(Circulation. 2002;105:1890.)
© 2002 American Heart Association, Inc.
Clinical Investigation and Reports |
From the Division of Cardiac Surgery, Toronto General Hospital Research Institute, University of Toronto, Canada.
Correspondence to Dr Donald A.G. Mickle, Toronto Gerneral Hospital, 200 Elizabeth St, Toronto, Canada M5G 2C4, and reprint requests to Dr Subodh Verma (e-mail subodh.verma{at}sympatico.ca).
| Abstract |
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Methods and Results Saphenous vein endothelial cells (HSVECs) were incubated with human recombinant CRP (25 µg/mL, 24 hours) and the expression of vascular cell adhesion molecule (VCAM-1), intracellular adhesion molecule (ICAM-1), and monocyte chemoattractant chemokine-1 was determined. The effects of CRP on LDL uptake were assessed in macrophages using immunofluorescent labeling of CD32 and CD14. In each study, the effect of endothelin antagonism (bosentan) and IL-6 inhibition (monoclonal anti-IL-6 antibodies) was examined. The effects of CRP on the secretion of ET-1 and IL-6 from HSVECs were also evaluated. Incubation of HSVECs with recombinant human CRP resulted in a marked increase in ICAM-1 and VCAM-1 expression (P<0.001). Likewise, CRP caused a significant increase in monocyte chemoattractant chemokine-1 production, a key mediator of leukocyte transmigration (P<0.001). CRP caused a marked and sustained increase in native LDL uptake by macrophages (P<0.05). These proatherosclerotic effects of CRP were mediated, in part, via increased secretion of ET-1 and IL-6 (P<0.01) and were attenuated by both bosentan and IL-6 antagonism (P<0.01).
Conclusions CRP actively promotes a proatherosclerotic and proinflammatory phenotype. These effects are mediated, in part, via the production of ET-1 and IL-6 and are attenuated by mixed ETA/B receptor antagonism and IL-6 inhibition. Bosentan may be useful in decreasing CRP-mediated vascular disease.
Key Words: protein, C-reactive cell adhesion molecules endothelium atherosclerosis endothelin
| Introduction |
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In the present study, we hypothesized that the proatherosclerotic and inflammatory effects of CRP may be mediated in part via increased production or action of the potent endothelium-derived vasoactive factor endothelin-1 (ET-1) and the inflammatory cytokine interleukin-6 (IL-6).
| Methods |
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Monocyte Chemoattractant Protein-1, ET-1, and IL-6 Secretion
HSVECs were cultured as described above. HSVECs at passages 2 through 5 (n=7 per group) were incubated with 25 µg/mL human recombinant CRP (Calbiochem) for 24 hours in the presence and absence of bosentan and anti-human IL-6 antibody (RandD Systems) for 2 hours before being incubated with CRP, as described above. Culture supernatants were collected, and the secretion of monocyte chemoattractant protein-1 (MCP-1) and IL-6 was assessed by sandwich ELISA (RandD Systems). ET-1 secretion into the culture supernatant assessed with a commercial enzyme immunoassay kit was used to assess ET-1 production (American Research Products, Inc). All determinations were performed in triplicate.
Monocyte Isolation and Macrophage LDL Uptake Assay
Human monocytes were isolated from the heparinized blood of healthy volunteers, as previously described.17 Cells were adjusted with RPMI-10% FBS (Gibco) to a density of 5.0x107 cells/mL. Monocyte-derived macrophages were prepared by in vitro incubation of purified monocytes on 12-well tissue culture plates for 7 days at 37°C, 5% CO2 in RPMI-10% FBS.
A protocol adapted from Zwaka et al16 was used to assess LDL uptake in macrophages. Briefly, 900 mg/L human recombinant CRP (Calbiochem) was coincubated with 1000 mg/dL native LDL (Sigma) in PBS containing CaCl2 (0.132 g/L) and MgCl2 (0.1 g/L) at room temperature for 15 minutes. The supernatant was diluted in RPMI-10% to a final concentration of 240 mg/L CRP and 250 mg/dL LDL. The CRP/LDL coincubate was centrifuged at 15 000 rpm for 30 minutes to remove high molecular aggregates and was cooled to 4°C. Substitution with PBS instead of CRP served as a control. Macrophages were incubated for 12 hours in RPMI-2% FBS and were then serum-starved for 3 hours. Cells were washed with PBS (4°C) and incubated with CRP/LDL coincubates at 4°C for 1 hour. In experiments assessing the effects of ET antagonism and IL-6 inhibition, macrophages were pretreated with 10 µmol/L bosentan (Actelion Ltd) or 5 µg/mL anti-human IL-6 antibody (RandD Systems) for 3 hours before being incubated with CRP/LDL coincubates, as described above. Macrophages were stained for CD32 and CD14 (macrophage marker) using monoclonal FITC-conjugated anti-CD32 and monoclonal PE-conjugated anti-CD14 antibodies (Pharmingen), both at a 1:5 dilution. Cells were analyzed using a Beckman Coulter EPICS XL flow cytometer with EXPO32 ADC software. Forward and side scatter characteristics were used to gate cell population and exclude extraneous debris. A total of 10 000 positively staining cells were analyzed.
Statistical Analysis
All data are presented as mean±SD of separate experiments. Differences between group means were determined by a one-way ANOVA followed by a Newman Keuls test for post hoc comparisons. Values of P<0.05 were considered significant.
| Results |
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Cell Adhesion Molecule Expression
Figures 3 and 4 depict the effects of human recombinant CRP (25 µg/mL) on HSVEC ICAM-1 and VCAM-1 expression after a 24-hour incubation period in the presence of 10% FBS. Incubation with CRP resulted in a marked increase in ICAM-1 and VCAM-1 expression (P<0.001). In cells preincubated with bosentan or IL-6 antibody, ICAM-1 and VCAM-1 expression was significantly attenuated (P<0.01). The effects of coincubation with bosentan and IL-6 antibody were additive (P<0.05). Expression is related to mean florescence intensity from forward scatter and side scatter. Expression of PECAM-1 (CD31) was used as a marker of endothelial cell viability, and the mean fluorescence intensity was corrected for PECAM-1.
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MCP-1 Secretion in HSVECs
Figure 5 depicts the effects of human recombinant CRP (25 µg/mL) on HSVEC secretion of MCP-1 in the presence and absence of 2 hours of pretreatment with bosentan (10 µmol/L) or an anti-human IL-6neutralizing antibody (5 µg/mL) after a 24-hour incubation in the presence of 10% FBS. Incubation with CRP in the absence of any intervention resulted in a marked increase in the secretion of MCP-1 (from 1140±90 to 2600±210 pg/mL, P<0.01). When CRP was added to cells preincubated with bosentan or antiIL-6 antibody, the increased secretion of MCP-1 was attenuated (P<0.05). Attenuation of CRP-induced MCP-1 production was greater during coincubation with bosentan and antiIL-6 antibody (P<0.01, Figure 5), additionally supporting the theory that CRP may stimulate ET-1 and IL-6 concurrently.
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Macrophage LDL Uptake
To investigate the role of ET-1 and IL-6 as mediators of CRP-induced LDL uptake, we conducted a flow-cytometric 2-color analysis of anti-CD32 and anti-CD14 that was previously used to demonstrate internalization and colocalization of macrophage CD32 with CRP/LDL complexes.16 Figure 6 depicts the effect of human recombinant CRP (25 µg/mL) on human macrophage LDL uptake in the presence and absence of 3-hour pretreatment with bosentan (10 µmol/L) or an anti-human IL-6neutralizing antibody (5 µg/mL). Analysis of flow cytometric data of macrophages incubated with LDL alone revealed an 89.5% positive stain for CD32 and CD14, whereas after incubation with LDL and CRP, a 61.3% positive stain for CD32 and CD14 was observed; this represents a significant uptake of LDL (P<0.05). When CRP/LDL was added to cells preincubated with bosentan, CRP-mediated LDL uptake observed previously was significantly attenuated (P<0.05). Similarly, when CRP/LDL was added to cells preincubated with anti-human IL-6 antibody, CRP-mediated LDL uptake was also significantly attenuated (P<0.05), suggesting that the effects of CRP on LDL uptake were mediated, in part, by the action of IL-6 and ET-1.
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| Discussion |
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Inflammation Plays a Central Role in the Development of Atherosclerosis
The formation of an atherosclerotic lesion begins as a fatty streak underlying the endothelium of arteries.18 Recruitment of monocyte-derived macrophages and their subsequent uptake of oxidized LDL cholesterol are the major cellular events contributing to fatty-streak formation. The recruitment of monocytes to lesion-prone sites of arteries is tightly regulated by cell adhesion molecules that are expressed on the surface of endothelial cells in response to inflammatory stimuli. The selectin family of glycoproteins, P-selectin and E-selectin, are important in regulating the first step of neutrophilendothelial cell interaction, termed leukocyte rolling. Once the leukocytes make contact with the dysfunctional endothelium, they are activated and proceed to the second step of firm adherence to the endothelium. This process is regulated by a pair of receptors, one on the surface of the neutrophil, and adhesion molecules on the endothelium (ICAM-1 and VCAM-1).1820 The final step, ie, neutrophil migration into the subendothelial space, is likely to be stimulated in part by oxidized LDL, which can directly attract monocytes and also induce the expression of chemokines such as MCP-1 by endothelial cells. This chemoattractant chemokine is highly expressed in human atherosclerotic lesions and is believed to be the principal stimulus for monocyte recruitment into the arterial wall. Studies have demonstrated that mice lacking the leukocyte receptor for MCP-1 (CCR2) exhibit marked reductions in atherosclerotic lesion formation, providing compelling evidence that MCP-1 and its receptor play a critical role in the initiation of atherosclerosis.18 The development of macrophage foam cells that contain massive amounts of cholesterol esters is a hallmark of both early and late atherosclerotic lesions. Accumulation of cholesterol in these cells is mediated primarily via uptake of LDL cholesterol via a complex series of well-regulated events. The transition from the relatively simple fatty streak to the more complex atherosclerotic lesion is characterized by the immigration of smooth muscle cells from the medial layer and into the intimal or subendothelial space. Intimal smooth muscle cells can synthesize extracellular matrix proteins that lead to the development of the fibrous cap. This phase of lesion development is influenced by interactions between monocyte/macrophages and T cells that result in a broad range of cellular and humoral responses similar to those observed in states of chronic inflammation.1821
It is now well accepted that inflammation plays a central role in the development of atherosclerosis and its complications.13,18,19 Chronic inflammation results in endothelial dysfunction and facilitates the interactions between modified lipoproteins, monocyte-derived macrophages, T cells, and the normal cellular elements of the arterial wall. Indeed, inflammatory processes have been implicated in each facet of atherogenesis described above. Inflammatory markers such as CRP and IL-6 not only participate in lesion formation but also alter plaque architecture in favor of rupture.13
CRP: Marker and Mediator of Inflammation and Atherosclerosis
Several large-scale epidemiological studies have shown that plasma levels of high-sensitivity CRP are a strong independent predictor of endothelial dysfunction, future myocardial dysfunction, stroke, peripheral artery disease, and vascular death among individuals without known cardiovascular disease.113 In addition, among patients with known coronary artery disease, levels of CRP have been linked with increased vascular event rates. The latter observations are important, because recent evidence suggests that inflammation is a critical determinant of plaque stability and rupture. A direct comparison of the magnitude of relative risk (compared with traditional risk factors) revealed that CRP is the single strongest predictor of risk, with a relative risk of 4.4 for the highest versus lowest quartile.13 Furthermore, in a multivariate analysis, only CRP and the ratio of total to HDL cholesterol proved to have independent predictive value once age, smoking status, obesity, hypertension, obesity, diabetes, and family history were accounted for.2 More recently, elevated CRP levels have been demonstrated to predict the risk of death and myocardial infarction in patients undergoing percutaneous coronary intervention (PCI) after adjustment of baseline values known to influence early events after PCI.22 These observations have set the stage for routine CRP assessments to enter conventional atherosclerosis prediction algorithms.
Until recently, CRP was regarded as a nonspecific marker of inflammation versus an active partaker in the process of atherogenesis. Several recent studies have now clearly demonstrated that human CRP, at concentrations known to predict increased vascular event rates, directly induces a proinflammatory and proatherosclerotic phenotype. In human umbilical vein and coronary endothelial cells, CRP has been demonstrated to increase the expression of ICAM-1, VCAM-1, and MCP-1 in a concentration-dependent fashion.14,15 Likewise, CRP has been demonstrated to facilitate native LDL uptake into macrophages, an important step in foam-cell formation.16 CRP may also directly promote monocyte activation by stimulating the release of cytokines such as IL-1b, IL-6, and TNF-
23 and increasing the release of soluble IL-6 receptor.24 Recent evidence shows that C-reactive protein (CRP) is deposited in the arterial intima at sites of atherogenesis.25 Importantly, CRP deposition precedes the appearance of monocytes in early atherosclerotic lesions.25 In addition to the aforementioned proatherogenic actions, CRP is a well-recognized stimulator of the complement system. Complement activation plays an important role in atherogenesis, and CRP has been demonstrated to colocalize with terminal complement complexes in established coronary plaques.26 Lastly, data demonstrating that CRP may exaggerate lipopolysaccharide-mediated activation of endothelial cells and monocytes27,28 additionally strengthen the evidence of a direct effect of CRP on vascular inflammation.
ET-1 and IL-6 May Mediate the Proinflammatory Actions of CRP
The present study adds to the growing body of literature that supports a direct proinflammatory and proatherosclerotic effect of CRP. We demonstrate that CRP may evoke the production of the potent endothelium-derived vasoactive factor ET-1 and the inflammatory cytokine IL-6. Additional evidence to support a role of ET-1 and IL-6 as mediators of the actions of CRP is provided by experiments examining the effects of IL-6 inhibition and ET receptor antagonism. Both bosentan and antiIL-6 antibodies attenuated CRP-mediated expression of adhesion molecules, MCP-1 secretion, and macrophage LDL uptake.
Although ET-1 is known to be one of the upstream activators of IL-6,29 we suggest that this may not be the case in HSVECs stimulated with CRP. This conclusion is based on our observation that IL-6 levels are not attenuated during concurrent ET receptor blockade. Hence, it is possible that CRP stimulates the production of IL-6 and ET-1 concurrently in a parallel fashion. Alternatively, it is possible that CRP quenches an unidentified inhibitory factor, such as nitric oxide, known to decrease IL-6 and ET-1 secretion. It is also plausible that CRP serves to activate nuclear factor-
B (NF-
B), with the resultant increase in expression of IL-6, ET-1, ICAM-1, VCAM-1, and other NF-
Bregulated systems. This however, remains to be determined.
ET-1 is one of the most potent endogenous vasoconstrictors and mediates a host of responses, including endothelial dysfunction, vasomotor contraction, leukocyte and platelet activation, and cellular proliferation.30 Additionally, it augments the vascular actions of other vasoactive substances, such as A-II, norepinephrine, and serotonin. In the endothelial cell, ET-1 is produced by preproET under the influence of the endothelin-converting enzyme. ET-1 exerts its biological effects via interacting with endothelin receptors (ETA and ETB), with the former mediating the bulk of the vascular actions.
IL-6 is an important inflammatory cytokine that has been implicated in the pathogenesis and clinical course of atherosclerotic vascular disease.13,31 IL-6 is known to be secreted from several cell types, including endothelial cells, macrophages, lymphocytes, and adipocytes, and exerts its biological actions through a complex yet well-defined fashion. The IL-6 receptor complex consists of two membrane-bound glycoproteins, an 80-kD ligand-binding component (termed IL-6R), and a 130-kD signal-transducing component (termed gp130).31 Although IL-6 may bind the IL-6R and elicit a biological response, it also activates a soluble IL-6R (sIL-6R). The activated IL-6/sIL-6R complex serves as a potent agonist that binds the signal-transducing component of the membrane-bound receptor gp130 with high affinity. Through this mechanism, IL-6 is believed to potentiate its own biological activity and exert effects in cells that lack the IL-6R per se. Indeed the IL-6/sIL-6R complex has been demonstrated to stimulate leukocyte recruitment and promote endothelial cell inflammatory responses.32,33
The aforementioned discussion assumes importance, because recent studies have demonstrated that CRP is a physiological regulator of sIL-6R shedding in human neutrophils and markedly increases the formation of the sIL-6R/IL-6 complex.24 Data from the present study suggest that CRP may also function to increase IL-6 secretion from endothelial cells. By stimulating endothelial production of IL-6 and neutrophil sIL-6R shedding, CRP may serve to markedly exaggerate the actions of IL-6 at the level of the endothelium. Because IL-6 is a potent hepatic stimulus for CRP, increased vascular production may represent a positive feedback mechanism for the continued production of CRP from the liver.
Limitations
Results from the present study demonstrate that the proinflammatory actions of CRP can be attenuated during mixed ETA/B receptor blockade; however, they do not allow us to comment on the relative importance of ETA versus ETB antagonism in this effect. Second, the experiments were performed in venous endothelial cells and hence cannot be unequivocally extrapolated to other vascular endothelial cells.
Conclusions
Results from the present study support a growing body of evidence suggesting that CRP exerts direct proinflammatory and proatherosclerotic effects (Figure 7). CRP directly facilitates endothelial cell adhesion molecule expression, MCP-1 production, and macrophage LDL uptake. Importantly, these effects seem to occur, in part, through an ET-1dependent and IL-6dependent fashion and are attenuated during pharmacological antagonism with bosentan and antiIL-6 antibodies. This is also the first study to examine the role of CRP in saphenous vein endothelial cells and hence may have implications for saphenous vein graft atherosclerosis in patients undergoing coronary artery bypass graft surgery. Clearly, understanding the mechanisms and mediators of the proinflammatory effects of CRP may yield new therapeutic targets, such as bosentan, to predict, prevent, and treat cardiovascular disease.
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| Acknowledgments |
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| Footnotes |
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Received January 23, 2002; revision received February 25, 2002; accepted February 26, 2002.
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D. Ramzy, V. Rao, L. C. Tumiati, N. Xu, R. Sheshgiri, J. Jackman, D. H. Delgado, and H. J. Ross Endothelin-1 accentuates the proatherosclerotic effects associated with C-reactive protein J. Thorac. Cardiovasc. Surg., May 1, 2007; 133(5): 1137 - 1146. [Abstract] [Full Text] [PDF] |
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R. J. Bisoendial, J. J. P. Kastelein, S. L. M. Peters, J. H. M. Levels, R. Birjmohun, J. I. Rotmans, D. Hartman, J. C. M. Meijers, M. Levi, and E. S. G. Stroes Effects of CRP infusion on endothelial function and coagulation in normocholesterolemic and hypercholesterolemic subjects J. Lipid Res., April 1, 2007; 48(4): 952 - 960. [Abstract] [Full Text] [PDF] |
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O. Schlager, M. Exner, W. Mlekusch, S. Sabeti, J. Amighi, P. Dick, O. Wagner, R. Koppensteiner, E. Minar, and M. Schillinger C-Reactive Protein Predicts Future Cardiovascular Events in Patients With Carotid Stenosis Stroke, April 1, 2007; 38(4): 1263 - 1268. [Abstract] [Full Text] [PDF] |
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N. R. Madamanchi and M. S. Runge Mitochondrial Dysfunction in Atherosclerosis Circ. Res., March 2, 2007; 100(4): 460 - 473. [Abstract] [Full Text] [PDF] |
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C. Rocker, D. E. Manolov, E. V. Kuzmenkina, K. Tron, H. Slatosch, J. Torzewski, and G. U. Nienhaus Affinity of C-Reactive Protein toward Fc{gamma}RI Is Strongly Enhanced by the {gamma}-Chain Am. J. Pathol., February 1, 2007; 170(2): 755 - 763. [Abstract] [Full Text] [PDF] |
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H. D. Sesso, L. Wang, J. E. Buring, P. M Ridker, and J. M. Gaziano Comparison of Interleukin-6 and C-Reactive Protein for the Risk of Developing Hypertension in Women Hypertension, February 1, 2007; 49(2): 304 - 310. [Abstract] [Full Text] [PDF] |
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M. Rodriguez-Yanez, M. Castellanos, M. Blanco, M. M. Garcia, F. Nombela, J. Serena, R. Leira, I. Lizasoain, A. Davalos, and J. Castillo New-onset hypertension and inflammatory response/poor outcome in acute ischemic stroke Neurology, December 12, 2006; 67(11): 1973 - 1978. [Abstract] [Full Text] [PDF] |
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H. Fujii, S.-H. Li, P. E. Szmitko, P. W.M. Fedak, and S. Verma C-Reactive Protein Alters Antioxidant Defenses and Promotes Apoptosis in Endothelial Progenitor Cells Arterioscler Thromb Vasc Biol, November 1, 2006; 26(11): 2476 - 2482. [Abstract] [Full Text] [PDF] |
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S. Kapiotis, G. Holzer, G. Schaller, M. Haumer, H. Widhalm, D. Weghuber, B. Jilma, G. Roggla, M. Wolzt, K. Widhalm, et al. A Proinflammatory State Is Detectable in Obese Children and Is Accompanied by Functional and Morphological Vascular Changes Arterioscler Thromb Vasc Biol, November 1, 2006; 26(11): 2541 - 2546. [Abstract] [Full Text] [PDF] |
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S. Devaraj, B. Davis, S. I. Simon, and I. Jialal CRP promotes monocyte-endothelial cell adhesion via Fc{gamma} receptors in human aortic endothelial cells under static and shear flow conditions Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1170 - H1176. [Abstract] [Full Text] [PDF] |
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Y. Zhong, S.-H. Li, S.-M. Liu, P. E. Szmitko, X.-Q. He, P. W.M. Fedak, and S. Verma C-Reactive Protein Upregulates Receptor for Advanced Glycation End Products Expression in Human Endothelial Cells Hypertension, September 1, 2006; 48(3): 504 - 511. [Abstract] [Full Text] [PDF] |
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M. Suzuki, M. Saito, T. Nagai, H. Saeki, and Y. Kazatani Systemic Versus Coronary Levels of Inflammation in Acute Coronary Syndromes Angiology, August 1, 2006; 57(4): 459 - 463. [Abstract] [PDF] |
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P. Joppa, D. Petrasova, B. Stancak, and R. Tkacova Systemic Inflammation in Patients With COPD and Pulmonary Hypertension. Chest, August 1, 2006; 130(2): 326 - 333. [Abstract] [Full Text] [PDF] |
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D. Ramzy, V. Rao, L. C. Tumiati, N. Xu, R. Sheshgiri, S. Miriuka, D. H. Delgado, and H. J. Ross Elevated Endothelin-1 Levels Impair Nitric Oxide Homeostasis Through a PKC-Dependent Pathway Circulation, July 4, 2006; 114(1_suppl): I-319 - I-326. [Abstract] [Full Text] [PDF] |
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B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, I. Jialal, B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, and I. Jialal The Verdict Is Still Out Circulation, May 2, 2006; 113(17): 2128 - 2151. [Full Text] [PDF] |
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I. Montero, J. Orbe, N. Varo, O. Beloqui, J. I. Monreal, J. A. Rodriguez, J. Diez, P. Libby, and J. A. Paramo C-Reactive Protein Induces Matrix Metalloproteinase-1 and -10 in Human Endothelial Cells: Implications for Clinical and Subclinical Atherosclerosis J. Am. Coll. Cardiol., April 4, 2006; 47(7): 1369 - 1378. [Abstract] [Full Text] [PDF] |
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R. L. Benza, B. K. Rayburn, J. A. Tallaj, C. S. Coffey, L. J. Pinderski, S. V. Pamoukian, and R. C. Bourge Efficacy of bosentan in a small cohort of adult patients with pulmonary arterial hypertension related to congenital heart disease. Chest, April 1, 2006; 129(4): 1009 - 1015. [Abstract] [Full Text] [PDF] |
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N. Dhaun, J. Goddard, and DavidJ. Webb The Endothelin System and Its Antagonism in Chronic Kidney Disease J. Am. Soc. Nephrol., April 1, 2006; 17(4): 943 - 955. [Abstract] [Full Text] [PDF] |
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D. M. Mannino, G. Watt, D. Hole, C. Gillis, C. Hart, A. McConnachie, G. Davey Smith, M. Upton, V. Hawthorne, D. D. Sin, et al. The natural history of chronic obstructive pulmonary disease. Eur. Respir. J., March 1, 2006; 27(3): 627 - 643. [Full Text] [PDF] |
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P. Davis, G. Valacchi, E. Pagnin, Q. Shao, H. B. Gross, L. Calo, and W. Yokoyama Walnuts Reduce Aortic ET-1 mRNA Levels in Hamsters Fed a High-Fat, Atherogenic Diet J. Nutr., February 1, 2006; 136(2): 428 - 432. [Abstract] [Full Text] [PDF] |
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A. M Carter Inflammation, thrombosis and acute coronary syndromes Diabetes and Vascular Disease Research, October 1, 2005; 2(3): 113 - 121. [Abstract] [PDF] |
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R. F. Bonvini, T. Hendiri, and E. Camenzind Inflammatory response post-myocardial infarction and reperfusion: a new therapeutic target? Eur. Heart J. Suppl., October 1, 2005; 7(suppl_I): I27 - I36. [Abstract] [Full Text] [PDF] |
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T. Nakakuki, M. Ito, H. Iwasaki, Y. Kureishi, R. Okamoto, N. Moriki, M. Kongo, S. Kato, N. Yamada, N. Isaka, et al. Rho/Rho-Kinase Pathway Contributes to C-Reactive Protein-Induced Plasminogen Activator Inhibitor-1 Expression in Endothelial Cells Arterioscler Thromb Vasc Biol, October 1, 2005; 25(10): 2088 - 2093. [Abstract] [Full Text] [PDF] |
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T. Khreiss, L. Jozsef, L. A. Potempa, and J. G. Filep Loss of Pentameric Symmetry in C-Reactive Protein Induces Interleukin-8 Secretion Through Peroxynitrite Signaling in Human Neutrophils Circ. Res., September 30, 2005; 97(7): 690 - 697. [Abstract] [Full Text] [PDF] |
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I. S. Anand, R. Latini, V. G. Florea, M. A. Kuskowski, T. Rector, S. Masson, S. Signorini, P. Mocarelli, A. Hester, R. Glazer, et al. C-Reactive Protein in Heart Failure: Prognostic Value and the Effect of Valsartan Circulation, September 6, 2005; 112(10): 1428 - 1434. [Abstract] [Full Text] [PDF] |
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S. S. Nerurkar, P. J. McDevitt, G. F. Scott, K. O. Johanson, R. N. Willette, and T.-L. Yue Lipopolysaccharide (LPS) Contamination Plays the Real Role in C-Reactive Protein-Induced IL-6 Secretion From Human Endothelial Cells In Vitro Arterioscler Thromb Vasc Biol, September 1, 2005; 25(9): e136 - e136. [Full Text] [PDF] |
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C. Cipolletta, K. E. Ryan, E. V. Hanna, and E. R. Trimble Activation of Peripheral Blood CD14+ Monocytes Occurs in Diabetes Diabetes, September 1, 2005; 54(9): 2779 - 2786. [Abstract] [Full Text] [PDF] |
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M. Satoh, M. Nakamura, T. Akatsu, Y. Shimoda, I. Segawa, and K. Hiramori C-reactive protein co-expresses with tumor necrosis factor-{alpha} in the myocardium in human dilated cardiomyopathy Eur J Heart Fail, August 1, 2005; 7(5): 748 - 754. [Abstract] [Full Text] [PDF] |
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K. Reifenberg, H.-A. Lehr, D. Baskal, E. Wiese, S. C. Schaefer, S. Black, D. Samols, M. Torzewski, K. J. Lackner, M. Husmann, et al. Role of C-Reactive Protein in Atherogenesis: Can the Apolipoprotein E Knockout Mouse Provide the Answer? Arterioscler Thromb Vasc Biol, August 1, 2005; 25(8): 1641 - 1646. [Abstract] [Full Text] [PDF] |
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A. Trion, M.P.M. de Maat, J.W. Jukema, A. van der Laarse, M.C. Maas, E.H. Offerman, L.M. Havekes, A.J. Szalai, H.M.G. Princen, and J.J. Emeis No Effect of C-Reactive Protein on Early Atherosclerosis Development in Apolipoprotein E*3-Leiden/Human C-Reactive Protein Transgenic Mice Arterioscler Thromb Vasc Biol, August 1, 2005; 25(8): 1635 - 1640. [Abstract] [Full Text] [PDF] |
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J. Amar, J.-B. Ruidavets, J.-C. Peyrieux, J.-M. Mallion, J. Ferrieres, M. E. Safar, and B. Chamontin C-Reactive Protein Elevation Predicts Pulse Pressure Reduction in Hypertensive Subjects Hypertension, July 1, 2005; 46(1): 151 - 155. [Abstract] [Full Text] [PDF] |
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C. Arnaud, F. Burger, S. Steffens, N. R. Veillard, T. H. Nguyen, D. Trono, and F. Mach Statins Reduce Interleukin-6-Induced C-Reactive Protein in Human Hepatocytes: New Evidence for Direct Antiinflammatory Effects of Statins Arterioscler Thromb Vasc Biol, June 1, 2005; 25(6): 1231 - 1236. [Abstract] [Full Text] [PDF] |
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K. E. Taylor, J. C. Giddings, and C. W. van den Berg C-Reactive Protein-Induced In Vitro Endothelial Cell Activation Is an Artefact Caused by Azide and Lipopolysaccharide Arterioscler Thromb Vasc Biol, June 1, 2005; 25(6): 1225 - 1230. [Abstract] [Full Text] [PDF] |
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M. Schillinger, M. Exner, W. Mlekusch, S. Sabeti, J. Amighi, R. Nikowitsch, E. Timmel, B. Kickinger, C. Minar, M. Pones, et al. Inflammation and Carotid Artery--Risk for Atherosclerosis Study (ICARAS) Circulation, May 3, 2005; 111(17): 2203 - 2209. [Abstract] [Full Text] [PDF] |
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E. Qamirani, Y. Ren, L. Kuo, and T. W. Hein C-Reactive Protein Inhibits Endothelium-Dependent NO-Mediated Dilation in Coronary Arterioles by Activating p38 Kinase and NAD(P)H Oxidase Arterioscler Thromb Vasc Biol, May 1, 2005; 25(5): 995 - 1001. [Abstract] [Full Text] [PDF] |
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P. E. Szmitko and S. Verma Antiatherogenic potential of red wine: clinician update Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2023 - H2030. [Abstract] [Full Text] [PDF] |
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D. C. W. Lau, B. Dhillon, H. Yan, P. E. Szmitko, and S. Verma Adipokines: molecular links between obesity and atheroslcerosis Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2031 - H2041. [Abstract] [Full Text] [PDF] |
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B. R. Clapp, G. M. Hirschfield, C. Storry, J. R. Gallimore, R. P. Stidwill, M. Singer, J. E. Deanfield, R. J. MacAllister, M. B. Pepys, P. Vallance, et al. Inflammation and Endothelial Function: Direct Vascular Effects of Human C-Reactive Protein on Nitric Oxide Bioavailability Circulation, March 29, 2005; 111(12): 1530 - 1536. [Abstract] [Full Text] [PDF] |
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H.-K. Yip, C.-L. Hang, C.-Y. Fang, Y.-K. Hsieh, C.-H. Yang, W.-C. Hung, and C.-J. Wu Level of High-Sensitivity C-Reactive Protein Is Predictive of 30-Day Outcomes in Patients With Acute Myocardial Infarction Undergoing Primary Coronary Intervention Chest, March 1, 2005; 127(3): 803 - 808. [Abstract] [Full Text] [PDF] |
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A. Chait, C. Y. Han, J. F. Oram, and J. W. Heinecke Thematic review series: The Immune System and Atherogenesis. Lipoprotein-associated inflammatory proteins: markers or mediators of cardiovascular disease? J. Lipid Res., March 1, 2005; 46(3): 389 - 403. [Abstract] [Full Text] [PDF] |
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K. J.E. Sattler, J. E. Woodrum, O. Galili, M. Olson, S. Samee, F. B. Meyer, X.-Y. Zhu, L. O. Lerman, and A. Lerman Concurrent Treatment With Renin-Angiotensin System Blockers and Acetylsalicylic Acid Reduces Nuclear Factor {kappa}B Activation and C-Reactive Protein Expression in Human Carotid Artery Plaques Stroke, January 1, 2005; 36(1): 14 - 20. [Abstract] [Full Text] [PDF] |
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L. Zhang, K. Peppel, L. Brian, L. Chien, and N. J. Freedman Vein Graft Neointimal Hyperplasia Is Exacerbated by Tumor Necrosis Factor Receptor-1 Signaling in Graft-Intrinsic Cells Arterioscler Thromb Vasc Biol, December 1, 2004; 24(12): 2277 - 2283. [Abstract] [Full Text] [PDF] |
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D. E. Manolov, C. Rocker, V. Hombach, G. U. Nienhaus, and J. Torzewski Ultrasensitive Confocal Fluorescence Microscopy of C-Reactive Protein Interacting With Fc{gamma}RIIa Arterioscler Thromb Vasc Biol, December 1, 2004; 24(12): 2372 - 2377. [Abstract] [Full Text] [PDF] |
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H.-K. Yip, C.-J. Wu, H.-W. Chang, C.-H. Yang, K.-H. Yeh, S. Chua, and M. Fu Levels and Values of Serum High-Sensitivity C-Reactive Protein Within 6 Hours After the Onset of Acute Myocardial Infarction Chest, November 1, 2004; 126(5): 1417 - 1422. [Abstract] [Full Text] [PDF] |
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L. Li, N. Roumeliotis, T. Sawamura, and G. Renier C-Reactive Protein Enhances LOX-1 Expression in Human Aortic Endothelial Cells: Relevance of LOX-1 to C-Reactive Protein-Induced Endothelial Dysfunction Circ. Res., October 29, 2004; 95(9): 877 - 883. [Abstract] [Full Text] [PDF] |
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S. Fazel, R. D. Weisel, and S. Verma A novel technique to assess flow-mediated vasodilation J. Am. Coll. Cardiol., October 6, 2004; 44(7): 1478 - 1480. [Full Text] [PDF] |
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D. D. Sin, P. Lacy, E. York, and S. F. P. Man Effects of Fluticasone on Systemic Markers of Inflammation in Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., October 1, 2004; 170(7): 760 - 765. [Abstract] [Full Text] [PDF] |
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M. Lambert, E. E. Delvin, G. Paradis, J. O'Loughlin, J. A. Hanley, and E. Levy C-Reactive Protein and Features of the Metabolic Syndrome in a Population-Based Sample of Children and Adolescents Clin. Chem., October 1, 2004; 50(10): 1762 - 1768. [Abstract] [Full Text] [PDF] |
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D. Fliser, K. Buchholz, H. Haller, and for the EUropean Trial on Olmesartan and Pravastat Antiinflammatory Effects of Angiotensin II Subtype 1 Receptor Blockade in Hypertensive Patients With Microinflammation Circulation, August 31, 2004; 110(9): 1103 - 1107. [Abstract] [Full Text] [PDF] |
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S. P. Zhao, L. Liu, Y. C. Cheng, M. H. Shishehbor, M. H. Liu, D. Q. Peng, and Y. L. Li Xuezhikang, an Extract of Cholestin, Protects Endothelial Function Through Antiinflammatory and Lipid-Lowering Mechanisms in Patients With Coronary Heart Disease Circulation, August 24, 2004; 110(8): 915 - 920. [Abstract] [Full Text] [PDF] |
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M. Tobu, O. Iqbal, D. Fareed, M. Chatha, D. Hoppensteadt, V. Bansal, and J. Fareed Erythropoietin-Induced Thrombosis as a Result of Increased Inflammation and Thrombin Activatable Fibrinolytic Inhibitor Clinical and Applied Thrombosis/Hemostasis, July 1, 2004; 10(3): 225 - 232. [Abstract] [PDF] |
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I. Jialal, S. Devaraj, and S. K. Venugopal C-Reactive Protein: Risk Marker or Mediator in Atherothrombosis? Hypertension, July 1, 2004; 44(1): 6 - 11. [Abstract] [Full Text] [PDF] |
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K. Amann, M.-L. Gross, and E. Ritz Pathophysiology Underlying Accelerated Atherogenesis in Renal Disease: Closing in on the Target J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1664 - 1666. [Full Text] [PDF] |
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R. Tauman, A. Ivanenko, L. M. O'Brien, and D. Gozal Plasma C-Reactive Protein Levels Among Children With Sleep-Disordered Breathing Pediatrics, June 1, 2004; 113(6): e564 - e569. [Abstract] [Full Text] |
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S. Verma, M. A. Kuliszewski, S.-H. Li, P. E. Szmitko, L. Zucco, C.-H. Wang, M. V. Badiwala, D. A.G. Mickle, R. D. Weisel, P. W.M. Fedak, et al. C-Reactive Protein Attenuates Endothelial Progenitor Cell Survival, Differentiation, and Function: Further Evidence of a Mechanistic Link Between C-Reactive Protein and Cardiovascular Disease Circulation, May 4, 2004; 109(17): 2058 - 2067. [Abstract] [Full Text] [PDF] |
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S. Wan, A. P. C. Yim, J. L. Johnson, N. Shukla, G. D. Angelini, F. C. T. Smith, M. R. Dashwood, and J. Y. Jeremy The endothelin 1A receptor antagonist BSF 302146 is a potent inhibitor of neointimal and medial thickening in porcine saphenous vein-carotid artery interposition grafts J. Thorac. Cardiovasc. Surg., May 1, 2004; 127(5): 1317 - 1322. [Abstract] [Full Text] [PDF] |
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S. Verma, P. E. Szmitko, and E. T.H. Yeh C-Reactive Protein: Structure Affects Function Circulation, April 27, 2004; 109(16): 1914 - 1917. [Full Text] [PDF] |
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T. Khreiss, L. Jozsef, L. A. Potempa, and J. G. Filep Conformational Rearrangement in C-Reactive Protein Is Required for Proinflammatory Actions on Human Endothelial Cells Circulation, April 27, 2004; 109(16): 2016 - 2022. [Abstract] [Full Text] [PDF] |
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R. Arroyo-Espliguero, P. Avanzas, J. Cosin-Sales, G. Aldama, C. Pizzi, and J. C. Kaski C-reactive protein elevation and disease activity in patients with coronary artery disease Eur. Heart J., March 1, 2004; 25(5): 401 - 408. [Abstract] [Full Text] [PDF] |
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S.-H. Li, P. E. Szmitko, R. D. Weisel, C.-H. Wang, P. W.M. Fedak, R.-K. Li, D. A.G. Mickle, and S. Verma C-Reactive Protein Upregulates Complement-Inhibitory Factors in Endothelial Cells Circulation, February 24, 2004; 109(7): 833 - 836. [Abstract] [Full Text] [PDF] |
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A. Paul, K. W.S. Ko, L. Li, V. Yechoor, M. A. McCrory, A. J. Szalai, and L. Chan C-Reactive Protein Accelerates the Progression of Atherosclerosis in Apolipoprotein E-Deficient Mice Circulation, February 10, 2004; 109(5): 647 - 655. [Abstract] [Full Text] [PDF] |
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G. J. Blake, N. Rifai, J. E. Buring, and P. M Ridker Blood Pressure, C-Reactive Protein, and Risk of Future Cardiovascular Events Circulation, December 16, 2003; 108(24): 2993 - 2999. [Abstract] [Full Text] [PDF] |
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H. D. Sesso, J. E. Buring, N. Rifai, G. J. Blake, J. M. Gaziano, and P. M. Ridker C-Reactive Protein and the Risk of Developing Hypertension JAMA, December 10, 2003; 290(22): 2945 - 2951. [Abstract] [Full Text] [PDF] |
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S. Verma, M. V. Badiwala, R. D. Weisel, S.-H. Li, C.-H. Wang, P. W. M. Fedak, R.-K. Li, and D. A. G. Mickle C-reactive protein activates the nuclear factor-{kappa}B signal transduction pathway in saphenous vein endothelial cells: implications for atherosclerosis and restenosis J. Thorac. Cardiovasc. Surg., December 1, 2003; 126(6): 1886 - 1891. [Abstract] [Full Text] [PDF] |
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S. Verma and P. E. Szmitko Coxibs and the endothelium J. Am. Coll. Cardiol., November 19, 2003; 42(10): 1754 - 1756. [Full Text] [PDF] |
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M. Schillinger, M. Exner, J. Amighi, W. Mlekusch, S. Sabeti, H. Rumpold, O. Wagner, and E. Minar Joint Effects of C-Reactive Protein and Glycated Hemoglobin in Predicting Future Cardiovascular Events of Patients With Advanced Atherosclerosis Circulation, November 11, 2003; 108(19): 2323 - 2328. [Abstract] [Full Text] [PDF] |
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R. Arroyo-Espliguero, N. Mollichelli, P. Avanzas, E. Zouridakis, V. R Newey, D. K Nassiri, and J. C. Kaski Chronic inflammation and increased arterial stiffness in patients with cardiac syndrome X Eur. Heart J., November 2, 2003; 24(22): 2006 - 2011. [Abstract] [Full Text] [PDF] |
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G.M. Hirschfield and M.B. Pepys C-reactive protein and cardiovascular disease: new insights from an old molecule QJM, November 1, 2003; 96(11): 793 - 807. [Abstract] [Full Text] [PDF] |
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G. J. Blake and P. M. Ridker C-reactive protein: a surrogate risk marker or mediator of atherothrombosis? Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2003; 285(5): R1250 - R1252. [Full Text] [PDF] |
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S. Verma and E. T. H. Yeh C-reactive protein and atherothrombosis--Beyond a biomarker: an actual partaker of lesion formation Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2003; 285(5): R1253 - R1256. [Full Text] [PDF] |
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S. Verma, M. R. Buchanan, and T. J. Anderson Endothelial Function Testing as a Biomarker of Vascular Disease Circulation, October 28, 2003; 108(17): 2054 - 2059. [Full Text] [PDF] |
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