(Circulation. 1996;93:1791-1795.)
© 1996 American Heart Association, Inc.
Articles |
Missense Mutation in the Pore Region of HERG Causes Familial Long QT Syndrome
From the Cardiovascular Division, Brigham and Women's Hospital, Boston, Mass (D.W.B., C.E.S.); the Department of Genetics, Howard Hughes Medical Institute, Boston, Mass (C.A.M., J.G.S.); and the Department of Cardiology, Children's Hospital, Boston, Mass (M.R.V., C.A.S.).
Correspondence to Carol Ann Satler, MD, PhD, Children's Hospital, Department of Cardiology, 300 Longwood Ave, Enders 13, Boston, MA 02115. E-mail satler@phenix.tch.harvard.edu.
Background Long QT syndrome (LQT) is an inherited cardiac disorder that results in syncope, seizures, and sudden death. In a family with LQT, we identified a novel mutation in human ether-a-go-go–related gene (HERG), a voltage-gated potassium channel.
Methods and Results We used DNA sequence analysis, restriction enzyme digestion analysis, and allele-specific oligonucleotide hybridization to identify the HERG mutation. A single nucleotide substitution of thymidine to guanine (T1961G) changed the coding sense of HERG from isoleucine to arginine (Ile593Arg) in the channel pore region. The mutation was present in all affected family members; the mutation was not present in unaffected family members or in 100 normal, unrelated individuals.
Conclusions We conclude that the Ile593Arg missense mutation in HERG is the cause of LQT in this family because it segregates with disease, its presence was confirmed in three ways, and it is not found in normal individuals. The Ile593Arg mutation may result in a change in potassium selectivity and permeability leading to a loss of HERG function, thereby resulting in LQT.
Key Words: tachycardia • syncope • genetics • torsade de pointes
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