From the Copenhagen Muscle Research Centre, August Krogh Institute, and
Rigshospitalet, University of Copenhagen, Denmark.
Correspondence to Ylva Hellsten, Copenhagen Muscle Research Centre, August Krogh Institute, Universitetsparken 13, DK-2100 Copenhagen Ø, Denmark. E-mail ylva.hellsten{at}pop.aki.ku.dk
Background—Adenosine has
been proposed to be a locally produced regulator of blood flow in
skeletal muscle. However, the fundamental questions of to what extent
adenosine is formed in skeletal muscle tissue of humans,
whether it is present in the interstitium, and where it exerts its
vasodilatory effect remain unanswered.
Methods and Results—The interstitial
adenosine concentration was determined in the vastus lateralis
muscle of healthy humans via dialysis probes inserted in the muscle.
The probes were perfused with buffer, and the dialysate samples were
collected at rest and during graded knee extensor exercise. At rest,
the interstitial concentration of adenosine was
220±100 nmol/L and femoral arterial blood flow (FaBF) was
0.19±0.02 L/min. When the subjects exercised lightly, at a work rate
of 10 W, there was a markedly higher (1140±540 nmol/L;
P<0.05) interstitial adenosine
concentration and a higher FaBF (2.22±0.18 L/min;
P<0.05) compared with at rest. When exercise was
performed at 20, 30, 40, or 50 W, the concentration of
adenosine was moderately greater for each increment, as was the
level of leg blood flow. The interstitial concentrations of
ATP, ADP, and AMP increased from rest (0.13±0.03, 0.07±0.03, and
0.07±0.02 µmol/L, respectively) to exercise (10 W; 2.00±1.32,
2.08±1.23, and 1.65±0.50 µmol/L, respectively;
P<0.05).
Conclusions—The present study provides, for the first time,
interstitial adenosine concentrations in human
skeletal muscle and demonstrates that adenosine and its
precursors increase in the exercising muscle interstitium, at a rate
associated with intensity of muscle contraction and the magnitude of
muscle blood flow.
© 1998 American Heart Association, Inc.
Brief Rapid Communications
Adenosine Concentrations in the Interstitium of Resting and Contracting Human Skeletal Muscle
Key Words: blood flow • exercise • adenosine • muscles • interstitium
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