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(Circulation. 2002;105:2332.)
© 2002 American Heart Association, Inc.

Clinician Update

Fundamentals of Reperfusion Injury for the Clinical Cardiologist

Subodh Verma, MD, PhD; Paul W.M. Fedak, MD; Richard D. Weisel, MD; Jagdish Butany, MD; Vivek Rao, MD, PhD; Andrew Maitland, MD; Ren-Ke Li, MD, PhD; Bikramjit Dhillon, BSc; Terrence M. Yau, MD

From the Division of Cardiac Surgery, University of Toronto, Toronto, Canada.

Correspondence to Richard D. Weisel, MD, FRCSC, Professor of Surgery, Chair, Division of Cardiac Surgery, EN 14-215, 200 Elizabeth St, Toronto General Hospital. Toronto, Ontario, Canada, M5G 2C4. E-mail richard.weisel{at}uhn.on.ca

	Introduction

Top Introduction References

 
Case presentation: S.B. is a 48-year-old man who suffered an acute anterior myocardial infarction and received fibrinolytic therapy. The patient died 12 hours after reperfusion. K.R. is a 68-year-old diabetic woman who underwent conventional coronary artery bypass graft surgery and developed low output syndrome after reperfusion postoperatively. V.A. is a 55-year-old man who developed a stunned myocardium after percutaneous coronary reperfusion. What is reperfusion injury, and why is it important?

Reperfusion of coronary flow is necessary to resuscitate the ischemic or hypoxic myocardium. Timely reperfusion facilitates cardiomyocyte salvage and decreases cardiac morbidity and mortality. Reperfusion of an ischemic area may result, however, in paradoxical cardiomyocyte dysfunction, a phenomenon termed "reperfusion injury." Modalities for reperfusion include not only thrombolysis, but also percutaneous coronary intervention (PCI), coronary artery bypass grafting (CABG), and cardiac transplantation. Reperfusion injury has been observed in each of these situations. We discuss here the fundamental principles of reperfusion injury from a mechanistic and pharmacological standpoint.

What is reperfusion injury, and why is it important?
The myocardium can tolerate brief periods (up to 15 minutes) of severe and even total myocardial ischemia without resultant cardiomyocyte death. Although the cardiomyocytes suffer ischemic injury, the damage is reversible with prompt arterial reperfusion. Indeed, such transient periods of ischemia are encountered in the clinical situations of angina, coronary vasospasm, and balloon angioplasty, and are not associated with concomitant myocyte cell death.^1,2 With increasing duration and severity of ischemia, however, greater cardiomyocyte damage can develop, with a predisposition to a spectrum of reperfusion-associated pathologies, collectively called reperfusion injury.³ Reperfusion injury results in myocyte damage through myocardial stunning, microvascular and endothelial injury, and irreversible cell damage or necrosis (termed lethal reperfusion injury; Figure 1).^3,4

View larger version (122K): [in this window] [in a new window]   Figure 1. Mechanisms and mediators of reperfusion injury. Reperfusion strategies are required to resuscitate the ischemic myocardium. In the clinical realm, these include reperfusion for acute myocardial infarction (AMI) and angioplasty and after CABG surgery and exercise- or stress-induced ischemia. Reperfusion injury results from several complex and interdependent mechanisms that involve the production of reactive oxygen species, alterations in intracellular calcium handling, microvascular and endothelial cell dysfunction, altered myocardial metabolism, and activation of neutrophils, platelets and complement. Reperfusion injury is manifested as stunned myocardium, reversible microvascular injury, and, rarely, lethal myocyte necrosis.

Myocardial stunning is the best-established manifestation of reperfusion injury.^5,6 It is defined as "prolonged postischemic dysfunction of viable tissue salvaged by reperfusion,"^1,2,7 and was initially described by Heyndrickx et al in 1975.⁸ In this scenario, reperfusion of either a globally or regionally ischemic myocardial tissue results in a period of prolonged, yet reversible, contractile dysfunction. The myocardium is essentially "stunned" and requires a prolonged period of time before complete functional recovery. The clinical correlate of a stunned myocardium can be found after reperfusion of a globally ischemic myocardium (cardiac arrest during cardiac surgery), or in the setting of regional ischemia and reperfusion (PCI, thrombolysis, unstable angina, and stress- or exercise-induced angina).^1,2,5

Microvascular dysfunction is another manifestation of reperfusion injury.^9–11 Reperfusion causes marked endothelial cell dysfunction, which results in vasoconstriction, platelet and leukocyte activation, increased oxidant production, and increased fluid and protein extravasation (discussed below). Although rare, severe microvascular dysfunction may limit adequate perfusion after reperfusion, a phenomenon termed "no-reflow".

Reperfusion of a severely ischemic myocardium may also result in myocyte death and necrosis (lethal reperfusion injury). This usually occurs in cardiomyocytes that have been severely injured by ischemia but also may develop in reversibly injured myocytes. A disruptive type of necrosis, termed contraction band necrosis (Figure 2), has been documented and is ascribed to massive myofibril contraction after reperfusion-induced calcium reentry (Figure 2). This form of reperfusion injury is the most severe and is clearly irreversible.

View larger version (117K): [in this window] [in a new window]   Figure 2. A, Left ventricular free wall from a 48-year-old male who received fibrinolysis for acute anterior myocardial infarction and died 12 hours after reperfusion. The left ventricular myocardium showed dark brown discoloration in the papillary muscle and free wall consistent with reperfusion-related hemorrhage (RI). B, Histological section of myocardium from the same patient showing acute ischemic changes with margination of polymorphonuclear leukocytes (PMN) and interstitial edema (white arrow). In addition, myofibrils show thinning and wavy pattern consistent with reperfusion injury (black arrow). C, High-power representation of the box in B showing neutrophil "tethering" and transmigration (arrowhead). Neutrophil activation plays a prominent role in reperfusion injury. D, Histological section from left ventricular myocardium demonstrating extensive contraction band change (black arrows) and extensive extravasation of red blood cells (arrowhead). Figure courtesy of Dr F. Schoen, Brigham and Women’s Hospital, Boston, Mass. E, Histological section of left ventricular myocardium after acute myocardial infarction without reperfusion. Extensive PMN infiltrate consistent with an 24- to 48-hour-old infarct.

Evidence of stunned myocardium has been extensively documented after reperfusion of an acute myocardial infarction, the deflation of angioplasty balloon, cessation of exercise in patients with coronary artery disease, reperfusion after cardiopulmonary bypass, and reperfusion after ischemic stress induced by dobutamine or dipyridamole.^{1,2,4–6,12–15} Stunning also may be an important causative factor in the development of ischemic cardiomyopathy, wherein repeated episodes of myocardial ischemia and reperfusion may lead to the development of heart failure.⁵

What are the mediators of reperfusion injury?
Several mechanisms and mediators of reperfusion injury have been described. The most frequently cited include oxygen free radicals, intracellular calcium overload, endothelial and microvascular dysfunction, and altered myocardial metabolism.^{9–11,16–18}

Oxygen Free Radicals
The production of excessive quantities of reactive oxygen species is an important mechanism of reperfusion injury. Molecular oxygen, when reintroduced into a previously ischemic myocardium, undergoes a sequential reduction leading to the formation of oxygen free radicals. A landmark study by Bolli and colleagues¹⁹ showed that potent oxidant radicals, such as superoxide anion, hydroxyl radical, and peroxynitrite, are produced within the first few minutes of reflow and play a crucial role in the development of reperfusion injury. Oxygen free radicals also can be generated from sources other than reduction of molecular oxygen. These sources include enzymes, such as xanthine oxidase, cytochrome oxidase, and cyclooxygenase, and the oxidation of catecholamines.

Reperfusion is also a potent stimulus for neutrophil activation and accumulation,¹⁷ which in turn serve as potent stimuli for reactive oxygen species production. Oxygen-derived free radicals produce damage by reacting with polyunsaturated fatty acids, resulting in the formation of lipid peroxides and hydroperoxides that damage the sarcolemma and impair the function of membrane-bound enzyme systems. Free radicals stimulate the endothelial release of platelet activating factor, which attracts more neutrophils and amplifies the production of oxidant radicals and the degree of reperfusion injury. Reactive oxygen species also quench nitric oxide, exaggerating endothelial injury and microvascular dysfunction. In addition to an increased production, there is also a relative deficiency in endogenous oxidant scavenging enzymes, which further exaggerates free radical–mediated cardiac dysfunction.

Endothelial Dysfunction and Microvascular Injury
Reperfusion results in marked endothelial cell dysfunction.^9,18 Endothelium-dependent vasodilatation is impaired, whereas the responses to endothelium-dependent vasoconstrictors are exaggerated. Increased production of potent vasoconstrictors, such as endothelin-1 and oxygen free radicals, increases coronary vasoconstriction and reduces blood flow. Furthermore, endothelial dysfunction facilitates the expression of a prothrombotic phenotype characterized by platelet and neutrophil activation, important mediators of reperfusion injury. Once neutrophils make contact with the dysfunctional endothelium, they are activated, and in a series of well-defined steps (rolling, firm adherence, and transmigration) they migrate into areas of tissue injury through endothelial cell junctions^9,17,18 (Figure 2).

Alterations in Calcium Handling
Changes in intracellular calcium homeostasis play an important role in the development of reperfusion injury.¹⁶ Ischemia and reperfusion are associated with an increase in intracellular calcium; this effect may be related to increased sarcolemmal calcium entry through L-type calcium channels or may be secondary to alterations in sarcoplasmic reticulum calcium cycling. In addition to intracellular calcium overload, alterations in myofilament sensitivity to calcium have been implicated in reperfusion injury. Activation of calcium-dependent proteases (calpain I) with resultant myofibril proteolysis has been suggested to underscore reperfusion injury, as has proteolysis of troponin I.^20,21

Altered Myocardial Metabolism
Reperfusion of an ischemic myocardium results in altered myocardial metabolism, which in turn may contribute to delayed functional recovery. For example, cardioplegic arrest and aortic cross-clamping during cardiac surgery induce anaerobic myocardial metabolism with a net production of lactate.²² Importantly, lactate release persists during reperfusion, suggesting a delayed recovery of normal aerobic metabolism.²² Persistent lactate production after reperfusion predicts postoperative ventricular dysfunction requiring intra-aortic balloon pump support.²² Likewise, the activity of mitochondrial pyruvate dehydrogenase (PDH) is inhibited by 40% after ischemia and remains depressed for up to 30 minutes after reperfusion.^23,24 In addition, the recovery of postischemic myocardial function is dependent on the recovery of PDH activity. These results suggest that persistent anaerobic metabolism may be an important contributor to inadequate postoperative ventricular function; improving the recovery of aerobic myocardial metabolism during reperfusion may serve as an important target for reperfusion injury. Interventions that improve the transition from anaerobic to aerobic myocardial metabolism (insulin, adenosine) facilitate the rapid recovery of aerobic metabolism and left ventricular function after post–cardiac surgery reperfusion.²⁵

Endogenous Protective Mechanisms
The myocardium is the source of endogenous protective mechanisms that are stimulated during reperfusion. These endogenous cardioprotective strategies serve to counter the deleterious mechanisms described above. In many instances, however, they are insufficient to prevent reperfusion injury. The most important endogenous protective mechanisms are adenosine production, opening of ATP-sensitive potassium channels (K_ATP), and release of NO.²⁶ Although the details of endogenous cardioprotection are beyond the scope of this update, it is important to note that these mechanisms have been exploited from pharmacological and therapeutic standpoints (discussed below).

What is the influence of cardiovascular risk factors on reperfusion injury?
Cardiovascular risk factors, including hypercholesterolemia, diabetes, and hypertension, have been reported to increase reperfusion injury. Although the exact mechanisms remain unclear, one recurring theme is that increased oxidative stress and endothelial cell dysfunction may underlie risk factor–mediated exacerbation of reperfusion injury.⁹

Which pharmacological strategies attenuate reperfusion injury?
Over the past 2 decades, 1000 interventions have been studied as potential cardioprotective agents in ischemia and reperfusion injury. We limit our discussion to some of the more contemporary approaches.

Inotropic Stimulation of the Reperfused Stunned Heart
It is important to note that the stunned reperfused myocardium is sensitive to inotropic stimulation.^1,2,26 As discussed above, reperfusion injury results in significant desensitization of the myofibrils to calcium; this phenomenon likely is overcome during inotropic stimulation, augmenting contractility. Although inotropic stimulation is not the ideal strategy to counter reperfusion injury, it is effective and is not associated with a worsening of ultimate functional recovery or tissue necrosis. Indeed, transient inotropic support routinely is used for a stunned reperfused myocardium in a variety of settings.

Antioxidants
The central role of oxygen free radicals in the development of reperfusion injury led to a widespread interest in the use of antioxidant therapy to attenuate reperfusion injury. Antioxidants have been tested in several experimental and clinical models with mixed success.²⁶ Despite positive observations in classic models of experimental ischemia and reperfusion, clinical experience with antioxidants has been disappointing. Indeed, therapy with human recombinant superoxide, designed to attenuate angioplasty-induced reperfusion injury, demonstrated no beneficial effects.²⁷ Although this may be related to cell impermeability, this study cast a shadow on the development of antioxidant strategies for reperfusion injury. It is important to note that the major antioxidant for the cardiomyocyte is glutathione peroxidase, not superoxide dismutase. Vitamin E (alpha tocopherol) is the major lipid-soluble antioxidant and requires prolonged and very high levels of oral treatment to achieve cardiac concentrations that are protective from reperfusion injury.

Sodium-Hydrogen Antiport Inhibition
Inhibition of sodium–hydrogen exchange (Na⁺–H⁺) has received much recent attention as a potential cardioprotectant factor.²⁸ Ischemia and reperfusion result in marked intracellular acidosis; this in turn activates the sarcolemmal Na⁺–H⁺ antiport, which facilitates proton extrusion (in exchange for Na⁺).^3,28 The intracellular hypernatremia that develops results in activation of the sodium–calcium (Na⁺–Ca⁺²) exchanger, with resultant increases in [Ca⁺²] _i. Indeed, inhibitors of Na⁺–H⁺ exchange have been demonstrated to exhibit marked cardioprotection in experimental models of ischemia and reperfusion. More recently, the Na⁺–H⁺ inhibitor cariporide was investigated in a large clinical trial involving 11 500 patients (Guard During Ischemia Against Necrosis [GUARDIAN] trial).²⁹ The trial was designed to investigate the potential cardioprotective effects of cariporide in a diverse group of patients receiving reperfusion treatment (unstable angina, non–ST segment elevation myocardial infarction, high risk-PCI, or surgical revascularization). Although the primary end points of death and myocardial infarction were similar between groups, patients subjected to surgical revascularization exhibited a trend (P=0.06) toward improved left ventricular function in the cariporide group. These data suggest that Na⁺–H⁺ inhibition may be beneficial in attenuating myocardial stunning after CAGB surgery.

Stimulating Endogenous Cardioprotectants
As discussed above, adenosine is an endogenous cardioprotectant released during ischemia that exerts its beneficial effects via opening of mitochondrial K_ATP channels through interaction with the A1 and A3 receptors on cardiomyocytes.³⁰ Despite marked beneficial effects of adenosine therapy in experimental models of ischemia and reperfusion, the clinical experience has been limited. Preliminary results of a phase-II clinical trial suggested that adenosine treatment may reduce the requirement for inotropic and/or mechanical support in patients undergoing cardiac surgery.³¹

Accumulating evidence suggests that endogenous myocardial protection may be mediated via opening of mitochondrial K_ATP channels. Pharmacological agents that open K_ATP channels are being evaluated as potential cardioprotective interventions.³

Decreasing reperfusion injury via modulation of nitric oxide bioavailability is an active area of research.³² Nitric oxide may serve to diminish reperfusion injury through improving endothelial function, decreasing platelet and neutrophil activation, and augmenting coronary flow. NO also may exert direct beneficial effects on cardiomyocyte survival (independent of endothelial cells) and may achieve this through the opening of K_ATP channels.³³ It is important to point out that these cardioprotective effects may depend on the magnitude of NO production; excessive NO production may exert marked deleterious effects on functional recovery. Careful dose-ranging studies will be required before developing NO donors for patients receiving reperfusion treatment.

Metabolic Stimulation With Insulin
In an attempt to improve the transition from anaerobic to aerobic myocardial metabolism, the effects of insulin on ischemia and reperfusion injury have been studied. Insulin caused a marked stimulation of PDH activity and prevented the inhibition of PDH activity after reperfusion.²⁴ Furthermore, insulin treatment reduced extracellular lactate release after reperfusion and increased intracellular high-energy phosphate levels. In a randomized, controlled trial comparing insulin cardioplegia versus placebo, insulin produced a more rapid recovery of aerobic metabolism and left ventricular function after reperfusion (cross-clamp release).²⁵

What does the future hold?
The past 2 decades have witnessed several pharmacological interventions designed to limit reperfusion injury. Unfortunately, the success of some agents has been limited to experimental models of ischemia and reperfusion. The lack of a consistent clinical benefit may be related to a variety of factors, including poor clinical trial design, inadequate pharmacokinetic/pharmacodynamic studies, and the complexity of the human in vivo model (compared with classic experimental models of reperfusion injury). It is important to distinguish therapeutic strategies for ischemia versus reperfusion, and it is possible that a combination of agents is required to elicit maximum clinical benefit. The GUARD during Ischemia Against Necrosis (GUARDIAN) trial with cariporide provides further insight into this concept. Preclinical evaluation of cariporide indicated a consistent benefit when used as a preischemic therapy (versus a reperfusion strategy). Hence, it is not surprising that in the GUARDIAN trial, the only cohort that exhibited benefit was the CABG cohort, in which cariporide was instituted before the onset of ischemia.³

In the future, we will witness the development and testing of additional cardioprotective strategies. Some of the areas of intense investigation include the use of endothelin receptor antagonists, tetrahydrobiopterin, and statins. Clinical trials employing a combination of preischemic and prereperfusion strategies are currently in progress to develop the optimal pharmacological approach to limit reperfusion injury.

	References

Top Introduction References

 

1. Kloner RA, Jennings RB. Consequences of brief ischemia: stunning, preconditioning, and their clinical implications: part I. Circulation. 2001; 104: 2981–2989.[Abstract/Free Full Text]
   
2. Kloner RA, Jennings RB. Consequences of brief ischemia: stunning, preconditioning, and their clinical implications: part II. Circulation. 2001; 104: 3158–3167.[Abstract/Free Full Text]
   
3. Yellon DM, Baxter GF. Protecting the ischaemic and reperfused myocardium in acute myocardial infarction: distant dream or near reality? Heart. 2000; 83: 381–387.[Free Full Text]
   
4. Ambrosio G, Tritto I. Reperfusion injury: experimental evidence and clinical implications. Am Heart J. 1999; 138(2 Pt 2): S69–S75.[CrossRef][Medline] [Order article via Infotrieve]
   
5. Kloner RA, Arimie RB, Kay GL, et al. Evidence for stunned myocardium in humans: a 2001 update. Coron Artery Dis. 2001; 12: 349–356.[CrossRef][Medline] [Order article via Infotrieve]
   
6. Ambrosio G, Tritto I. Clinical manifestations of myocardial stunning. Coron Artery Dis. 2001; 12: 357–361.[CrossRef][Medline] [Order article via Infotrieve]
   
7. Braunwald E, Kloner RA. The stunned myocardium: prolonged, postischemic ventricular dysfunction. Circulation. 1982; 66: 1146–1149.[Abstract/Free Full Text]
   
8. Heyndrickx GR, Millard RW, McRitchie RJ, et al. Regional myocardial functional and electrophysiological alterations after brief coronary artery occlusion in conscious dogs. J Clin Invest. 1975; 56: 978–985.
   
9. Granger DN. Ischemia-reperfusion: mechanisms of microvascular dysfunction and the influence of risk factors for cardiovascular disease. Microcirculation. 1999; 6: 167–178.[CrossRef][Medline] [Order article via Infotrieve]
   
10. Park JL, Lucchesi BR. Mechanisms of myocardial reperfusion injury. Ann Thorac Surg. 1999; 68: 1905–1912.[Abstract/Free Full Text]
    
11. Agati L. Microvascular integrity after reperfusion therapy. Am Heart J. 1999; 138(2 Pt 2): S76–S78.[CrossRef][Medline] [Order article via Infotrieve]
    
12. Gerber BL, Wijns W, Vanoverschelde JL, et al. Myocardial perfusion and oxygen consumption in reperfused noninfarcted dysfunctional myocardium after unstable angina: direct evidence for myocardial stunning in humans. J Am Coll Cardiol. 1999; 34: 1939–1946.[Abstract/Free Full Text]
    
13. Kloner RA, Bolli R, Marban E, et al. Medical and cellular implications of stunning, hibernation, and preconditioning: an NHLBI workshop. Circulation. 1998; 97: 1848–1867.[Free Full Text]
    
14. Ambrosio G, Betocchi S, Pace L, et al. Prolonged impairment of regional contractile function after resolution of exercise-induced angina: evidence of myocardial stunning in patients with coronary artery disease. Circulation. 1996; 94: 2455–2464.[Abstract/Free Full Text]
    
15. Weisel RD. Myocardial stunning after coronary bypass surgery. J Card Surg. 1993; 8 (suppl 2): 242–244.[Medline] [Order article via Infotrieve]
    
16. Gross GJ, Kersten JR, Warltier DC. Mechanisms of postischemic contractile dysfunction. Ann Thorac Surg. 1999; 68: 1898–1904.[Abstract/Free Full Text]
    
17. Jordan JE, Zhao ZQ, Vinten-Johansen J. The role of neutrophils in myocardial ischemia-reperfusion injury. Cardiovasc Res. 1999; 43: 860–878.[Abstract/Free Full Text]
    
18. Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol. 2000; 190: 255–266.[CrossRef][Medline] [Order article via Infotrieve]
    
19. Bolli R, Jeroudi MO, Patel BS, et al. Direct evidence that oxygen-derived free radicals contribute to postischemic myocardial dysfunction in the intact dog. Proc Natl Acad Sci U S A. 1989; 86: 4695–4699.[Abstract/Free Full Text]
    
20. Gao WD, Liu Y, Mellgren R, et al. Intrinsic myofilament alterations underlying the decreased contractility of stunned myocardium: a consequence of Ca²⁺-dependent proteolysis? Circ Res. 1996; 78: 455–465.[Abstract/Free Full Text]
    
21. Gao WD, Atar D, Liu Y, et al. Role of troponin I proteolysis in the pathogenesis of stunned myocardium. Circ Res. 1997; 80: 393–399.
    
22. Rao V, Ivanov J, Weisel RD, et al. Lactate release during reperfusion predicts low cardiac output syndrome after coronary bypass surgery. Ann Thorac Surg. 2001; 71: 1925–1930.[Abstract/Free Full Text]
    
23. Merante F, Mickle DA, Weisel RD, et al. Myocardial aerobic metabolism is impaired in a cell culture model of cyanotic heart disease. Am J Physiol. 1998; 275(5 Pt 2): H1673–H1681.[Abstract/Free Full Text]
    
24. Rao V, Merante F, Weisel RD, et al. Insulin stimulates pyruvate dehydrogenase and protects human ventricular cardiomyocytes from simulated ischemia. J Thorac Cardiovasc Surg. 1998; 116: 485–494.[Abstract/Free Full Text]
    
25. Rao V, Borger MA, Weisel RD, et al. Insulin cardioplegia for elective coronary bypass surgery. J Thorac Cardiovasc Surg. 2000; 119: 1176–1184.[Abstract/Free Full Text]
    
26. Przyklenk K. Pharmacologic treatment of the stunned myocardium: the concepts and the challenges. Coron Artery Dis. 2001; 12: 363–369.[CrossRef][Medline] [Order article via Infotrieve]
    
27. Flaherty JT, Pitt B, Gruber JW, et al. Recombinant human superoxide dismutase (h-SOD) fails to improve recovery of ventricular function in patients undergoing coronary angioplasty for acute myocardial infarction. Circulation. 1994; 89: 1982–1991.[Abstract/Free Full Text]
    
28. Karmazyn M. Mechanisms of protection of the ischemic and reperfused myocardium by sodium-hydrogen exchange inhibition. J Thromb Thrombolysis. 1999; 8: 33–38.[CrossRef][Medline] [Order article via Infotrieve]
    
29. Theroux P, Chaitman BR, Danchin N, et al. Inhibition of the sodium-hydrogen exchanger with cariporide to prevent myocardial infarction in high-risk ischemic situations: main results of the GUARDIAN trial. Guard During Ischemia Against Necrosis (GUARDIAN) Investigators. Circulation. 2000; 102: 3032–3038.[Abstract/Free Full Text]
    
30. Vinten-Johansen J, Thourani VH, Ronson RS, et al. Broad-spectrum cardioprotection with adenosine. Ann Thorac Surg. 1999; 68: 1942–1948.[Abstract/Free Full Text]
    
31. Mentzer RM Jr, Birjiniuk V, Khuri S, et al. Adenosine myocardial protection: preliminary results of a phase II clinical trial. Ann Surg. 1999; 229: 643–649.[CrossRef][Medline] [Order article via Infotrieve]
    
32. Vinten-Johansen J, Zhao ZQ, Nakamura M, et al. Nitric oxide and the vascular endothelium in myocardial ischemia-reperfusion injury. Ann N Y Acad Sci. 1999; 874: 354–370.[Abstract/Free Full Text]
    
33. Shiono N, Rao V, Weisel RD, et al. L-arginine protects human heart cells from low-volume anoxia and reoxygenation. Am J Physiol. 2002; 282: H805–H815.[Abstract/Free Full Text]
    

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				C. Kupatt, C. Dessy, R. Hinkel, P. Raake, G. Daneau, C. Bouzin, P. Boekstegers, and O. Feron Heat Shock Protein 90 Transfection Reduces Ischemia-Reperfusion-Induced Myocardial Dysfunction via Reciprocal Endothelial NO Synthase Serine 1177 Phosphorylation and Threonine 495 Dephosphorylation Arterioscler. Thromb. Vasc. Biol., August 1, 2004; 24(8): 1435 - 1441. [Abstract] [Full Text] [PDF]

				O. Turschner, J. D'hooge, C. Dommke, P. Claus, E. Verbeken, I. De Scheerder, B. Bijnens, and G. R Sutherland The sequential changes in myocardial thickness and thickening which occur during acute transmural infarction, infarct reperfusion and the resultant expression of reperfusion injury Eur. Heart J., May 1, 2004; 25(9): 794 - 803. [Abstract] [Full Text] [PDF]

				B. Zingarelli, P. W. Hake, M. O'Connor, A. Denenberg, H. R. Wong, S. Kong, and B. J. Aronow Differential regulation of activator protein-1 and heat shock factor-1 in myocardial ischemia and reperfusion injury: role of poly(ADP-ribose) polymerase-1 Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1408 - H1415. [Abstract] [Full Text] [PDF]

				W. Bao, E. Hu, L. Tao, R. Boyce, R. Mirabile, D. T Thudium, X.-l. Ma, R. N Willette, and T.-l. Yue Inhibition of Rho-kinase protects the heart against ischemia/reperfusion injury Cardiovasc Res, February 15, 2004; 61(3): 548 - 558. [Abstract] [Full Text] [PDF]

				N. A Weimert, W. F Tanke, and J J. Sims Allopurinol as a Cardioprotectant During Coronary Artery Bypass Graft Surgery Ann. Pharmacother., November 1, 2003; 37(11): 1708 - 1711. [Abstract] [Full Text] [PDF]

				Z.-Q. Zhao, J. S. Corvera, M. E. Halkos, F. Kerendi, N.-P. Wang, R. A. Guyton, and J. Vinten-Johansen Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning Am J Physiol Heart Circ Physiol, August 1, 2003; 285(2): H579 - H588. [Abstract] [Full Text] [PDF]

				A. J. Chong, T. H. Pohlman, C. R. Hampton, A. Shimamoto, N. Mackman, and E. D. Verrier Tissue factor and thrombin mediate myocardial ischemia-reperfusion injury Ann. Thorac. Surg., February 1, 2003; 75(2): S649 - 655. [Abstract] [Full Text] [PDF]

				K.M.J. Marques and C.A. Visser Myocardial contrast echocardiography in the assessment of pharmacologic intervention of the reperfusion injury Eur. Heart J., January 1, 2003; 24(1): 19 - 20. [Full Text] [PDF]

				Y. Ma, P. Wang, J. F. Kuebler, I. H. Chaudry, and J. L. Messina Hemorrhage induces the rapid development of hepatic insulin resistance Am J Physiol Gastrointest Liver Physiol, January 1, 2003; 284(1): G107 - G115. [Abstract] [Full Text] [PDF]

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