ICC (three of five by PCR). A total of 56 archival formalin-fixed, paraffin-embedded carotid endarterectomy tissues from three hospitals were examined by ICC. Thirty-two were positive. Thirteen normal carotid artery tissue sections from six patients were negative for C pneumoniae.
Conclusions C pneumoniae organisms are frequently found in the advanced carotid atherosclerotic lesions of persons undergoing endarterectomy. Although these findings do not establish causality for C pneumoniae in carotid artery atherosclerosis, they should stimulate investigation of a possible causal or pathogenic role for the organism in the disease.
Key Words: immunohistochemistry • polymerase chain reaction • chlamydia • immunology
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