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(Circulation. 1995;92:705-709.)
© 1995 American Heart Association, Inc.

Articles

Osteopontin Expression Is Increased in the Heritable Cardiomyopathy of Syrian Hamsters

Errol B. Williams, MD; Igor Halpert, MD; Samuel Wickline, MD; Glenn Davison, MD; William C. Parks, PhD; Jeffrey N. Rottman, MD

From the Department of Internal Medicine, Divisions of Cardiology and Dermatology (W.C.P.), Washington University School of Medicine, St Louis, Mo.

Correspondence to Jeff Rottman, MD, Cardiology Division, Jewish Hospital at Washington University Medical Center, 216 S Kingshighway, St Louis, MO 63110.

Background Osteopontin, a noncollagenous matrix protein, is transiently expressed in the heart after experimental cardiac injury, but its expression in states of continuing cardiac remodeling is unknown. We evaluated osteopontin expression in the heritable cardiomyopathy of the Syrian hamster.

Methods and Results Hamster hearts were obtained for RNA isolation and analysis and in situ hybridization from two groups: normal control animals (n=4) and untreated cardiomyopathic hamsters (n=5). Osteopontin mRNA was 12-fold greater in cardiomyopathic hearts compared with normal controls (1.76±0.31 versus 0.14±0.04 arbitrary units normalized to GAPDH, mean±SEM, P<.05). In situ hybridization was used to define the origin of osteopontin in the heart. Osteopontin mRNA above background levels was not detected in sections from noncardiomyopathic hamster hearts but was readily detected in sections from cardiomyopathic hamsters, in which it originated in cells morphologically consistent with tissue macrophages.

Conclusions In the hamster, osteopontin is expressed in heritably cardiomyopathic hearts under conditions of chronic injury and repair, and the source of osteopontin message appears to be tissue macrophage-like cells in foci of inflammation. This model could be used to evaluate the biological role of osteopontin in myocardial inflammation and remodeling.

Key Words: osteopontin • proteins • cardiomyopathy • RNA

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