Published online before print May 16, 2005, doi: 10.1161/CIRCULATIONAHA.104.490979
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Circulation. 2005;111:2596-2604.)
© 2005 American Heart Association, Inc.
Heart Failure |
Ventricular Myocyte Caspases Are Directly Responsible for Endotoxin-Induced Cardiac Dysfunction
From EA 2689, CHRU, and Université de Lille 2, IFR 114 IMPRT (S.L., O.J., R.F., C.C., R.N.); INSERM U459 U524, IMPRT (J.K., P.F., P.M.); Laboratoire de Chimie analytique, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille 2 (J.F.G.); and Département de Physiologie, Faculté de Médecine, Université de Lille 2 (S.L., O.J., R.F., R.N.), Lille, France.
Correspondence to Dr Remi Neviere, Département de Physiologie, Faculté de médecine, 1 place de Verdun, Lille Cedex 59045, France. E-mail rneviere{at}univ-lille2.fr
Received July 8, 2004; revision received January 14, 2005; accepted January 20, 2005.
 |
Abstract |
|---|
 Top Abstract IntroductionMethodsResultsDiscussionReferences |
|---|
Background— Although most of the deleterious effects of sepsis-induced apoptosis have been attributed to increased lymphocyte cell death, caspase activation may directly alter cell function of different organ systems. We postulated that left ventricular (LV) cardiomyocyte caspase activation is directly involved in sepsis-induced heart contractile dysfunction.
Methods and Results— LV cardiomyocytes isolated 4 hours after rat treatment with endotoxin injection (10 mg/kg) displayed major reductions in contractile reserve and myofilament response to Ca2+. Concomitantly, endotoxin also induced increases in LV cardiomyocyte caspase-3, -8, and -9-like activities, which were associated with sarcomeric structure destruction and cleavage of components of the cardiac myofilament. Interestingly, zVAD.fmk treatment of septic rat prevented LV cardiomyocyte contractile dysfunction, reductions in myofilament response to calcium, troponin T cleavage, and sarcomere destruction. Serum (10%) of endotoxin-treated rats induced contractile dysfunction, caspase-3–like activity increase, and troponin T cleavage of naive LV cardiomyocytes. The effects of septic serum were prevented in LV cardiomyocytes isolated from zVAD.fmk- or zDEVD.cmk-treated rats or LV cardiomyocytes preincubated with zVAD.fmk or zDEVD.cmk.
Conclusions— The results show an important relationship between endotoxin-induced caspase activation and reduced contractile reserve and sarcomere disarray at the level of single LV cardiomyocytes.
Key Words: apoptosis • myocardial contraction • myocytes, cardiac • inflammation • shock
|
Introduction |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
In the United States, >500 000 patients per year develop bacterial sepsis, with an estimated rising incidence of
1.5% per year.1,2 Sepsis remains the chief cause of death in intensive care units, with mortality rates between 30% and 70%.2,3 Impaired myocardial contractile function is a well-documented feature that greatly contributes to the mortality associated with this pathological condition.2,4 A large body of evidence4–6 suggests that exposure of animals and humans to endotoxin (lipopolysaccharide) mimics the deleterious effects of bacterial sepsis on myocardial function. Although sepsis is generally viewed as a disease aggravated by an inappropriate inflammatory response to endotoxin, implication of apoptosis processes in the pathogenesis of sepsis has been recently demonstrated.2,7 Most of the deleterious effects of endotoxin-induced apoptosis have been attributed to increased circulating and lymphoid tissue resident lymphocyte cell death.7,8 In these studies, protection induced by apoptosis inhibition involved prevention of bacterial growth, alteration in the immune response, and remote effects of apoptosis inhibitors on different cell types.7,8 Alternatively, it could be questioned whether endotoxin-induced activation of apoptosis pathways may directly induce myocardial dysfunction, which can be reduced by apoptosis inhibitors. Indeed, endotoxin may trigger end-stage apoptosis of myocardial cells, which is associated with heart multiple caspase activation and cytochrome c release from the mitochondria.9–12 Interestingly, in vivo caspase activity blockade by broad-spectrum and effector caspase (ie, caspase-3) inhibitors reduced endotoxin-induced heart caspase-3 activity and myocardial dysfunction.11,13 In these studies, however, indirect effects of caspase inhibitors on the extent of activation of immune cells cannot be ruled out.
The major goal of this study was to investigate the role of effector caspase activation in left ventricular (LV) cardiomyocyte dysfunction induced by endotoxin. First, we confirmed that caspase-3 activation and contractile dysfunction may be described at the level of LV cardiomyocytes isolated from septic rat hearts. Second, we developed an in vitro model to test whether caspase inhibition would prevent LV cardiomyocyte contractile dysfunction induced by septic serum, independently of the effects of caspase inhibitors on immunocompetent cells infiltrating the myocardium. Third, we proposed that endotoxin-induced effector caspase activation is centrally involved in LV cardiomyocyte contractile dysfunction and reduced myofilament response to calcium with major sarcomere disorganization and contractile protein cleavage.
|
Methods |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Animal Preparation
Adult male Sprague-Dawley rats (weight, 250 to 300 g) were anesthetized with pentobarbital sodium (50 mg/kg IP). Treatments were administered intravenously via the dorsal penile vein. Four hours after treatment, rats were used for cardiomyocyte isolation and serum preparation, as previously described.9 The protocol was approved by the Animal and Use Committee of Lille, and the care and handling of the animals were conducted in accordance with our national institutional guidelines.
LV Cardiomyocyte Shortening and [Ca2+]i Measurement
LV cardiomyocytes (viability >85%) were plated (100 000 cells per milliliter) and stored at 37°C until use. For contraction amplitude (IonOptix), cells were placed in a flow chamber (30°C) on the stage of a microscope (E800, Nikon) and electrically stimulated at 1 Hz (voltage adjusted to maximize capture). The fluorescent Ca2+ indicator fluo-3 acetoxymethyl ester (6 µmol/L for 20 minutes, 37°C) (Molecular Probes, Inc) was used to measure intracellular Ca2+ as previously described.14 Fluorescence brightness (excitation 465 to 495 nm; emission 515 to 555 nm) was recorded with the use of a CCD camera (C2400-08). Calibration of [Ca2+]i was performed by the use of ionomycin, butanedione monoxime, and MnCl2 solutions, as previously described.14
Serum and Heart Tumor Necrosis Factor-
Determination
Serum and heart homogenates were prepared for tumor necrosis factor- (TNF-) enzyme-linked immunosorbent assay (Quantikine Murine rat TNF, R&D Systems), as described previously.13
Caspase-3, -8, -9–Like Activity Assay
After incubation in assay buffer A (in mmol/L: HEPES 50, NaCl 100, EDTA 1, dithiothreitol 10, with CHAPS 0.1%, glycerol 10%; in µg/mL: aprotinin 10, leupeptin 10, pepstatin 10; pH 7.32), cells were lysed with a Kontes Glass. Next, 200 µg of proteins was diluted in 200 µL of assay buffer, then either Ac-DEVD-AMC, Ac-IETD-AMC, or Ac-LEHD-AMC (50 µmol/L; Biomol) was added. Sample fluorescence (excitation 380 nm; emission 437 nm) was measured at 2 hours (Spex Fluoromax, Isa-Horiba).
Immunohistochemistry for Active Caspase-3
Frozen sections of heart tissue (8 µm) were used to detect caspase-3 by antibodies recognizing the cleaved active form of the enzyme (BD Pharmingen) according to the manufacturer’s instructions.
Western Blot Studies
After cell lysate (buffer A) electrophoresis, polyclonal anti–caspase-3 antibody (BD Pharmingen) and polyclonal anti–caspase-7 antibody (Cell Signaling Technology) were used. After cell fractioning into mitochondrial and cytosolic compartments and electrophoresis, monoclonal anti–cytochrome c antibody (BD Pharmingen) was used. After heart homogenate preparation, polyclonal anti–Bcl-2 and anti-Bax (Santa Cruz Biotechnology) antibodies were used. After cell lysis with specific lysis buffer for myofilament preparation15 and electrophoresis, monoclonal anti–-actinin, anti–troponin T (Sigma), anti–troponin C (Novocastra Laboratories Ltd), and anti–troponin I (Advanced Immunochemical) antibodies were used.
Mitochondrial Membrane Potential
Mitochondrial membrane potential was measured (FACS Calibur Analytic Flow Cytometer (BD Biosciences) in LV cardiomyocytes with the use of the fluorescent probe JC-1 (1 µmol/L, 30 minutes), which produces green fluorescence in the cytoplasm and red-orange fluorescence when concentrated in respiring mitochondria that have a negative internal potential. Data were analyzed with Windows Multiple Document Interface for Flow Cytometry 2.8 software.
Confocal Microscopy Studies
Ventricular sarcomere organization and caspase-3–like activation were assessed on isolated LV cardiomyocytes and visualized by confocal microscopy (Leica TCS NT, Microsystemes). According to the manufacturer’s instructions, caspase-3–like activation was detected in living cells with the use of carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of caspase-3 (FAM-DEVD-FMK) from CaspaTag Caspase 3/7 in situ assay kit (Chemicon International). Polymerized actin fibers, -actinin, and troponin T were visualized by Texas red–phalloidin (Molecular Probes), mouse monoclonal anti–-actinin, and anti–troponin T (Sigma), respectively. Images were quantified and processed with the use of Leica TCS NT software (PowerScan module).
Experimental Design
In the first series of experiments, LV cardiomyocytes of hearts of control (500 µL IV saline), endotoxin Escherichia coli serotype 055:B5 (Sigma)–treated (10 mg/kg IV in 500 µL saline), zVAD.fmk (Bachem)-treated (3 mg/kg IV in 500 µL saline) control, and zVAD.fmk-treated endotoxin-treated rats were isolated for physiological and biological evaluation. Caspase inhibitors were administered at the same time as endotoxin.
In the second series of experiments, the effects of control and septic serum were studied on naive LV cardiomyocytes incubated with caspase inhibitors (zVAD.fmk or zDEVD.cmk; 100 µmol/L). Alternatively, LV cardiomyocytes were isolated from rats treated with caspase inhibitors (zVAD.fmk or zDEVD.cmk; 3 mg/kg body wt, 4 hours).
In the third series of experiments, the effects of endotoxin 100 and 500 ng/mL were studied on LV cardiomyocyte survival, contractile function, caspase-3–like activity, and troponin T cleavage.
Statistical Analysis
Data represent mean±SEM and were analyzed by ANOVA procedures. When a significant difference was found, we identified specific differences between groups using a sequentially rejective Bonferroni procedure. After application of Bonferroni correction, significance was achieved with P<0.01 for comparisons with control.
|
Results |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Endotoxin Rat Treatment Induces Cardiomyocyte Caspase Activation, Contractile Dysfunction, and Sarcomeric Destruction
Four hours after endotoxin treatment, rats presented with diarrhea, polypnea, and pilo-erection. Endotoxin treatment induced downstream caspase activation in LV cardiomyocytes, which was evidenced as caspase-3–like activity increases (Figure 1A), active caspase-3–like staining increases (Figure 2A), and proteolytic activation of caspase-3 (Figure 2B). Proteolytic caspase-7 activity was not detected in LV cardiomyocytes of control and endotoxin-treated rats (data not shown). Cell viability (trypan blue exclusion) and nuclear condensation (Hoechst 33342 staining, 1 µg/mL) of LV cardiomyocytes isolated from control and endotoxin-treated rats were quantified over time. Cell viability in control and septic LV cardiomyocytes remained up to 80% over time (4, 24, and 96 hours; data not shown). Few condensed nuclei (Hoechst staining) were detected (<5%) in control and septic LV cardiomyocytes at 4 and 24 hours after isolation (data not shown). In LV cardiomyocytes of septic rats, caspase-3 activation was associated with heart and plasma TNF-
level (Figure 1B) and upstream caspase-8-like activity (Figure 1A) increases. Mitochondrial-related factors were investigated as a possible upstream pathway that would regulate caspase-3 activation. In LV cardiomyocytes of septic rats, we observed loss of mitochondrial membrane potential, reduced Bcl-2/Bax protein ratio, cytosolic cytochrome c release (Figure 1C), and caspase-9-like activity increases (Figure 1A).
|
em=437 nm) (n=6 in each group). B, Endotoxin induces serum and heart TNF-
m) compared with control (left panel), cytochrome c release in cytosolic compartment, Bax level increases, and reduction of Bcl-2 level. *P<0.01 vs control; n=6 in each group.
|
To assess sarcomeric integrity, we evaluated sarcomeric unit structure, contractile protein cleavage, and caspase activities in the same population of LV cardiomyocytes. Septic LV cardiomyocytes, in which caspase-3–like activity was increased (Figure 2A), displayed hazy, destroyed, and disorganized sarcomeric unit structure (Figure 2A) and troponin T cleavage (32-kDa fragment) (Figure 2B). -Actinin remained unchanged (Figure 2A, 2B). No significant proteolytic cleavages were detected for troponin I (32 kDa), troponin C (19 kDa), or actin (43kDa) (data not shown).
From the same rat heart, cell shortening was measured. In contrast to control LV cardiomyocytes, fractional cell shortening was severely depressed in septic LV cardiomyocytes (Figure 2C).
zVAD.fmk Prevents Endotoxin-Induced Cardiomyocyte Multiple Caspase Activation and Contractile Dysfunction
zVAD.fmk treatment had no effects on clinical signs of sepsis and endotoxin-induced heart and plasma TNF- level increases (data not shown). zVAD.fmk prevented endotoxin-induced LV cardiomyocyte caspase-3 activation (Figure 3A) and proteolytic procaspase-3 cleavage (data not shown), which were associated with reduced caspase-3–like, death receptor–dependent caspase-8, and mitochondrial-related caspase-9 activities (Figure 3B). zVAD.fmk inhibited sarcomere alterations induced by endotoxin (Figure 3C).
|
Fractional shortening and calcium transient were significantly decreased in LV cardiomyocytes isolated from endotoxin-treated rat hearts compared with control LV cardiomyocytes (Figure 4A, 4B). Reductions in calcium transient in LV cardiomyocytes isolated from endotoxin-treated rat hearts were mainly related to increases in diastolic [Ca2+]i (Figure 4C). Rat treatment with zVAD.fmk prevented changes in cell shortening, calcium transient, and diastolic [Ca2+]i observed in septic LV cardiomyocytes.
|
LV cardiomyocyte contractile reserve was further studied by increasing extracellular [Ca2+]o from 0.5 to 3.0 mmol/L at a constant pacing frequency of 1 Hz. Diastolic length decreased slightly, but there were no differences among the groups (Figure 5A). In control LV cardiomyocytes, fractional cell shortening increased in response to the increased [Ca2+]o (Figure 5B). In contrast to control LV cardiomyocytes, fractional cell shortening was severely depressed in septic LV cardiomyocytes, which was prevented by zVAD.fmk rat treatment (Figure 5B).
|
To assess myofilament response to Ca2+ in single LV cardiomyocytes, a repetitive electric tetanization technique (10 Hz; 10 to 20 s) was used in thapsigargin (2 µmol/L)-treated cells, as previously described.16 In the setting of sarcoplasmic reticulum Ca2+-ATPase inhibition by thapsigargin, this procedure results in reproducible maintained elevation of [Ca2+]i during tetanic shortening, thus allowing assessment of a steady state relation between shortening and [Ca2+]o in intact cells. Compared with control LV cardiomyocytes, the relationship between tetanic elevation of [Ca2+]i and fractional shortening was shifted rightward in septic LV cardiomyocytes, which suggests reductions in myofilament response to Ca2+. Reductions in myofilament response to Ca2+ were largely prevented in septic LV cardiomyocytes isolated from rats treated with zVAD.fmk (Figure 5C).
In Vitro Effects of Serum From Endotoxin-Treated Rats on Cardiomyocyte Contractile Function, Caspase-3–Like Activity, and Troponin T
In vitro serum treatment had no effects on LV cardiomyocyte cell death (trypan blue exclusion and Hoechst 33342 staining; data not shown). Exposure of control LV cardiomyocytes with serum of endotoxin-treated rats induced sustained depression of fractional shortening (Figure 6A) and calcium transient (data not shown) starting at 20 minutes, whereas control serum had no effect. Septic serum induced progressive increases in LV cardiomyocyte caspase-3–like activity (Figure 6B) and troponin T cleavage (Figure 6C), whereas control serum had no effect.
|
Serum from endotoxin-treated rats induced reductions in LV cardiomyocyte shortening (Figure 7A), calcium transient (data not shown), and increases in caspase-3–like activity (data not shown), and troponin T cleavage (Figure 7B). Serum from endotoxin plus zVAD.fmk or zDEVD.cmk had similar effects (data not shown). In contrast, these abnormalities were prevented in LV cardiomyocytes incubated (15 minutes) with caspase inhibitor (zVAD.fmk or zDEVD.cmk; 100 µmol/L) or isolated from caspase inhibitor (zVAD.fmk or zDEVD.cmk; 3 mg/kg body wt)–treated rats (Figure 7A, 7B).
|
Exposure (60 minutes) of LV cardiomyocytes to 100 and 500 ng/mL endotoxin had no effects on cell viability, fractional cell shortening, caspase-3–like activity, and troponin T (Table).
|
|
Discussion |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Myocardial dysfunction encountered in septic shock has been attributed to either the cardiodepressive properties of circulating cytokines10 or the impairment of cardiac regional perfusion, and endotoxin may act as a causative agent in both settings.4–6 The present study suggests the new finding that caspase-3 activation plays an important role in endotoxin-induced cardiomyocyte dysfunction, which may be related to changes in calcium myofilament response, contractile protein cleavage, and sarcomere disorganization.
Caspase activation has been implicated in contractile dysfunction of various models of myocardium injury.15,17,18 In these models, contractile dysfunction was not associated with elevated LV cardiomyocyte end-stage nuclear apoptosis.18 Consistently, we observed that in vivo endotoxin treatment induced LV cardiomyocyte caspase-3 activation, without major terminal apoptosis.13 Both death receptor– and mitochondrial-related factors represent possible upstream pathways that would regulate effector caspase activation. These results are consistent with previous studies showing activation of whole heart multiple caspases in response to sepsis.9,11–13 Along with cardiomyocyte caspase-3 activation, endotoxin treatment induced major reduction of contractile function with troponin T cleavage and sarcomere disarray. Endotoxin also produced alterations in myofilament Ca2+ response,16,19 which may participate in the septic myocardial dysfunction. Importantly, functional and morphological alterations of septic cardiomyocytes were prevented by caspase inhibitor zVAD.fmk, suggesting that caspase-3 activation may underlie, at least part, cardiomyocyte contractile dysfunction and calcium homeostasis perturbations. Beneficial effects of caspase inhibition could not be related to changes in TNF- production because zVAD.fmk did not prevented endotoxin-induced increases in serum and heart TNF- levels. These results are, however, consistent with studies indicating that caspase activation may directly alter myofibrillar structure and calcium pumps and channels.20,21
Next, we evaluated the effects of caspase inhibitors on naive cardiomyocytes exposed to septic serum to test potential changes in host immune response and noncardiomyocyte cells associated with caspase inhibitor treatments.7,8 Consistent with studies in which similar in vitro models were used,4,6 we observed that septic serum induced cardiomyocyte dysfunction in the absence of detectable endotoxin levels. Any direct endotoxin effects on LV myocyte structures and functions were further excluded in our in vitro series of experiments.
Septic sera induced cardiomyocyte caspase-3 activation, troponin T cleavage, and contractile dysfunction, which were prevented by the use, both in vivo and in vitro, of zVAD.fmk, a pan-caspase inhibitor, and zDEVD.cmk, a specific caspase-3–like inhibitor.22,23
In contrast, no beneficial effects on LV myocyte structures and functions were observed when serum obtained from endotoxin- and zVAD.fmk-treated rats was used. This is consistent with either high cell permeability and serum short half-life24,25 or absence of effects on immune response of small peptide caspase inhibitors. Caspase-3–like activation can be directly responsible for septic serum–induced cardiomyocyte contractile dysfunction. This contention is supported by the novel findings that caspase-3 may have a role in regulating cardiac contractility and that its inhibition may be associated with reversible states of depressed contractility.26 In addition, similar results emerged from studies showing that contractile performance and sarcomere disarray of failing ventricular myocytes can be corrected via caspase inhibition.17,18 Many putative caspase cleavage sites in cardiac contractile and structural proteins may be identified through databank research with caspase cleavage motifs. Additionally, exposure of myofibrillar proteins to activated caspase-3 results in -actin, -actinin, troponin T,15 and myosin light chain27 cleavage. In our model of sepsis, cardiomyocytes isolated from endotoxin-treated rats displayed troponin T cleavage and sarcomere disorganization, which were prevented by the broad-spectrum caspase inhibitor zVAD.fmk. Although these observations do not demonstrate a causal link, breakdowns of cardiac myofilament components and sarcomere disorganization induced by caspase activation suggest an important interrelationship between caspase activation and functional reserve in cardiomyocytes.
In summary, the results show an important relationship between effector caspase-3 activation and functional contractile reserve in cardiomyocytes in a rat endotoxin model of sepsis. Targeting caspases in cardiomyocytes may be beneficial not only for cardiomyocyte function but also for myocardial depression of sepsis.
|
Acknowledgments |
|---|
This work was supported by grants from Fondation de l’Avenir pour la recherche médicale appliquée (ET1-306, 2001) and INSERM (Biologie et pathologie des régulations cellulaires). U459 and EA 2689 belong to IFR 114 IMPRT (CHU, COL, INSERM, IRCL, Université Lille 2). S. Lancel is supported by grants from of Ministère de l’éducation nationale, de la recherche, et de la technologie MENRT 3857-2002.
|
Footnotes |
|---|
*These authors contributed equally to this work.
|
References |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
1. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med. 2001; 29: 1303–1310.[CrossRef][Medline] [Order article via Infotrieve]
2. Hotchkiss RS, Karl IE. The pathophysiology and treatment of sepsis. N Engl J Med. 2003; 348: 138–150.
3. Parker MM, Shelhamer JH, Bacharach SL, Green MV, Natanson C, Frederick TM, Damske BA, Parrillo JE. Profound but reversible myocardial depression in patients with septic shock. Ann Intern Med. 1984; 100: 483–490.
4. Kumar A, Haery C, Parrillo JE. Myocardial dysfunction in septic shock. Crit Care Clin. 2000; 16: 251–287.[CrossRef][Medline] [Order article via Infotrieve]
5. Suffredini AF, Fromm RE, Parker MM, Brenner M, Kovacs JA, Wesley RA, Parrillo JE. The cardiovascular response of normal humans to the administration of endotoxin. N Engl J Med. 1989; 321: 280–287.[Abstract]
6. Parrillo JE, Burch C, Shelhamer JH, Parker MM, Natanson C, Schuette W. A circulating myocardial depressant substance in humans with septic shock: septic shock patients with a reduced ejection fraction have a circulating factor that depresses in vitro myocardial cell performance. J Clin Invest. 1985; 76: 1539–1553.[Medline] [Order article via Infotrieve]
7. Hotchkiss RS, Tinsley KW, Swanson PE, Chang KC, Cobb JP, Buchman TG, Korsmeyer SJ, Karl IE. Prevention of lymphocyte cell death in sepsis improves survival in mice. Proc Natl Acad Sci U S A. 1999; 96: 14541–14546.
8. Hotchkiss RS, Chang KC, Swanson PE, Tinsley KW, Hui JJ, Klender P, Xanthoudakis S, Roy S, Black C, Grimm E, Aspiotis R, Han Y, Nicholson DW, Karl IE. Caspase inhibitors improve survival in sepsis: a critical role of the lymphocyte. Nat Immunol. 2000; 1: 496–501.[CrossRef][Medline] [Order article via Infotrieve]
9. McDonald TE, Grinman MN, Carthy CM, Walley KR. Endotoxin infusion in rats induces apoptotic and survival pathways in hearts. Am J Physiol. 2000; 279: H2053–H2061.
10. Meldrum DR. Tumor necrosis factor in the heart. Am J Physiol. 1998; 274: R577–R595.[Medline] [Order article via Infotrieve]
11. Fauvel H, Marchetti P, Chopin C, Formstecher P, Neviere R. Differential effects of caspase inhibitors on endotoxin-induced myocardial dysfunction and heart apoptosis. Am J Physiol. 2001; 280: H1608–H1614.
12. Fauvel H, Marchetti P, Obert G, Joulin O, Chopin C, Formstecher P, Neviere R. Protective effects of cyclosporin A from endotoxin-induced myocardial dysfunction and apoptosis in rats. Am J Respir Crit Care Med. 2002; 165: 449–455.
13. Neviere R, Fauvel H, Chopin C, Formstecher P, Marchetti P. Caspase inhibition prevents cardiac dysfunction and heart apoptosis in a rat model of sepsis. Am J Respir Crit Care Med. 2001; 163: 218–225.
14. Gomez AM, Kerfant BG, Vassort G. Microtubule disruption modulates Ca(2+) signaling in rat cardiac myocytes. Circ Res. 2000; 86: 30–36.
15. Communal C, Sumandea M, de Tombe P, Narula J, Solaro RJ, Hajjar RJ. Functional consequences of caspase activation in cardiac myocytes. Proc Natl Acad Sci U S A. 2002; 99: 6252–6256.
16. Tavernier B, Li JM, El-Omar MM, Lanone S, Yang ZK, Trayer IP, Mebazaa A, Shah AM. Cardiac contractile impairment associated with increased phosphorylation of troponin I in endotoxemic rats. FASEB J. 2001; 15: 294–296.
17. Laugwitz KL, Moretti A, Weig HJ, Gillitzer A, Pinkernell K, Ott T, Pragst I, Stadele C, Seyfarth M, Schomig A, Ungerer M. Blocking caspase-activated apoptosis improves contractility in failing myocardium. Hum Gene Ther. 2001; 12: 2051–2063.[CrossRef][Medline] [Order article via Infotrieve]
18. Ruetten H, Badorff C, Ihling C, Zeiher AM, Dimmeler S. Inhibition of caspase-3 improves contractile recovery of stunned myocardium, independent of apoptosis-inhibitory effects. J Am Coll Cardiol. 2001; 38: 2063–2070.
19. Yasuda S. Lew WY. Lipopolysaccharide depresses cardiac contractility and ß-adrenergic contractile response by decreasing myofilament response to Ca2+ in cardiac myocytes. Circ Res. 1997; 81: 1011–1020.
20. Hirota J, Furuichi T, Mikoshiba K. Inositol 1,4,5-trisphosphate receptor type 1 is a substrate for caspase-3 and is cleaved during apoptosis in a caspase-3-dependent manner. J Biol Chem. 1999; 274: 34433–34437.
21. Schwab BL, Guerini D, Didszun C, Bano D, Ferrando-May E, Fava E, Tam J, Xu D, Xanthoudakis S, Nicholson DW, Carafoli E, Nicotera P. Cleavage of plasma membrane calcium pumps by caspases: a link between apoptosis and necrosis. Cell Death Differ. 2002; 9: 818–831.[CrossRef][Medline] [Order article via Infotrieve]
22. Ekert PG, Silke J, Vaux DL. Caspase inhibitors. Cell Death Differ. 1999; 6: 1081–1086.[CrossRef][Medline] [Order article via Infotrieve]
23. Garcia-Calvo M, Peterson EP, Leiting B, Ruel R, Nicholson DW, Thornberry NA. Inhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem. 1998; 273: 32608–32613.
24. Fletcher DS, Agarwal L, Chapman KT, Chin J, Egger LA, Limjuco G, Luell S, MacIntyre DE, Peterson EP, Thornberry NA, et al. A synthetic inhibitor of interleukin-1 beta converting enzyme prevents endotoxin-induced interleukin-1 beta production in vitro and in vivo. J Interferon Cytokine Res. 1995; 15: 243–248.[Medline] [Order article via Infotrieve]
25. Rodriguez I, Matsuura K, Ody C, Nagata S, Vassalli P. Systemic injection of a tripeptide inhibits the intracellular activation of CPP32-like proteases in vivo and fully protects mice against Fas-mediated fulminant liver destruction and death. J Exp Med. 1996; 184: 2067–2072.
26. Condorelli G, Roncarati R, Ross J Jr, Pisani A, Stassi G, Todaro M, Trocha S, Drusco A, Gu Y, Russo MA, Frati G, Jones SP, Lefer DJ, Napoli C, Croce CM. Heart-targeted overexpression of caspase3 in mice increases infarct size and depresses cardiac function. Proc Natl Acad Sci U S A. 2001; 98: 9977–9982.
27. Moretti A, Weig HJ, Ott T, Seyfarth M, Holthoff HP, Grewe D, Gillitzer A, Bott-Flugel L, Schomig A, Ungerer M, Laugwitz KL. Essential myosin light chain as a target for caspase-3 in failing myocardium. Proc Natl Acad Sci U S A. 2002; 99: 11860–11865.
This article has been cited by other articles:
 |
|
 |
|
  G. S. Supinski, M. P. Murphy, and L. A. Callahan MitoQ administration prevents endotoxin-induced cardiac dysfunction Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2009; 297(4): R1095 - R1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, Y. Li, L. Shan, E Shen, R. Chen, and T. Peng Over-expression of calpastatin inhibits calpain activation and attenuates myocardial dysfunction during endotoxaemia Cardiovasc Res, July 1, 2009; 83(1): 72 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pavoine and F. Pecker Sphingomyelinases: their regulation and roles in cardiovascular pathophysiology Cardiovasc Res, May 1, 2009; 82(2): 175 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Radhakrishnan, I. M. Ayoub, and R. J. Gazmuri Activation of caspase-3 may not contribute to postresuscitation myocardial dysfunction Am J Physiol Heart Circ Physiol, April 1, 2009; 296(4): H1164 - H1174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. d. de Tassigny, A. Berdeaux, R. Souktani, P. Henry, and B. Ghaleh The volume-sensitive chloride channel inhibitors prevent both contractile dysfunction and apoptosis induced by doxorubicin through PI3kinase, Akt and Erk 1/2 Eur J Heart Fail, January 1, 2008; 10(1): 39 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Carlson, D. L. Maass, J. White, P. Sikes, and J. W. Horton Caspase inhibition reduces cardiac myocyte dyshomeostasis and improves cardiac contractile function after major burn injury J Appl Physiol, July 1, 2007; 103(1): 323 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Hua, T. Ha, J. Ma, Y. Li, J. Kelley, X. Gao, I. W. Browder, R. L. Kao, D. L. Williams, and C. Li Protection against Myocardial Ischemia/Reperfusion Injury in TLR4-Deficient Mice Is Mediated through a Phosphoinositide 3-Kinase-Dependent Mechanism J. Immunol., June 1, 2007; 178(11): 7317 - 7324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Carlson, D. L. Maass, D. J. White, J. Tan, and J. W. Horton Antioxidant vitamin therapy alters sepsis-related apoptotic myocardial activity and inflammatory responses Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H2779 - H2789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ha, F. Hua, D. Grant, Y. Xia, J. Ma, X. Gao, J. Kelley, D. L. Williams, J. Kalbfleisch, I. W. Browder, et al. Glucan phosphate attenuates cardiac dysfunction and inhibits cardiac MIF expression and apoptosis in septic mice Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1910 - H1918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Larche, S. Lancel, S. M. Hassoun, R. Favory, B. Decoster, P. Marchetti, C. Chopin, and R. Neviere Inhibition of Mitochondrial Permeability Transition Prevents Sepsis-Induced Myocardial Dysfunction and Mortality J. Am. Coll. Cardiol., July 18, 2006; 48(2): 377 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Supinski and L. A. Callahan Polyethylene Glycol-Superoxide Dismutase Prevents Endotoxin-induced Cardiac Dysfunction Am. J. Respir. Crit. Care Med., June 1, 2006; 173(11): 1240 - 1247. [Abstract] [Full Text] [PDF]
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||