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• Figure - (Microsoft Office Document Imaging File) (9.62 MB). Assays for apoptosis in DOX-induced cardiomyopathy. A, TUNEL assays. Photomicrographs showing TUNEL-positive cells in the myocardium: left panel, a TUNEL-positive cardiomyocyte; right panel, a TUNEL-positive nonmyocyte. Arrows point to TUNEL-positive cells; Bars, 20 micrometers. Graphs show the TUNEL assay-based apoptotic index among cardiomyocytes (left) and nonmyocytes (right). B, Western blot analysis of caspase-3 expression. The active (cleaved) form of caspase-3 was not detected in the hearts of any of the groups.