This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Doenst, T. Articles by Taegtmeyer, H. Search for Related Content PubMed PubMed Citation Articles by Doenst, T. Articles by Taegtmeyer, H.

(Circulation. 1998;97:2454-2462.)
© 1998 American Heart Association, Inc.

Basic Science Reports

Profound Underestimation of Glucose Uptake by [¹⁸F]2-Deoxy-2-fluoroglucose in Reperfused Rat Heart Muscle

Torsten Doenst, MD; ; Heinrich Taegtmeyer, MD, DPhil

From the Department of Medicine, Division of Cardiology, University of Texas–Houston Medical School (T.D., H.T.), and Department of Cardiovascular Surgery, University of Freiburg, Germany (T.D.).

Correspondence to Heinrich Taegtmeyer, MD, DPhil, Department of Internal Medicine, Division of Cardiology, University of Texas–Houston Medical School, 6431 Fannin, MSB 1.246, Houston, TX 77030. E-mail ht{at}heart.med.uth.tmc.edu

Background—[¹⁸F]2-deoxy-2-fluoroglucose (FDG) is widely used as a tracer for glucose uptake in ischemic heart muscle. We tested the effects of low-flow ischemia and reperfusion on the ratio of tracer/tracee (lumped constant, LC).

Methods and Results—Isolated working rat hearts were perfused with Krebs-Henseleit buffer containing only glucose 5 mmol/L (group 1) or glucose 5 mmol/L plus oleate 0.4 mmol/L (group 2, fed; group 3, fasted). Dynamic glucose uptake was measured simultaneously with [2-³H]glucose and with FDG. After 20 minutes, coronary flow was reduced by 75% for 30 minutes before it was returned to control conditions for the final 20 minutes. Hexokinase activity in the cytosolic and mitochondrial fractions and tissue metabolites were determined. Rates of glucose uptake were highest when glucose was the only substrate. Glucose uptake, FDG uptake, and the LC increased during ischemia only in group 3. There was no change of these parameters during ischemia in groups 1 and 2. FDG uptake decreased significantly with reperfusion in groups 2 and 3, and there was a striking fall in the LC (from >1.0 to <0.2, P<.001). The fall in the LC was associated with a significant increase in intracellular free glucose. Neither ischemia nor reperfusion affected the kinetic properties of hexokinase.

Conclusions—FDG profoundly underestimates glucose uptake during reperfusion in the presence of fatty acids. In the fasted state, however, FDG overestimates glucose uptake during ischemia. The results indicate limitations in the use of FDG to quantify myocardial glucose uptake in human heart.

Key Words: ischemia • radioisotopes • nuclear medicine • enzymes • glucose • fatty acids

This article has been cited by other articles:

				R. Carroll, A. N. Carley, J. R. B. Dyck, and D. L. Severson Metabolic effects of insulin on cardiomyocytes from control and diabetic db/db mouse hearts Am J Physiol Endocrinol Metab, May 1, 2005; 288(5): E900 - E906. [Abstract] [Full Text] [PDF]

				B. P. Cupps, B. J. Pomerantz, M. D. Krock, J. Villard, J. Rogers, N. Moazami, and M. K. Pasque Principal Strain Orientation in the Normal Human Left Ventricle Ann. Thorac. Surg., April 1, 2005; 79(4): 1338 - 1343. [Abstract] [Full Text] [PDF]

				K. Mather, M. Laakso, S. Edelman, G. Hook, and A. Baron Evidence for physiological coupling of insulin-mediated glucose metabolism and limb blood flow Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1264 - E1270. [Abstract] [Full Text] [PDF]

				T. Utriainen, S. Lovisatti, S. Makimattila, A. Bertoldo, S. Weintraub, R. DeFronzo, C. Cobelli, and H. Yki-Jarvinen Direct measurement of the lumped constant for 2-deoxy-[1-14C]glucose in vivo in human skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2000; 279(1): E228 - E233. [Abstract] [Full Text] [PDF]

				J. A. Fallavollita, C. Trojan, and J. M. Canty Jr. Transmural distribution of FDG uptake in stunned myocardium Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H102 - H109. [Abstract] [Full Text] [PDF]

				P. H. McNulty, D. Jagasia, G. W. Cline, C. K. Ng, J. M. Whiting, P. Garg, G. I. Shulman, and R. Soufer Persistent Changes in Myocardial Glucose Metabolism In Vivo During Reperfusion of a Limited-Duration Coronary Occlusion Circulation, February 29, 2000; 101(8): 917 - 922. [Abstract] [Full Text] [PDF]

				K. F. Kofoed, H. Schoder, R. J. Knight, and D. B. Buxton Glucose metabolism in reperfused myocardium measured by [2-18F] 2-fluorodeoxyglucose and PET Cardiovasc Res, January 14, 2000; 45(2): 321 - 329. [Abstract] [Full Text] [PDF]

				R. Malhotra and F. C. Brosius III Glucose Uptake and Glycolysis Reduce Hypoxia-induced Apoptosis in Cultured Neonatal Rat Cardiac Myocytes J. Biol. Chem., April 30, 1999; 274(18): 12567 - 12575. [Abstract] [Full Text] [PDF]

				K. Hashimoto, T. Nishimura, K.-I. Imahashi, H. Yamaguchi, M. Hori, and H. Kusuoka Lumped constant for deoxyglucose is decreased when myocardial glucose uptake is enhanced Am J Physiol Heart Circ Physiol, January 1, 1999; 276(1): H129 - H133. [Abstract] [Full Text] [PDF]