(Circulation. 2000;101:841.)
© 2000 American Heart Association, Inc.
Brief Rapid Communications |
From the Institut National de la Santé et la Recherche Médicale, INSERM U141, IFR Circulation, Hôpital Lariboisière, Paris (Z.M., A.T.); Service de Cardiologie (H.B., P.G.S.) and Service de Biochimie (J.B.), Hôpital Bichat, Paris; Institut dHématologie et dImmunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg; and INSERM U143, Le Kremlin-Bicêtre (B.H., J.-M.F.), France.
Correspondence to Alain Tedgui, PhD, INSERM U 141, 41 boulevard de la Chapelle, 75475 Paris Cedex 10, France. E-mail tedgui{at}infobiogen.fr
| Abstract |
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Methods and ResultsWe studied 39 patients with coronary heart disease, including 12 patients with stable angina and 27 patients with acute coronary syndromes (ACS), and 12 patients with noncoronary heart disease. We isolated the circulating microparticles by capture with annexin V and determined their procoagulant potential with a prothrombinase assay. The cell origins of microparticles were determined in an additional 22 patients by antigenic capture with specific antibodies. The level of procoagulant microparticles did not differ between stable angina patients and noncoronary patients (10.1±1.6 nmol/L phosphatidylserine [PS] equivalent versus 9.9±1.6 nmol/L PS equivalent, respectively). However, procoagulant microparticles were significantly elevated in patients with ACS (22.2±2.7 nmol/L PS equivalent) compared with other coronary (P<0.01) or noncoronary (P<0.01) patients. Microparticles of endothelial origin were significantly elevated in patients with ACS (P<0.01), which suggests an important role for endothelial injury in inducing the procoagulant potential.
ConclusionsHigh levels of procoagulant endothelial microparticles are present in the circulating blood of patients with ACS and may contribute to the generation and perpetuation of intracoronary thrombi.
Key Words: atherosclerosis complications thrombosis microparticles endothelium.
| Introduction |
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| Methods |
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All coronary patients were receiving aspirin. Patients with ACS received additional standard antithrombotic therapy before blood sampling. Anti-ischemic medications were equally distributed between groups. Programmed primary coronary angioplasty (85% with stent placement) was performed after blood sampling in 9 patients with SA (75%), 12 patients with UA (92%), and 14 patients with MI (100%).
Controls (9 men and 3 women, mean age 58±4 years) were patients with angiographic documentation of absence of CAD (4 patients with chest pain, 4 patients with valvular disease, and 4 patients with dilated cardiomyopathy).
To characterize the cell origins of the microparticles, we included 16 additional consecutive patients with angina and angiographic documentation of CAD (5 with SA, mean age 63±5 years, and 11 with ACS, mean age 62±4 years) and 6 noncoronary patients (5 with valvular disease and 1 with dilated cardiomyopathy, mean age 65±8 years).
Isolation of the Circulating Microparticles and Determination of
Their Procoagulant Potential
Blood samples were collected before any mechanical intervention
at admission (day 0), except in 6 patients with MI, in whom blood
sampling was performed at day 8 after the coronary event.
Microparticles were captured by immobilized annexin V, and
the anionic phospholipid content was determined by a prothrombinase
assay as previously described in detail.9 10 We verified
that the method used for the capture of microparticles did not allow
the capture of PS-containing lipoproteins.
Determination of the Cell Origins of Circulating
Microparticles
Microparticles were captured by specific antibodies (anti-CD3,
anti-CD11a, anti-CD31, anti-CD146, and anti-GP Ib).9 10
The morphology of circulating microparticles was recently
published.11 12
Statistical Analysis
Results are expressed as mean±SEM. Comparisons between groups
were made by a 1-way ANOVA. Simple regression analysis was
performed to analyze the relation between values of
microparticles captured with anti-CD31, anti-CD146, or anti-GP Ib
antibodies. A value of P<0.05 was considered statistically
significant.
| Results |
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Two patients experienced recurrent ischemic syndromes during hospitalization: 1 patient in the UA group developed recurrent documented myocardial ischemia, and 1 in the MI group had reocclusion of his stented culprit coronary artery. Interestingly, these 2 patients had very high baseline levels of circulating procoagulant microparticles (35.6 and 62.3 nmol/L PS, respectively).
Circulating microparticles captured with anti-CD146 or anti-CD31
antibody were elevated in patients with ACS (compared with both stable
coronary and noncoronary patients), whereas those
captured with anti-GP Ib, anti-CD3, or anti-CD11a were not
(Table
). The values obtained with
anti-CD31 antibody were not correlated with those obtained with anti-GP
Ib antibody but were highly correlated with those obtained with
anti-CD146 antibody (P<0.001). Given that there were almost
no CD3-bearing microparticles, these findings indicate that
microparticles captured with anti-CD31 or anti-CD146 were most likely
of endothelial origin.
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| Discussion |
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Soejima et al14 recently reported that plasma TF antigen levels are significantly elevated in patients with UA compared with those measured in patients with SA and are associated with a poor prognosis. However, these authors did not measure TF activity, nor did they determine the origin of the TF antigen. Our findings extend those of Soejima et al and suggest that the procoagulant potential of the circulating blood is, at least in part, related to the presence of elevated levels of circulating procoagulant microparticles. Moreover, the prevalence of microparticles bearing CD146 and CD31 suggests a potentially important role for acute endothelial injury in this process. This may reflect the endothelial erosion at the site of plaque disruption, the endothelial injury on exposure of plaque microvessels to inflammatory cells, and/or the endothelial injury associated with myocardial ischemia. It should be noted that our data do not exclude the possibility that a certain amount of circulating microparticles may also originate from other cell types, including smooth muscle cells and cardiomyocytes.
The detection of elevated levels of circulating procoagulant microparticles 8 days after the acute ischemic syndrome is in line with the observation of persistent intracoronary thrombi 24 hours to 30 days after the ischemic episode.15 In the present study, the 2 patients who experienced documented recurrent myocardial ischemia or coronary reocclusion with reinfarction had very high basal levels of circulating microparticles. This observation suggests that the level of circulating microparticles could be useful as an indicator of the persistence or recurrence of thrombus and therefore as a prognostic marker of the recurrence of ischemic events. This hypothesis needs to be tested in a large multicenter study.
In addition to their direct effect in promotion and amplification of the coagulation cascade, the circulating microparticles may also act in a variety of inflammatory processes16 17 and may be responsible for dissemination of the procoagulant and proinflammatory potentials to sites remote from the microenvironment of their formation.8
In conclusion, high levels of procoagulant microparticles are present in the circulating blood of patients with ACS and may participate in the generation and perpetuation of intracoronary thrombi. The high levels of circulating microparticles of endothelial origin suggest an important role for endothelial injury in thrombus formation.
| Acknowledgments |
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Received October 29, 1999; revision received December 31, 1999; accepted January 10, 2000.
| References |
|---|
|
|
|---|
2.
Lee RT, Libby P. The unstable atheroma.
Arterioscler Thromb Vasc Biol. 1997;17:18591867.
3.
Toschi V, Gallo G, Lettino M, Fallon JT, Gertz SD,
Fernandez-Ortiz A, Chesebro JH, Badimon L, Nemerson Y, Fuster V,
Badimon JJ. Tissue factor modulates thrombogenicity of human
atherosclerotic plaques. Circulation. 1997;95:594599.
4. Ardissino D, Merlini PA, Ariens R, Coppola R, Bramucci E, Mannucci PM. Tissue-factor antigen and activity in human coronary atherosclerotic plaques. Lancet. 1997;349:769771.[Medline] [Order article via Infotrieve]
5.
Pei G, Powers DD, Lentz BR. Specific contribution of
different phospholipid surfaces to the activation of prothrombin by the
fully assembled prothrombinase. J Biol Chem. 1993;268:32263233.
6.
Martin SJ, Reutelingsperger CPM, McGahon AJ, Rader JA,
van Schie RCA, LaFace DM, Green DR. Early redistribution of plasma
membrane phosphatidylserine is a general feature of
apoptosis regardless of the initiating stimulus: inhibition by
overexpression of Bcl-2 and Abl. J Exp Med. 1995;182:15451556.
7.
Casciola-Rosen LA, Rosen A, Petri M, Schlissel M.
Surface blebs on apoptotic cells are sites of enhanced
procoagulant activity: implications for coagulation events and
antigenic spread in systemic lupus
erythematosus. Proc Natl Acad Sci
U S A. 1996;93:16241629.
8. Satta N, Toti F, Feugeas O, Bohbot A, Dachary-Prigent J, Eschwège V, Hedman H, Freyssinet JM. Monocyte vesiculation is a possible mechanism for dissemination of membrane-associated procoagulant activities and adhesion molecules after stimulation by lipopolysaccharide. J Immunol. 1994;153:32453255.[Abstract]
9.
Mallat Z, Hugel B, Ohan J, Lesèche G, Freyssinet
JM, Tedgui A. Shed membrane microparticles with procoagulant potential
in human atherosclerotic plaques: a role for apoptosis in
plaque thrombogenicity. Circulation. 1999;99:348353.
10. Aupeix K, Hugel B, Martin T, Bischoff P, Lill H, Pasquali JL, Freyssinet JM. The significance of shed membrane particles during programmed cell death in vitro, and in vivo, in HIV-1 infection. J Clin Invest. 1997;99:15461554.[Medline] [Order article via Infotrieve]
11. Freyssinet J-M, Toti F, Hugel B, Gidon-Jeangirard C, Kunzelmann C, Martìnez MC, Meyer D. Apoptosis in vascular disease. Thromb Haemost. 1999;82:127135.
12. Combes V, Simon AC, Grau GE, Arnoux D, Camoin L, Sabatier F, Mutin M, Sanmarco M, Sampol J, Dignat-George F. In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant. J Clin Invest. 1999;104:93102.[Medline] [Order article via Infotrieve]
13. Keularts IM, Beguin S, de Zwaan C, Hemker HC. Treatment with a GPIIb/IIIa antagonist inhibits thrombin generation in platelet rich plasma from patients. Thromb Haemost. 1998;80:370371.[Medline] [Order article via Infotrieve]
14.
Soejima H, Ogawa H, Yasue H, Kaikita K, Nishiyama K,
Misumi K, Takazoe K, Miyao Y, Yoshimura M, Kugiyama K, Nakamura S,
Tsuji I, Kumeda K. Heightened tissue factor associated with tissue
factor pathway inhibitor and prognosis in patients with
unstable angina. Circulation. 1999;99:29082913.
15.
Van Belle E, Lablanche JM, Bauters C, Renaud N,
McFadden EP, Bertrand ME. Coronary angioscopic findings in the
infarct-related vessel within 1 month of acute myocardial infarction:
natural history and the effect of thrombolysis.
Circulation. 1998;97:2633.
16. Barry OP, Praticò D, Lawson JA, FitzGerald GA. Transcellular activation of platelets and endothelial cells by bioactive lipids in platelet microparticles. J Clin Invest. 1997;99:21182127.[Medline] [Order article via Infotrieve]
17. Fourcade O, Simon MF, Viode C, Rugani FL, Ragab A, Fournie B, Sarda L, Chap H. Secretory phospholipase A2 generates the novel lipid mediator lysophosphatidic acid in membrane microvesicles shed from activated cells. Cell. 1995;80:919927.[Medline] [Order article via Infotrieve]
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||||
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||||
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I. Ott Soluble Tissue Factor Emerges From Inflammation Circ. Res., June 24, 2005; 96(12): 1217 - 1218. [Full Text] [PDF] |
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H. Koga, S. Sugiyama, K. Kugiyama, K. Watanabe, H. Fukushima, T. Tanaka, T. Sakamoto, M. Yoshimura, H. Jinnouchi, and H. Ogawa Elevated Levels of VE-Cadherin-Positive Endothelial Microparticles in Patients With Type 2 Diabetes Mellitus and Coronary Artery Disease J. Am. Coll. Cardiol., May 17, 2005; 45(10): 1622 - 1630. [Abstract] [Full Text] [PDF] |
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V. Llorente-Cortes and L. Badimon LDL Receptor-Related Protein and the Vascular Wall: Implications for Atherothrombosis Arterioscler Thromb Vasc Biol, March 1, 2005; 25(3): 497 - 504. [Abstract] [Full Text] [PDF] |
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M. C. Martinez, A. Tesse, F. Zobairi, and R. Andriantsitohaina Shed membrane microparticles from circulating and vascular cells in regulating vascular function Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1004 - H1009. [Abstract] [Full Text] [PDF] |
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J. L. Yu, L. May, V. Lhotak, S. Shahrzad, S. Shirasawa, J. I. Weitz, B. L. Coomber, N. Mackman, and J. W. Rak Oncogenic events regulate tissue factor expression in colorectal cancer cells: implications for tumor progression and angiogenesis Blood, February 15, 2005; 105(4): 1734 - 1741. [Abstract] [Full Text] [PDF] |
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G. Busch, I. Seitz, B. Steppich, S. Hess, R. Eckl, A. Schomig, and I. Ott Coagulation Factor Xa Stimulates Interleukin-8 Release in Endothelial Cells and Mononuclear Leukocytes: Implications in Acute Myocardial Infarction Arterioscler Thromb Vasc Biol, February 1, 2005; 25(2): 461 - 466. [Abstract] [Full Text] [PDF] |
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S. M. Day, J. L. Reeve, B. Pedersen, D. M Farris, D. D. Myers, M. Im, T. W. Wakefield, N. Mackman, and W. P. Fay Macrovascular thrombosis is driven by tissue factor derived primarily from the blood vessel wall Blood, January 1, 2005; 105(1): 192 - 198. [Abstract] [Full Text] [PDF] |
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P. R. Moreno and V. Fuster New aspects in the pathogenesis of diabetic atherothrombosis J. Am. Coll. Cardiol., December 21, 2004; 44(12): 2293 - 2300. [Abstract] [Full Text] [PDF] |
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A. C. Ferreira, A. A. Peter, A. J. Mendez, J. J. Jimenez, L. M. Mauro, J. A. Chirinos, R. Ghany, S. Virani, S. Garcia, L. L. Horstman, et al. Postprandial Hypertriglyceridemia Increases Circulating Levels of Endothelial Cell Microparticles Circulation, December 7, 2004; 110(23): 3599 - 3603. [Abstract] [Full Text] [PDF] |
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M. Hristov, W. Erl, S. Linder, and P. C. Weber Apoptotic bodies from endothelial cells enhance the number and initiate the differentiation of human endothelial progenitor cells in vitro Blood, November 1, 2004; 104(9): 2761 - 2766. [Abstract] [Full Text] [PDF] |
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K. G. Birukov, V. N. Bochkov, A. A. Birukova, K. Kawkitinarong, A. Rios, A. Leitner, A. D. Verin, G. M. Bokoch, N. Leitinger, and Joe. G.N. Garcia Epoxycyclopentenone-Containing Oxidized Phospholipids Restore Endothelial Barrier Function via Cdc42 and Rac Circ. Res., October 29, 2004; 95(9): 892 - 901. [Abstract] [Full Text] [PDF] |
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O. Gasser and J. A. Schifferli Activated polymorphonuclear neutrophils disseminate anti-inflammatory microparticles by ectocytosis Blood, October 15, 2004; 104(8): 2543 - 2548. [Abstract] [Full Text] [PDF] |
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V. Llorente-Cortes, M. Otero-Vinas, S. Camino-Lopez, O. Llampayas, and L. Badimon Aggregated Low-Density Lipoprotein Uptake Induces Membrane Tissue Factor Procoagulant Activity and Microparticle Release in Human Vascular Smooth Muscle Cells Circulation, July 27, 2004; 110(4): 452 - 459. [Abstract] [Full Text] [PDF] |
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O. Aras, A. Shet, R. R. Bach, J. L. Hysjulien, A. Slungaard, R. P. Hebbel, G. Escolar, B. Jilma, and N. S. Key Induction of microparticle- and cell-associated intravascular tissue factor in human endotoxemia Blood, June 15, 2004; 103(12): 4545 - 4553. [Abstract] [Full Text] [PDF] |
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T. Suhara, K. Fukuo, O. Yasuda, M. Tsubakimoto, Y. Takemura, H. Kawamoto, T. Yokoi, M. Mogi, T. Kaimoto, and T. Ogihara Homocysteine Enhances Endothelial Apoptosis via Upregulation of Fas-Mediated Pathways Hypertension, June 1, 2004; 43(6): 1208 - 1213. [Abstract] [Full Text] [PDF] |
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E. Durand, A. Scoazec, A. Lafont, J. Boddaert, A. Al Hajzen, F. Addad, M. Mirshahi, M. Desnos, A. Tedgui, and Z. Mallat In Vivo Induction of Endothelial Apoptosis Leads to Vessel Thrombosis and Endothelial Denudation: A Clue to the Understanding of the Mechanisms of Thrombotic Plaque Erosion Circulation, June 1, 2004; 109(21): 2503 - 2506. [Abstract] [Full Text] [PDF] |
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S. Rajagopalan, E. C. Somers, R. D. Brook, C. Kehrer, D. Pfenninger, E. Lewis, A. Chakrabarti, B. C. Richardson, E. Shelden, W. J. McCune, et al. Endothelial cell apoptosis in systemic lupus erythematosus: a common pathway for abnormal vascular function and thrombosis propensity Blood, May 15, 2004; 103(10): 3677 - 3683. [Abstract] [Full Text] [PDF] |
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T. Aprahamian, I. Rifkin, R. Bonegio, B. Hugel, J.-M. Freyssinet, K. Sato, J. J. Castellot Jr., and K. Walsh Impaired Clearance of Apoptotic Cells Promotes Synergy between Atherogenesis and Autoimmune Disease J. Exp. Med., April 19, 2004; 199(8): 1121 - 1131. [Abstract] [Full Text] [PDF] |
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S. Martin, A. Tesse, B. Hugel, M. C. Martinez, O. Morel, J.-M. Freyssinet, and R. Andriantsitohaina Shed Membrane Particles From T Lymphocytes Impair Endothelial Function and Regulate Endothelial Protein Expression Circulation, April 6, 2004; 109(13): 1653 - 1659. [Abstract] [Full Text] [PDF] |
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L. M. L. Bezerra and S. G. Filler Interactions of Aspergillus fumigatus with endothelial cells: internalization, injury, and stimulation of tissue factor activity Blood, March 15, 2004; 103(6): 2143 - 2149. [Abstract] [Full Text] [PDF] |
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H J Ankersmit, T Weber, J Auer, G Roth, M Brunner, E Kvas, B Moser, S Spreitzer, E Lassnig, E Maurer, et al. Increased serum concentrations of soluble CD95/Fas and caspase 1/ICE in patients with acute angina Heart, February 1, 2004; 90(2): 151 - 154. [Abstract] [Full Text] [PDF] |
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H. Carp, R. Dardik, A. Lubetsky, O. Salomon, R. Eskaraev, E. Rosenthal, and A. Inbal Prevalence of circulating procoagulant microparticles in women with recurrent miscarriage: a case-controlled study Hum. Reprod., January 1, 2004; 19(1): 191 - 195. [Abstract] [Full Text] [PDF] |
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F. D. Kolodgie, A. Petrov, R. Virmani, N. Narula, J. W. Verjans, D. K. Weber, D. Hartung, N. Steinmetz, J. L. Vanderheyden, M. A. Vannan, et al. Targeting of Apoptotic Macrophages and Experimental Atheroma With Radiolabeled Annexin V: A Technique With Potential for Noninvasive Imaging of Vulnerable Plaque Circulation, December 23, 2003; 108(25): 3134 - 3139. [Abstract] [Full Text] [PDF] |
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A. Minagar and J S. Alexander Blood-brain barrier disruption in multiple sclerosis Multiple Sclerosis, December 1, 2003; 9(6): 540 - 549. [Abstract] [PDF] |
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T. A. Sanders, S. E. Berry, and G. J Miller Influence of triacylglycerol structure on the postprandial response of factor VII to stearic acid-rich fats Am. J. Clinical Nutrition, April 1, 2003; 77(4): 777 - 782. [Abstract] [Full Text] [PDF] |
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A. Sambola, J. Osende, J. Hathcock, M. Degen, Y. Nemerson, V. Fuster, J. Crandall, and J. J. Badimon Role of Risk Factors in the Modulation of Tissue Factor Activity and Blood Thrombogenicity Circulation, February 25, 2003; 107(7): 973 - 977. [Abstract] [Full Text] [PDF] |
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P. K. Shah Mechanisms of plaque vulnerability and rupture J. Am. Coll. Cardiol., February 19, 2003; 41(4_Suppl_S): 15S - 22S. [Abstract] [Full Text] [PDF] |
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R. A. Preston, W. Jy, J. J. Jimenez, L. M. Mauro, L. L. Horstman, M. Valle, G. Aime, and Y. S. Ahn Effects of Severe Hypertension on Endothelial and Platelet Microparticles Hypertension, February 1, 2003; 41(2): 211 - 217. [Abstract] [Full Text] [PDF] |
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D. Proudfoot, J.D. Davies, J.N. Skepper, P.L. Weissberg, and C.M. Shanahan Acetylated Low-Density Lipoprotein Stimulates Human Vascular Smooth Muscle Cell Calcification by Promoting Osteoblastic Differentiation and Inhibiting Phagocytosis Circulation, December 10, 2002; 106(24): 3044 - 3050. [Abstract] [Full Text] [PDF] |
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V. Schachinger and A. M. Zeiher Atherogenesis--recent insights into basic mechanisms and their clinical impact Nephrol. Dial. Transplant., December 1, 2002; 17(12): 2055 - 2064. [Full Text] [PDF] |
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M. Diamant, R. Nieuwland, R. F. Pablo, A. Sturk, J. W.A. Smit, and J. K. Radder Elevated Numbers of Tissue-Factor Exposing Microparticles Correlate With Components of the Metabolic Syndrome in Uncomplicated Type 2 Diabetes Mellitus Circulation, November 5, 2002; 106(19): 2442 - 2447. [Abstract] [Full Text] [PDF] |
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C. Kluft, R. Kleemann, and M.P.M. de Maat How best to counteract the enemies? By controlling inflammation in the coronary circulation Eur. Heart J. Suppl., November 1, 2002; 4(suppl_G): G53 - G65. [Abstract] [PDF] |
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V. Balasubramanian, E. Grabowski, A. Bini, and Y. Nemerson Platelets, circulating tissue factor, and fibrin colocalize in ex vivo thrombi: real-time fluorescence images of thrombus formation and propagation under defined flow conditions Blood, September 26, 2002; 100(8): 2787 - 2792. [Abstract] [Full Text] [PDF] |
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F. Sabatier, P. Darmon, B. Hugel, V. Combes, M. Sanmarco, J.-G. Velut, D. Arnoux, P. Charpiot, J.-M. Freyssinet, C. Oliver, et al. Type 1 And Type 2 Diabetic Patients Display Different Patterns of Cellular Microparticles Diabetes, September 1, 2002; 51(9): 2840 - 2845. [Abstract] [Full Text] [PDF] |
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R. Gonzalez-Conejero, J. Corral, V. Roldan, C. Martinez, F. Marin, J. Rivera, J. A. Iniesta, M. L. Lozano, P. Marco, and V. Vicente A common polymorphism in the annexin V Kozak sequence (-1C>T) increases translation efficiency and plasma levels of annexin V, and decreases the risk of myocardial infarction in young patients Blood, August 28, 2002; 100(6): 2081 - 2086. [Abstract] [Full Text] [PDF] |
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E. I. Lev, J. D. Marmur, M. Zdravkovic, J. I. Osende, J. Robbins, J. A. Delfin, M. Richard, E. Erhardtsen, M. S. Thomsen, A. M. Lincoff, et al. Antithrombotic Effect of Tissue Factor Inhibition by Inactivated Factor VIIa: An Ex Vivo Human Study Arterioscler Thromb Vasc Biol, June 1, 2002; 22(6): 1036 - 1041. [Abstract] [Full Text] [PDF] |
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D. Bonderman, A. Teml, J. Jakowitsch, C. Adlbrecht, M. Gyongyosi, W. Sperker, H. Lass, W. Mosgoeller, D. H. Glogar, P. Probst, et al. Coronary no-reflow is caused by shedding of active tissue factor from dissected atherosclerotic plaque Blood, April 15, 2002; 99(8): 2794 - 2800. [Abstract] [Full Text] [PDF] |
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J. Huber, A. Vales, G. Mitulovic, M. Blumer, R. Schmid, J. L. Witztum, B. R. Binder, and N. Leitinger Oxidized Membrane Vesicles and Blebs From Apoptotic Cells Contain Biologically Active Oxidized Phospholipids That Induce Monocyte-Endothelial Interactions Arterioscler Thromb Vasc Biol, January 1, 2002; 22(1): 101 - 107. [Abstract] [Full Text] [PDF] |
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C. M. Boulanger, A. Scoazec, T. Ebrahimian, P. Henry, E. Mathieu, A. Tedgui, and Z. Mallat Circulating Microparticles From Patients With Myocardial Infarction Cause Endothelial Dysfunction Circulation, November 27, 2001; 104(22): 2649 - 2652. [Abstract] [Full Text] [PDF] |
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Z. Mallat and A. Tedgui Current Perspective on the Role of Apoptosis in Atherothrombotic Disease Circ. Res., May 25, 2001; 88(10): 998 - 1003. [Abstract] [Full Text] [PDF] |
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A. Minagar, W. Jy, J. J. Jimenez, W. A. Sheremata, L. M. Mauro, W. W. Mao, L. L. Horstman, and Y. S. Ahn Elevated plasma endothelial microparticles in multiple sclerosis Neurology, May 22, 2001; 56(10): 1319 - 1324. [Abstract] [Full Text] [PDF] |
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A. Tedgui and Z. Mallat Smooth Muscle Cells : Another Source of Tissue Factor-Containing Microparticles in Atherothrombosis? Circ. Res., July 21, 2000; 87(2): 81 - 82. [Full Text] [PDF] |
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J.-R. Nofer, B. Levkau, I. Wolinska, R. Junker, M. Fobker, A. von Eckardstein, U. Seedorf, and G. Assmann Suppression of Endothelial Cell Apoptosis by High Density Lipoproteins (HDL) and HDL-associated Lysosphingolipids J. Biol. Chem., September 7, 2001; 276(37): 34480 - 34485. [Abstract] [Full Text] [PDF] |
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P. Andre, D. Hartwell, I. Hrachovinova, S. Saffaripour, and D. D. Wagner Pro-coagulant state resulting from high levels of soluble P-selectin in blood PNAS, December 5, 2000; 97(25): 13835 - 13840. [Abstract] [Full Text] [PDF] |
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