(Circulation. 1996;93:1716-1724.)
© 1996 American Heart Association, Inc.
Articles |
Remodeling Rather Than Neointimal Formation Explains Luminal Narrowing After Deep Vessel Wall Injury
Insights From a Porcine Coronary (Re)stenosis Model
From the Department of Cardiology and Institute of Experimental Clinical Research, Skejby University Hospital, Aarhus, Denmark.
Correspondence to Henning Rud Andersen, MD, PhD, Department of Cardiology, Skejby University Hospital, Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark.
 |
Abstract |
|---|
 Top Abstract IntroductionMethodsResultsDiscussionReferences |
|---|
Background Oversized balloon dilatation of normal porcine coronary arteries usually heals without stenosis formation.
Methods and Results With the purpose of developing a stenotic model and examining the mechanisms of luminal narrowing after angioplasty, we produced a circumferential deep vessel wall injury by inflating and withdrawing an oversized chain-encircled angioplasty balloon in the left anterior descending coronary artery (LAD) of 20 pigs. Three pigs died and did not complete the study. In 8 pigs (group 1), serial coronary arteriography was performed. The lumen diameter (mean±SD) before dilatation was 3.4±0.4 mm; after dilatation, 4.2±0.6 mm; and at follow-ups 2 and 4 weeks later, 1.6±0.4 mm (P<.0001). In 9 pigs (group 2) examined postmortem 3 weeks after dilatation, histology revealed that the injury was deep (out to adventitia) in all arteries and completely circumferential (360°) in all but two arteries. Adventitia was markedly thickened as a result of neoadventitial formation. Injury correlated strongly with neointimal formation (middle LAD, r=.71, P=.00001), but neither injury nor neointima correlated with lumen size (r=.14, P=.46 and r=.34, P=.07, respectively); ie, neointimal formation did not explain late luminal narrowing. Lumen size, however, did correlate strongly with vessel size (r=.74, P=.000005). The late loss in lumen diameter observed angiographically in group 1 substantially exceeded that caused by neointimal formation seen by histology in group 2.
Conclusions The chain-encircled angioplasty balloon produced a circumferential deep vessel wall injury that healed by luminal narrowing. In this porcine model, arterial remodeling was more important than neointimal formation in late luminal narrowing.
Key Words: angioplasty • remodeling • pathology • balloon • stenosis
|
Introduction |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Renarrowing after otherwise successful coronary angioplasty remains a major problem, occurring in 30% to 50% of treated arteries within 3 to 6 months.1 2 3 An injury-related exuberant healing response mediated by SMC proliferation and matrix synthesis (neointimal formation) traditionally has been considered essential in the development of late restenosis. However, antiproliferative agents have not been effective in reducing restenosis in clinical trials1 despite their proven effectiveness in reducing the proliferative response after arterial injury in rats and rabbits.4 The reasons could be that neointimal formation does not, as anticipated, play a critical role in the development of human restenosis and that injury-response processes in smaller animals differ significantly from those in humans.
Contrary to results obtained in rat arterial injury models, those obtained in pig coronary models seem to reflect the pathogenesis of human restenosis much better,4 5 and the pig is now widely used in experimental restenosis studies. The focus of research in these porcine models has also been SMC-dependent neointimal formation.6 7 8 9 10 11 12 Clinical data obtained in human coronary arteries indicate, however, that neointimal formation may not be as important as arterial remodeling in postinterventional restenosis13 14 15 16 ; this phenomenon also has been documented experimentally in rabbit and pig peripheral arteries.17 18 19 Recently, postinterventional remodeling was described in pig coronary arteries, but significant late luminal narrowing did not occur in that model.20 Therefore, we decided to develop a stenotic coronary (re)stenosis model in the pig and describe the processes responsible for the final size of the lumen late after intervention, focusing on both neointimal formation and arterial remodeling. The former was achieved by creating a circumferentially orientated deep vessel wall injury that healed by luminal narrowing; the latter, by serial angiographic examinations and extensive microscopic evaluation of the injured vessels.
|
Methods |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Twenty 6-month-old, 75-kg mixed Danish Landrace–Yorkshire pigs were used in the study. Eight pigs (group 1) from an ongoing survival study underwent serial angiographic examinations to document in vivo changes in lumen size. Nine pigs (group 2) underwent postmortem angiography and histomorphometric examination. Three pigs died and did not complete the study. The pigs were handled according to institutional guidelines and the American Heart Association guidelines for animal research. The pigs were fed a normal nonatherogenic laboratory chow diet.
Chain-Encircled Balloon
A 0.7-mm rough silver chain was mounted on the surface of a
3.5-mm PTCA balloon near its middle. The chain encircled the inflated
balloon and was secured with thin threads fastened to the catheter at
the proximal and distal ends of the balloon (Fig 1
).
Inflated at 8 atm, the total diameter (balloon plus chain) was 
4.9
mm. The diameter of the deflated chain-encircled balloon was 2
mm, and the deflated balloon was introduced into the coronary
artery by a specially constructed guide catheter.
|
In Vitro Experiments
The intention was to produce a deep and completely
circumferential (360°) injury. The reliability of the developed tools
and techniques in producing such an injury was tested in pig hearts
obtained from the local slaughterhouse.
In Vivo Testing
The pigs were sedated with 1000 mg ketamine (Ketalar) IM
and 45 mg midazolam (Dormicum) IM, followed by 500 mg ketamine
IV, intubation, and mechanical ventilation with 6 L/min N2O
and 3 L/min O2. Anesthesia was maintained by
infusion of ketamine and midazolam (400 and 12 mg/h IV,
respectively). Fentanyl (Haldid) 0.5 mg/h was given against pain.
Amiodarone (Cordarone, 900 mg) and ampicillin (Anhypen, 1 g)
were used prophylactically to prevent fatal
arrhythmias and infections, respectively. Before
catheterization, heparin (20 000 U IV) and
acetylsalicylic acid (500 mg IV) were given and
later were supplemented with another bolus of heparin (20 000 U) just
before the PTCA procedure. No further antithrombotic agents were given
at any time until euthanitization.
After exposure of the right common carotid artery, a 14F introducer sheath was inserted for arterial access. A PTCA guide catheter was advanced to the aortic root, and selective coronary angiography was performed. The deflated chain-encircled angioplasty balloon was advanced into the LAD to a position where the diameter of the lumen was 3 mm (usually between the first and second diagonal branches). At that point, the chain, projecting 0.7 mm out from the surface of the inflated 3.5-mm balloon, was supposed to "cut" circumferentially into the vessel wall and "abrade" intima and media when withdrawn. The chain-encircled balloon usually stuck firmly in the LAD when inflated to 8 atm. When the catheter was pulled, the balloon suddenly yielded and moved quickly back through the LAD and out into the aorta, where the balloon was deflated and removed. Frequently, small pieces of tissue were found attached to the chain on the balloon after the procedure. The right carotid artery was ligated, the wound was closed, and the pig was returned to a recovery area.
Serial Coronary Arteriography (Group 1)
Coronary arteriography was performed before dilatation,
2 hours after dilatation, and at follow-up 2 (n=4) and 4 (n=4)
weeks later to document changes in lumen size. The MLD was identified
and measured at follow-up. All angiographic measurements were
determined with end-diastolic frames. For calibration,
a 2.00-mm silver ball was fixed to the tip of a guide wire and
introduced into the distal LAD. The guide wire was withdrawn slowly
during angiography. The angiographic diameter of the silver ball at the
MLD site was used for calibration. Stenosis was calculated as
stenosis=(1-MLD/predilated diameter)x100%.
Postmortem Procedures and Histomorphometric Analysis
(Group 2)
Three weeks after dilatation, the 9 pigs in group 2 were
anesthetized, and after thoracotomy, their hearts were removed
and cannulas were inserted into the ascending aorta. The
coronary arteries were gently flushed with 4%
paraformaldehyde solution (pH 7.2) followed by infusion
of a warm (37°C) barium gelatin mixture at 100 mm Hg. The contrast
medium was hardened by placing the heart overnight in 4%
paraformaldehyde at 4°C; ie, the arteries were fixed
in the distended state. LAD was dissected free of the heart, fixed
further by immersion in 4% paraformaldehyde, and then
placed directly on radiographic films and x-rayed in
different projections. Thereafter, the arteries were cut into cross
sections at 3-mm intervals perpendicular to the direction of blood
flow. All 3-mm segments were processed for microscopic examination. The
final paraffin sections were stained with hematoxylin and eosin and a
trichrome-elastin stain.
Area measurements were performed by point counting with a microscope
(Olympus BHS) equipped with a video camera projecting the field of
vision to a monitor. A computer with a stereological software package
(GRID, Olympus) was connected to the monitor. This software can
superimpose (on the monitor) a test system of regularly spaced points
on the field of vision and defines the area (Ap) each point
(P) represents. By counting the points hitting the area of
interest, the software finds the total area (Atot) by
multiplying the number of hits by the area each point
represents
(Atot=PxAp).21 22 23 Point
counting is a simple and reliable method for area measurements if
enough points are counted. At least 60 points were counted for each
area of interest, giving a coefficient of error <5% according to a
published nomogram.22 The following parameters
were measured (Fig 2).
|
1. Injury score—the part of the circumference measured in degrees
where tunica media had been abraded and adventitia exposed (gap angle;
see Fig 2B
). The sides of the gap angle were drawn from the center of
the lumen, defined as the cross point of two lines drawn perpendicular
to each other, with the lumen divided into four areas of equal size
(determined visually by superimposing a transparent sheet marked with a
cross on a projected image of the vessel). The gap angle was
measured directly with a protractor. An injury score of 360°
indicates a completely circumferential deep injury.
2. Adventitia—area between periadventitial tissues (adipose tissue and myocardium) and external elastic lamina.
3. Vessel size—area circumscribed by the external elastic lamina. The vessel wall is by definition composed of an intimal, a medial, and an adventitial layer. Of note, the vessel size as defined in this study does not include the adventitial component of the vessel wall.
4. Media—area between internal and external elastic lamina (normal media) and, when the internal elastic lamina is missing, areas of remnants of medial tissue (well-organized SMCs with intervening thin elastic fibers).
5. Neointima—area between the lumen and the internal elastic lamina and, when the internal elastic lamina is missing, the area between the lumen and remnants of media tissue or external elastic lamina.
6. Lumen—area circumscribed by the intima/neointima-lumina border.
To evaluate and eliminate the effect of differences in
preinterventional vessel size on the above parameters, LAD
was divided into proximal (cross sections 2 through 6), middle (cross
sections 7 through 11), and distal (cross sections 12 through 16)
1
-cm-long segments.
Postmortem angiographic diameter stenosis was determined as follows: stenosis=(1-MLD/normal distal diameter)x100%.
The distal and not the proximal segment was consistently used as normal reference lumen because LAD proximal to the balloon inflation site was damaged by the withdrawing procedure. Consequently, by use of a smaller lumen as reference, the real stenosis severity was consistently underestimated. If side branches were present in the histological sections, it was not possible to demarcate the areas of interest exactly, and such sections were excluded from analysis.
Statistical Analysis
Data derived from in vivo angiographic examination before
dilatation, after dilatation, and at follow-up were first
analyzed by one-way ANOVA and subsequently by a
two-sided paired t test. Data derived from the study of
all histological sections were pooled according to
segment (proximal, middle, and distal LAD) and analyzed
separately. Linear regression was performed to determine the
correlation between two continuous data (Pearson's correlation test
for independent data). Additionally, the point of maximal
histological luminal narrowing was identified in each
LAD, and mean values of measurements performed here (n=9) were compared
with corresponding mean values of measurements performed 3 mm upstream
and downstream of the narrowest point in each artery (two-sided
paired t test). Data are presented as mean±SD. A
value of P<.05 was considered significant. The
reproducibility of measurements of adventitial area and gap angle was
assessed by double measurements in 20 histological
sections and calculation of the interobserver and intraobserver
variabilities.24
|
Results |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
In Vitro Experiments
Different balloon and chain sizes were tested in 31 coronary arteries postmortem. It was confirmed that a deep and circumferential injury could be produced by inflating and withdrawing a chain-encircled angioplasty balloon within a coronary artery. With this procedure, intima and media were abraded, and adventitia was exposed. To produce a deep and circumferential injury, it was not enough just to inflate the chain-encircled balloon; it was also necessary to move the balloon by pulling the catheter. The pulling procedure created a rather abrupt distal border between normal and abraded media, whereas the proximal border frequently was irregular, with detached medial flaps projecting into the lumen. Three different chain sizes were tested without any obvious differences in circumferential extent or deepness of injury produced, and the thinnest silver chain (diameter, 0.7 mm) was used for the subsequent in vivo experiments.
Coronary Angiography
In vivo coronary angiography (group 1) revealed that the
lumen diameter before dilatation was 3.4±0.4 mm; after dilatation,
4.2±0.6 mm; and at follow-up, 1.6±0.4 mm (P<.0001,
ANOVA; see the Table). All coronary arteries had
an acute gain 2 hours after dilatation, and all arteries had a narrowed
lumen at follow-up (see the Table). The late loss in lumen diameter
was 2.6±0.7 mm. The mean angiographic stenosis was 53%
compared with the vessel diameter before dilatation. Postmortem
coronary angiography (group 2) revealed an MLD of 1.3±0.4 mm,
corresponding to a 32% diameter stenosis compared with the
normal noninjured vascular segment located 13.3±4.3 mm further distal
in LAD (see the Table). In group 2, major side branches departed
between the MLD site and the distal reference segment in six
angiograms. Therefore, the diameter of the distal reference segment
(1.9±0.3 mm; the Table) was smaller than one would expect from
tapering alone. Only eight pigs underwent postmortem angiographic
evaluation because of incomplete contrast injection in one heart.
|
Injury
Histology revealed that the injury was deep (out to adventitia) in
all arteries, but the degree of circumferential injury varied greatly
along the abraded arteries. Seven of the nine arteries contained at
least one segment in which tunica media had been totally removed the
entire circumference around; ie, a 360° circumferential and deep
injury was produced in all but two cases.
Neointima
Like the degree of injury produced, the resultant healing response
varied greatly along the abraded arteries. The newly formed tissue
(neointima) consisted of spindle-shaped cells
surrounded by a rather loose extracellular matrix without elastic
fibers. The cells reacted positively for 
-smooth muscle actin by
immunostaining (Dako 1A4). Neointima filled
medial gaps and usually extended the entire circumference, also
covering apparently normal tunica media and intact internal elastic
lamina. At the base of medial gaps, thrombuslike material rarely was
found. Where the external elastic lamina had been lacerated and within
healed dissections between medial flaps and adventitia, a few small
blood vessels containing radiographic contrast medium were
sometimes seen entering neointima from the adjacent
adventitia (neovascularization), but no capillaries were seen near the
luminal surface. No obvious inflammatory cells were present within
neointima.
Adventitia
Near sites of deep injury, adventitia stained strongly for
collagen and was markedly thickened owing to neoadventitial formation
(Fig 2). Infiltration with mononuclear inflammatory cells was always
recognized. No consistent correlations were found between
adventitial area and injury score, late vessel size, or late lumen
size. The reproducibility of the adventitial measurements revealed
interobserver and intraobserver variabilities of 9% and 8%,
respectively.
Injury-Response Relationships
There was a strong positive correlation between injury score (gap
angle) and resultant neointimal formation for all three
vascular segments; the larger the injury, the more
neointima was apparent 3 weeks later (Fig 3). There was, however, no consistent
relationship between gap angle and maximal neointimal
thickness (proximal LAD, r=.40, P=.12; middle
LAD, r=.64, P=.0001; distal LAD,
r=.26, P=.27). Late vessel size increased with
increasing injury score for proximal and middle LAD segments (Fig 3),
whereas the lumen stayed constant. For distal LAD segments, late vessel
size did not correlate with injury score (r=.003,
P=.99), and the lumen decreased with increasing injury score
(r=-.59, P=.005). Maximal
neointimal thickness (0.77±0.26 mm) and vessel size
(7.05±4.50 mm2) correlated strongly (r=.77,
P=.005) in the 11 histological sections from
all three arterial segments (proximal, middle, and distal)
in the seven arteries in which media had been totally stripped off. The
reproducibility of the gap angle measurements revealed interobserver
and intraobserver variabilities of 1.9% (4°) and 1.7% (2°),
respectively.
|
Neointima, Vessel Size, and Lumen
Neointimal formation and late vessel size correlated
strongly in all three vascular segments; the larger the vessel was 3
weeks after intervention, the more neointima had been
formed (Fig 3). Neointimal formation was expected to
correlate inversely with late lumen size, but it did not (proximal LAD,
r=.10, P=.69; middle LAD, r=.34,
P=.07; and distal LAD, r=-.08,
P=.73). Thus, neointimal formation did not
explain luminal narrowing after deep vessel wall injury. In contrast,
final vessel size correlated strongly with late lumen size in all three
segments. That is, the smaller the vessel was at 3 weeks, the smaller
its lumen was (Fig 3).
Histology of Stenosis
Fig 4 shows all histological
measurements of lumen size, vessel size, and vessel wall components. In
one artery (No. 5), a double lumen was seen in the distal segment owing
to intramural vessel wall dissection. Consequently, this segment of the
artery was excluded from analysis. Compared with normal distal
vascular segments, dilated and abraded segments were enormously
thick-walled. Alignment of the most stenotic segment from
each LAD after the point of maximal stenosis and combination of
all data (mean values, n=9) revealed more clearly the contribution of
individual vessel wall components to vessel size, wall thickness, and
luminal narrowing (Fig 5). At the point of maximal
narrowing, the lumen was significantly smaller than it was 3 mm
distally and 3 mm proximally, despite a similar magnitude of
neointima formation and similar or less tunica media
present. However, the size of the vessel at the point of maximal
luminal narrowing appeared smaller than it was 3 mm distally and 3 mm
proximally. Furthermore, the size of the vessel correlated
significantly with the size of the lumen (r=.79,
P=.02). Thus, late vessel size and not
neointimal formation was the major determinant for luminal
narrowing at the point of maximal stenosis.
|
|
Adventitia was enormously thickened as a result of neoadventitial
formation, but there was no significant correlation between adventitial
area and vessel size at site of maximal stenosis
(r=.44, P=.33). Neoadventitial formation was not
more pronounced at the site of maximal luminal narrowing than it was 3
mm distal and 3 mm proximal to the narrowing (Fig 5). In the normal
reference segment more distal in the LAD, the lumen was 2.3±0.6
mm2, media was 0.8±0.4 mm2,
vessel size was 3.0±0.7 mm2, and adventitia was
1.4±0.7 mm2. Comparisons between maximal stenosis
and the normal reference segment showed that at maximal
stenosis the lumen was significantly smaller (1.4±0.7 versus
2.3±0.6 mm2, P=.002) and adventitia was
significantly larger (7.5±3.7 versus 1.4±0.7 mm2,
P=.009), whereas vessel size was the same (4.1±1.6 versus
3.0±0.7 mm2, P=.07).
|
Discussion |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Dilatation of normal porcine coronary arteries with an oversized balloon usually creates only a single and longitudinally oriented slitlike medial rupture that heals in the course of 2 to 4 weeks, giving rise to an eccentric lesion that hardly protrudes into the lumen; ie, a stenosis rarely develops.6 7 8 20 Oversized stenting was introduced to create a more exuberant healing response that sometimes but not always results in late luminal narrowing.9 10 11 25 By preventing recoil, stenting preserves the acute luminal gain much better than balloon dilatation alone,26 and occasionally the lumen may paradoxically stay larger despite much more stent-induced injury, thrombosis, and proliferation.6 11
New Porcine (Re)stenosis Model
To increase the likelihood of obtaining a stenotic
healing response without stenting (precludes late arterial
remodeling), we maximized the circumferential extension of deep injury
by retracting a chain-encircled balloon in LAD. This concept is
opposite that suggested previously to minimize the healing
response.27 28 Barath et al27 and Bonan et
al28 introduced the "cutting balloon" and
hypothesized that sharp and regular longitudinally oriented vessel wall
incisions might limit the injury-induced healing response and
reduce the risk of restenosis. We hypothesized that doing
the opposite, maximizing the injury by creating an irregular
circumferentially oriented deep vessel wall injury, would induce an
exuberant circumferential healing response and increase the likelihood
of getting the stenotic animal model we wanted. It worked; the
healing response narrowed the lumen, and an angiographic
stenosis developed 2 to 4 weeks later. We decided to evaluate
3-week-old lesions by histology because the neointimal
healing response reportedly is maximal at that time.29
Injury-Response Relationships
The depth of injury caused by oversized stenting10
and the circumferential length of medial rupture caused by oversized
balloon dilatation7 8 20 were previously shown to
correlate with resultant neointimal formation. Also, the
present porcine model revealed a strong
injury-neointima relationship. Of more interest,
neointimal formation and lumen size did not correlate.
Thus, neointimal formation did not influence the most
important outcome parameter: the size of the lumen late
after intervention.
Injury score also correlated with late vessel size. That is, the more a vessel is dilated, the more injury is produced and the more neointima is subsequently formed, but the enlarged vessel can accommodate a huge mass of neointima without luminal narrowing. Only if late luminal loss (includes both neointimal formation and vascular remodeling) exceeds the preceding acute luminal gain does a stenotic lesion develop. In the present porcine model, late luminal narrowing was caused predominantly by constrictive vascular remodeling resulting in a shrunken artery.
Like the degree of injury produced, the resultant healing responses varied greatly along the abraded arteries. The reason for that is not obvious but could be qualitative differences in local cell function that probably are related to the rapidity of complete reendothelialization, the degree of adventitial/perivascular damage, and the magnitude of inflammatory changes.18
Neointima, Remodeling, and
Restenosis
Serial in vivo angiography revealed a late loss in lumen diameter
of 2.6±0.7 mm, giving rise to a late MLD of 1.6±0.4 mm (see the
Table). The mean MLD at 4 weeks' follow-up in group 1 was
identical to that determined by postmortem angiography in group 2 (1.3
mm), indicating that similar results were obtained in the two groups.
Histology revealed a minimal lumen area of 1.4 mm2
surrounded by 1.8-mm2 neointima (Fig 5), which
corresponds to a calculated 0.3-mm-thick ring of
neointima at the point of maximal narrowing. As shown
previously, our histological procedure results in
tissue shrinkage amounting to 20% linear reduction.30
Therefore, the 0.3-mm-thick neointima seen by histology
explains only 0.7 to 0.8 mm (2x0.3 mm+25%) of the 2.6-mm late loss in
lumen diameter seen by in vivo angiography. That is,
neointima accounts for no more than 30% of the late
luminal loss, whereas constrictive vascular remodeling is responsible
for at least 70% of the late loss in this (re)stenosis model.
Compared with the normal predilated lumen size (3.4 mm), the calculated
neointima thickness (0.7 to 0.8 mm) explained only a
fraction of the observed loss in lumen (1.8 mm). Consequently, the
vessel had shrunk, not only from immediately after the procedure to
follow-up but also from before dilatation.
Remodeling is also important for late lumen size in the atherosclerotic
rabbit restenosis model17 18 and some porcine
models,19 20 31 and recent data indicate that remodeling
also may play a very important role in human
restenosis.13 14 15 16 Mintz et al13 14 15 16
used intracoronary ultrasound in humans and found that
remodeling accounted for 65% of the late luminal loss (area). Thus,
our (re)stenosis model mimics the human experience observed by
intracoronary ultrasound. We are currently investigating
the temporal interplay between acute remodeling (overdilatation and
subsequent recoil), late remodeling (chronic enlargement or
contraction), and growth (acute thrombus, neointimal
formation, and neoadventitial formation) in this porcine
(re)stenosis model with angiography, angioscopy,
intracoronary ultrasound, and histology.
Neoadventitia and Restenosis
It was suggested recently that adventitial fibrosis could play an
important role in restenosis by compressing the vessel
(like scar contraction) or preventing compensatory enlargement during
healing after angioplasty.32 We found impressive
neoadventitial formation 3 weeks after intervention, and our data
suggest that one mechanism of restenosis could be
circumferential neoadventitial shrinkage, "strangulating" the
artery to late luminal narrowing.
Remodeling also takes place in native atherosclerotic human arteries and may cause both compensatory vessel enlargement33 and arterial wall shrinkage.34 An important difference between native human atherosclerotic arteries and our injured pig coronary arteries is that severe adventitial damage is usually present in the latter. The adventitial damage in the pig model triggers neoadventitial formation that may be followed by neoadventitial shrinkage. We hypothesize that restenosis in human coronary arteries may be triggered by the same mechanism. During successful angioplasty in humans, the rigid atherosclerotic plaque is separated from the more compliant vessel wall components (plaque-free wall, media, and adventitia) that are stretched, and a tear usually extends deeply into the vessel wall.35 36 The adventitial and perivascular damage caused by stretch and tears may trigger neoadventitial formation and shrinkage (constrictive remodeling), which may cause late luminal narrowing. Some published illustrations of human restenosis do, in fact, reveal adventitial thickening at sites of deep vessel wall injury.37 38
Study Limitations
A major limitation of this and most other porcine
(re)stenosis models is the lack of preexisting vessel wall
disease, like atherosclerosis in humans. This concern
may, however, not be great for neointimal formation after
arterial injury if, as suggested by some postmortem
findings, the reparative response in diseased human coronary
arteries originates from the plaque-free or less diseased vessel
wall rather than the diseased part of the wall.36 Our
porcine model involves tissue removal (medial abrasion) and may mimic
debulking procedures like atherectomy better than balloon angioplasty.
Medial and even adventitial tissue and plaque-free normal vessel
wall are frequently retrieved by atherectomy,39 indicating
that deep and circumferential injury may follow this procedure in
humans. To minimize the influence of differences in predilatation
vessel size in late luminal outcome, we evaluated proximal, middle, and
distal LAD segments separately. However, minor differences in vessel
size must have been present even within individual segments before
dilatation, but they cannot explain the main findings of the
present study. Finally, all data from the same vascular segment
were pooled and analyzed statistically with the assumption of
independence although only nine measurements per segment (one from each
of the nine pigs) were totally independent. The results, however, were
confirmed by the separate analysis of the independent mean
values (Fig 5), indicating that the conclusions drawn are valid.
Conclusions
A porcine coronary (re)stenosis model is described
in which a deep and 360° circumferential injury was produced. The
resultant healing responses narrowed the lumen, and a stenosis
developed, clearly seen angiographically 2 and 4 weeks later. The
stenoses were caused predominantly by constrictive vascular
remodeling rather than neointimal formation, and
adventitial processes could be of importance for the final outcome.
This porcine coronary injury model may prove useful in testing
the effect of treatments not only on postinterventional
neointimal formation but also on arterial
remodeling, stenosis development, and final lumen size—the
most important late outcome parameter.
|
Selected Abbreviations and Acronyms |
|---|
|
|
Acknowledgments |
|---|
The study was supported by grants from Aarhus University Research Foundation, The Danish Heart Foundation, Danish Foundation for the Advancement of Medical Science, and Bristol-Myers Squibb, Denmark. Nycomed DAK donated the acetylsalicylic acid. We want to thank the staff at the Institute of Experimental Clinical Research for good working conditions and Ulrik Baandrup, MD, PhD, Peter Grønning Olesen, MS, and Niels Christian Emmertsen, MS, for assistance with the morphometric analysis and serial angiography examinations.
Received August 16, 1995; revision received October 23, 1995; accepted November 3, 1995.
|
References |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
1. Faxon DP. Restenosis after angioplasty: what have we learned from clinical trials? Cardiol Rev. 1993;1:209-217.
2.
Kuntz RE, Baim DS. Defining coronary
restenosis: newer clinical and angiographic
paradigms. Circulation. 1993;88:1310-1323.
3. Serruys PW, Foley DP, Kirkeeide RL, King SB. Restenosis revisited: insights provided by quantitative coronary angiography. Am Heart J. 1993;126:1243-1267. Editorial. [Medline] [Order article via Infotrieve]
4. Muller DW, Ellis SG, Topol EJ. Experimental models of coronary artery restenosis. J Am Coll Cardiol. 1992;19:418-432. [Abstract]
5.
Ferrell M, Fuster V, Gold HK, Chesebro JH. A
dilemma for the 1990s: choosing appropriate experimental animal model
for the prevention of restenosis.
Circulation. 1992;85:1630-1631.
Editorial.
6. Karas SP, Gravanis MB, Santoian EC, Robinson KA, Anderberg KA, King SB. Coronary intimal proliferation after balloon injury and stenting in swine: an animal model of restenosis. J Am Coll Cardiol. 1992;20:467-474. [Abstract]
7. Bonan R, Paiement P, Scortichini D, Cloutier MJ, Leung TK. Coronary restenosis: evaluation of a restenosis injury index in a swine model. Am Heart J. 1993;126:1334-1340. [Medline] [Order article via Infotrieve]
8. Humphrey WR, Simmons CA, Toombs CF, Shebuski RJ. Induction of neointimal hyperplasia by coronary angioplasty balloon overinflation: comparison of feeder pigs to Yucatan minipigs. Am Heart J. 1994;127:20-31. [Medline] [Order article via Infotrieve]
9.
Schwartz RS, Murphy JG, Edwards WD, Camrud AR,
Vliestra RE, Holmes DR. Restenosis after balloon
angioplasty: a practical proliferative model in porcine
coronary arteries. Circulation. 1990;82:2190-2200.
10. Schwartz RS, Huber KC, Murphy JG, Edwards WD, Camrud AR, Vlietstra RE, Holmes DR. Restenosis and the proportional neointimal response to coronary artery injury: results in a porcine model. J Am Coll Cardiol. 1992;19:267-274. [Abstract]
11. Cox DA, Anderson PG, Roubin GS, Chou CY, Agrawal SK, Cavender JB. Effect of local delivery of heparin and methotrexate on neointimal proliferation in stented procine coronary arteries. Coron Artery Dis. 1992;3:237-248.
12.
Waksman R, Robinson KA, Crocker IR, Gravanis MB,
Cipolla GD, King SB. Endovascular low-dose irradiation
inhibits neointima formation after coronary artery
balloon injury in swine: a possible role for radiation therapy in
restenosis prevention.
Circulation. 1995;91:1533-1539.
13. Mintz GS, Kovach JA, Javier SP, Ditrano CJ, Leon MB. Geometric remodeling is the predominant mechanism of late lumen loss after coronary angioplasty. Circulation. 1993;88(suppl I):I-654. Abstract.
14. Mintz GS, Kovach JA, Pichard AD, Kent KM, Satler LF, Popma JJ, Painter JA, Morgan K, Leon MB. Geometric remodeling is the predominant mechanism of clinical restenosis after coronary angioplasty. J Am Coll Cardiol. 1994;23(suppl A):138A. Abstract.
15. Mintz GS, Matar FA, Kent KM, Pichard AD, Satler LF, Harvey M, Ditrano CJ, Prunka N, Popma JJ, Leon MB. Chronic compensatory arterial dilatation following coronary angioplasty: an intravascular ultrasound study. J Am Coll Cardiol. 1994;23(suppl A):139A. Abstract.
16. Mintz GS, Pichard AD, Kent KM, Satler LF, Popma JJ, Leon MB. Intravascular ultrasound comparison of restenotic and de novo coronary artery narrowings. Am J Cardiol. 1994;74:1278-1280. [Medline] [Order article via Infotrieve]
17.
Kakuta T, Currier JW, Haudenschild CC, Ryan TJ, Faxon
DP. Differences in compensatory vessel enlargement, not intimal
formation, account for restenosis after angioplasty in the
hypercholesterolemic rabbit model.
Circulation. 1994;89:2809-2815.
18.
Lafont A, Guzman LA, Whitlow PL, Goormastic M, Cornhill
JF, Chisolm GM. Restenosis after experimental
angioplasty: intimal, medial, and adventitial changes associated with
constrictive remodeling. Circ Res. 1995;76:996-1002.
19.
Post MJ, Borst C, Kuntz RE. The relative
importance of arterial remodeling compared with intimal
hyperplasia in lumen renarrowing after balloon angioplasty: a study in
the normal rabbit and the hypercholesterolemic
Yucatan micropig. Circulation. 1994;89:2816-2821.
20.
Nunes GL, Sgoutas DS, Redden RA, Sigman SR, Gravanis
MB, King SB, Berk BC. Combination of vitamins C and E alters the
response to coronary balloon injury in the pig.
Arterioscler Thromb Vasc Biol. 1995;15:156-165.
21. Gundersen HJG, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS. 1988;96:379-394. [Medline] [Order article via Infotrieve]
22. Gundersen HJG, Jensen EB. The efficiency of systematic sampling in stereology and its prediction. J Microscopy. 1987;147:229-263. [Medline] [Order article via Infotrieve]
23. Jorgen H, Gundersen G, Boysen M, Reith A. Comparison of semiautomatic digitizer-tablet and simple point counting performance in morphometry. Virchows Arch B Cell Pathol Incl Mol Pathol. 1981;37:317-325. [Medline] [Order article via Infotrieve]
24. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurements. Lancet. 1986:1:307-310.
25.
van der Giessen WJ, Serruys PW, van Beusekom HM, van
Woerkens LJ, van Loon H, Soei LK, Strauss BH, Beatt KJ, Verdouw
PD. Coronary stenting with a new, radiopaque,
balloon-expandable endoprosthesis in pigs.
Circulation. 1991;83:1788-1798.
26. Haude M, Erbel R, Issa H, Meyer J. Quantitative analysis of elastic recoil after balloon angioplasty and after intracoronary implantation of balloon-expandable Palmaz-Schatz stents. J Am Coll Cardiol. 1993;21:26-34. [Abstract]
27. Barath P, Fishbein MC, Vari S, Forrester JS. Cutting balloon: a novel approach to percutaneous angioplasty. Am J Cardiol. 1991;68:1249-1252. [Medline] [Order article via Infotrieve]
28. Bonan R, Joyal M, Untenberg C, Zeiher A, LaBlanche JM, Bertrand M, Specchia G, de Jaegere P, Ruygrok P. Multicentric experience with a cutting balloon for percutaneous transluminal coronary angioplasty: in-hospital results and restenosis rate. Eur Heart J. 1994;15(suppl):335. Abstract.
29. Santoian EC, Gravanis MB, Karas SP, Schneider JE, Anderberg KA, Scott NA, Cipolla G, King SB. Sequence of the reparative phenomena and development of smooth muscle proliferation following coronary angioplasty in a swine restenosis model. Clin Res. 1992;40:364A. Abstract.
30.
Falk E. Coronary artery narrowing
without irreversible myocardial damage or development of collaterals:
assessment of `critical' stenosis in a human model.
Br Heart J. 1982;48:265-271.
31. Brott BC, Labinaz M, Culp SC, Zidar JP, Fortin DF, Virmani R, Phillips HR, Stack RS. Vessel remodeling after angioplasty: comparative anatomic studies. J Am Coll Cardiol. 1994;23(suppl A):138A. Abstract.
32.
Isner JM. Vascular remodeling: honey, I think I
shrunk the artery. Circulation. 1994;89:2937-2941. Editorial.
33. Zarins CK, Weisenberg E, Kolettis G, Stankunavicius R, Glagov S. Differential enlargement of artery segments in response to enlarging atherosclerotic plaques. J Vasc Surg. 1988;7:386-394. [Medline] [Order article via Infotrieve]
34.
Pasterkamp G, Wensing PJW, Post MJ. Hillen B,
Mali WPTM, Borst C. Paradoxical arterial wall
shrinkage may contribute to luminal narrowing of human atherosclerotic
femoral arteries. Circulation. 1995;91:1444-1449.
35. Nobuyoshi M, Kimura T, Ohishi H, Horiuchi H, Nosaka H, Hamasaki N, Yokoi H, Kim K. Restenosis after percutaneous transluminal coronary angioplasty: pathologic observations in 20 patients. J Am Coll Cardiol. 1991;17:433-439. [Abstract]
36. Gravanis MB, Roubin GS. Histopathologic phenomena at the site of percutaneous transluminal coronary angioplasty: the problem of restenosis. Hum Pathol. 1989;20:477-485. [Medline] [Order article via Infotrieve]
37.
Marx J. CMV-p53 interaction may help explain
clogged arteries. Science. 1994;265:320. Editorial.
38.
Essed CE, van den Brand M, Becker AE.
Transluminal coronary angioplasty and early
restenosis: fibrocellular occlusion after wall
laceration. Br Heart J. 1983;49:393-396.
39.
Kuntz R, Hinohara T, Safian R, Selmon MR, Simpson JB,
Baim DS. Restenosis after directional
coronary atherectomy: effects of luminal diameter and deep wall
excision. Circulation. 1992;86:1394-1399.
This article has been cited by other articles:
 |
|
 |
|
  E. Falk, T. Thim, and I. B. Kristensen Atherosclerotic plaque, adventitia, perivascular fat, and carotid imaging. J. Am. Coll. Cardiol. Img., February 1, 2009; 2(2): 183 - 186. [Full Text] [PDF]
|
|
|
|
 |
|
 M Maeng, L O Jensen, E Falk, H R Andersen, and L Thuesen Negative vascular remodelling after implantation of bioabsorbable magnesium alloy stents in porcine coronary arteries: a randomised comparison with bare-metal and sirolimus-eluting stents Heart, February 1, 2009; 95(3): 241 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kawatsu, K. Oda, Y. Saiki, Y. Tabata, and K. Tabayashi External Application of Rapamycin-Eluting Film at Anastomotic Sites Inhibits Neointimal Hyperplasia in a Canine Model Ann. Thorac. Surg., August 1, 2007; 84(2): 560 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Deiner, E. Shagdarsuren, P. L. Schwimmbeck, P. Rosenthal, C. Loddenkemper, U. Rauch, M. Pauschinger, R. Dietz, H.-P. Schultheiss, R. Dechend, et al. Nf-{kappa}b and AP-1 activation is associated with late lumen loss after porcine coronary angioplasty and antiproliferative beta-irradiation Cardiovasc Res, July 1, 2007; 75(1): 195 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K D Krueger, A K Mitra, M G DelCore, W J Hunter III, and D K Agrawal A comparison of stent-induced stenosis in coronary and peripheral arteries J. Clin. Pathol., June 1, 2006; 59(6): 575 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shimokawa and A. Takeshita Rho-Kinase Is an Important Therapeutic Target in Cardiovascular Medicine Arterioscler. Thromb. Vasc. Biol., September 1, 2005; 25(9): 1767 - 1775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wang, X. Zhou, Z. Zhou, N. Mal, L. Fan, M. Zhang, A. M. Lincoff, E. F. Plow, E. J. Topol, and M. S. Penn Platelet, Not Endothelial, P-Selectin Is Required for Neointimal Formation After Vascular Injury Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1584 - 1589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. D. Defawe, R. D. Kenagy, C. Choi, S. Y.C. Wan, C. Deroanne, B. Nusgens, N. Sakalihasan, A. Colige, and A. W. Clowes MMP-9 regulates both positively and negatively collagen gel contraction: A nonproteolytic function of MMP-9 Cardiovasc Res, May 1, 2005; 66(2): 402 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nakayama, M. Amano, A. Katsumi, T. Kaneko, S. Kawabata, M. Takefuji, and K. Kaibuchi Rho-kinase and myosin II activities are required for cell type and environment specific migration Genes Cells, February 1, 2005; 10(2): 107 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Somoza, M. C. Gonzalez, J. M. Gonzalez, F. Abderrahim, S. M. Arribas, and M. S. Fernandez-Alfonso Modulatory role of the adventitia on noradrenaline and angiotensin II responses: Role of endothelium and AT2 receptors Cardiovasc Res, February 1, 2005; 65(2): 478 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Wyttenbach, A. Gallino, M. Alerci, F. Mahler, L. Cozzi, M. Di Valentino, J. J. Badimon, V. Fuster, and R. Corti Effects of Percutaneous Transluminal Angioplasty and Endovascular Brachytherapy on Vascular Remodeling of Human Femoropopliteal Artery by Noninvasive Magnetic Resonance Imaging Circulation, August 31, 2004; 110(9): 1156 - 1161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-H. Chen, L.-Y. Chau, M.-W. Lin, L.-C. Chen, M.-H. Yo, J.-W. Chen, and S.-J. Lin Heme oxygenase-1 gene promotor microsatellite polymorphism is associated with angiographic restenosis after coronary stenting Eur. Heart J., January 1, 2004; 25(1): 39 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Urban, P. Serruys, D. Baumgart, A. Colombo, S. Silber, E. Eeckhout, A. Gershlick, K. Wegscheider, L. Verhees, R. Bonan, et al. A multicentre European registry of intraluminal coronary beta brachytherapy Eur. Heart J., April 1, 2003; 24(7): 604 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S Conte, G. A VanMeter, L. M Akst, T. Clemons, M. Kashgarian, and J. R Bender Endothelial cell seeding influences lesion development following arterial injury in the cholesterol-fed rabbit Cardiovasc Res, February 1, 2002; 53(2): 502 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. M. Hassan, I. M. Lang, M. Ignatescu, R. Ullrich, D. Bonderman, P. Wexberg, F. Weidinger, and H. D. Glogar Increased intimal apoptosis in coronary atherosclerotic vessel segments lacking compensatory enlargement J. Am. Coll. Cardiol., November 1, 2001; 38(5): 1333 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jorgensen, H. Kelbaek, S. Helqvist, G. V. H. Jensen, K. Saunamaki, J. Kastrup, O. Havndrup, H. Bundgaard, J. Kyst Madsen, M. Christiansen, et al. Predictors of coronary in-stent restenosis: importance of angiotensin-converting enzyme gene polymorphism and treatment with angiotensin-converting enzyme inhibitors J. Am. Coll. Cardiol., November 1, 2001; 38(5): 1434 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Ward, P. Kanellakis, D. Ramsey, J. Funder, and A. Bobik Eplerenone Suppresses Constrictive Remodeling and Collagen Accumulation After Angioplasty in Porcine Coronary Arteries Circulation, July 24, 2001; 104(4): 467 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takano, K. Mizuno, K. Okamatsu, S. Yokoyama, T. Ohba, and S. Sakai Mechanical and structural characteristics of vulnerable plaques: analysis by coronary angioscopy and intravascular ultrasound J. Am. Coll. Cardiol., July 1, 2001; 38(1): 99 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shimokawa, K. Morishige, K. Miyata, T. Kandabashi, Y. Eto, I. Ikegaki, T. Asano, K. Kaibuchi, and A. Takeshita Long-term inhibition of Rho-kinase induces a regression of arteriosclerotic coronary lesions in a porcine model in vivo Cardiovasc Res, July 1, 2001; 51(1): 169 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Morishige, H. Shimokawa, Y. Eto, T. Kandabashi, K. Miyata, Y. Matsumoto, M. Hoshijima, K. Kaibuchi, and A. Takeshita Adenovirus-Mediated Transfer of Dominant-Negative Rho-Kinase Induces a Regression of Coronary Arteriosclerosis in Pigs In Vivo Arterioscler. Thromb. Vasc. Biol., April 1, 2001; 21(4): 548 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Schiele, M K Batur, M F Seronde, N Meneveau, P Sewoke, A Bassignot, G Couetdic, F Caulfield, and J-P Bassand Cytomegalovirus, Chlamydia pneumoniae, and Helicobacter pylori IgG antibodies and restenosis after stent implantation: an angiographic and intravascular ultrasound study Heart, March 1, 2001; 85(3): 304 - 311. [Abstract] [Full Text]
|
|
|
|
|
|
J.-G. Bienvenu, J.-F. Tanguay, J.-F. Theoret, A. Kumar, R. G. Schaub, and Y. Merhi Recombinant Soluble P-Selectin Glycoprotein Ligand-1-Ig Reduces Restenosis Through Inhibition of Platelet-Neutrophil Adhesion After Double Angioplasty in Swine Circulation, February 27, 2001; 103(8): 1128 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Godin, E. Ivan, C. Johnson, R. Magid, and Z. S. Galis Remodeling of Carotid Artery Is Associated With Increased Expression of Matrix Metalloproteinases in Mouse Blood Flow Cessation Model Circulation, December 5, 2000; 102(23): 2861 - 2866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Hehrlein, A Kovacs, G.K Wolf, N Yue, and R Nath A novel balloon angioplasty catheter impregnated with beta-particle emitting radioisotopes for vascular brachytherapy to prevent restenosis. First in vivo results Eur. Heart J., December 2, 2000; 21(24): 2056 - 2062. [Abstract] [PDF]
|
|
|
|
 |
|
 E. Sasaki, Y. Tanahashi, Y. Yamasaki, N. Oda, Y. Nozawa, H. Terakawa, K. Miyoshi, Y. Muranaka, H. Miyake, and N. Matsuura Inhibitory Effect of TAS-301, a New Synthesized Constrictive Remodeling Regulator, on Renarrowing after Balloon Overstretch Injury of Porcine Coronary Artery J. Pharmacol. Exp. Ther., December 1, 2000; 295(3): 1043 - 1050. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. F. Drew, H. L. Tucker, K. W. Kombrinck, D. I. Simon, T. H. Bugge, and J. L. Degen Plasminogen Is a Critical Determinant of Vascular Remodeling in Mice Circ. Res., July 21, 2000; 87(2): 133 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mach Toward New Therapeutic Strategies Against Neointimal Formation in Restenosis Arterioscler. Thromb. Vasc. Biol., July 1, 2000; 20(7): 1699 - 1700. [Full Text] [PDF]
|
|
|
|
|
|
R. Virmani, F. D. Kolodgie, A. P. Burke, A. Farb, and S. M. Schwartz Lessons From Sudden Coronary Death : A Comprehensive Morphological Classification Scheme for Atherosclerotic Lesions Arterioscler. Thromb. Vasc. Biol., May 1, 2000; 20(5): 1262 - 1275. [Full Text] [PDF]
|
|
|
|
|
|
S. Ishiwata, S. Verheye, K. A. Robinson, M. Y. Salame, H. de Leon, S. B. King III, and N. A. F. Chronos Inhibition of neointima formation by tranilast in pig coronary arteries after balloon angioplasty and stent implantation J. Am. Coll. Cardiol., April 1, 2000; 35(5): 1331 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Schulz, R A Herrmann, C Beilharz, J Pasquantonio, and E Alt Coronary stent symmetry and vascular injury determine experimental restenosis Heart, April 1, 2000; 83(4): 462 - 467. [Abstract] [Full Text]
|
|
|
|
|
|
K. Morishige, H. Shimokawa, T. Yamawaki, K. Miyata, Y. Eto, T. Kandabashi, K. Yogo, T. Higo, K. Egashira, H. Ueno, et al. Local adenovirus-mediated transfer of C-type natriuretic peptide suppresses vascular remodeling in porcine coronary arteries in vivo J. Am. Coll. Cardiol., March 15, 2000; 35(4): 1040 - 1047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Jorgensen, S. Simonsen, K. Endresen, K. Forfang, K. Vatne, J. Hansen, J. Webb, C. Buller, G. Goulet, J. Erikssen, et al. Restenosis and clinical outcome in patients treated with amlodipine after angioplasty: results from the coronary AngioPlasty Amlodipine REStenosis Study (CAPARES) J. Am. Coll. Cardiol., March 1, 2000; 35(3): 592 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pasterkamp, D. P.V de Kleijn, and C. Borst Arterial remodeling in atherosclerosis, restenosis and after alteration of blood flow: potential mechanisms and clinical implications Cardiovasc Res, March 1, 2000; 45(4): 843 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P Jenkins, G. A Buonaccorsi, R. Mansfield, C. C.R Bishop, S. G Bown, and J. R McEwan Reduction in the response to coronary and iliac artery injury with photodynamic therapy using 5-aminolaevulinic acid Cardiovasc Res, January 14, 2000; 45(2): 478 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Strauss and M. Rabinovitch Adventitial Fibroblasts . Defining a Role in Vessel Wall Remodeling Am. J. Respir. Cell Mol. Biol., January 1, 2000; 22(1): 1 - 3. [Full Text]
|
|
|
|
|
|
B Jorgensen, S Simonsen, K Endresen, K Forfang, T Egeland, A.T Hostmark, and E Thaulow Luminal loss and restenosis after coronary angioplasty. The role of lipoproteins and lipids Eur. Heart J., October 1, 1999; 20(19): 1407 - 1414. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Okamura, M. Ohishi, H. Rakugi, T. Katsuya, Y. Yanagitani, S. Takiuchi, Y. Taniyama, K. Moriguchi, H. Ito, Y. Higashino, et al. Pharmacogenetic Analysis of the Effect of Angiotensin-Converting Enzyme Inhibitor on Restenosis After Percutaneous Transluminal Coronary Angioplasty Angiology, October 1, 1999; 50(10): 811 - 822. [Abstract] [PDF]
|
|
|
|
|
|
M. Sabate, P. W. Serruys, W. J. van der Giessen, J. M.R. Ligthart, V. L.M.A. Coen, I. P. Kay, A. L. Gijzel, A. J. Wardeh, A. den Boer, and P. C. Levendag Geometric Vascular Remodeling After Balloon Angioplasty and {beta}-Radiation Therapy : A Three-Dimensional Intravascular Ultrasound Study Circulation, September 14, 1999; 100(11): 1182 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lafont, E. Durand, J. L. Samuel, B. Besse, F. Addad, B. I. Levy, M. Desnos, C. Guerot, and C. M. Boulanger Endothelial Dysfunction and Collagen Accumulation : Two Independent Factors for Restenosis and Constrictive Remodeling After Experimental Angioplasty Circulation, September 7, 1999; 100(10): 1109 - 1115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O. LERMAN, R. S. SCHWARTZ, J. P. GRANDE, P. F. SHEEDY II, and J. C. ROMERO Noninvasive Evaluation of a Novel Swine Model of Renal Artery Stenosis J. Am. Soc. Nephrol., July 1, 1999; 10(7): 1455 - 1465. [Abstract] [Full Text]
|
|
|
|
|
|
C. Rogers, D. Y. Tseng, J. C. Squire, and E. R. Edelman Balloon-Artery Interactions During Stent Placement : A Finite Element Analysis Approach to Pressure, Compliance, and Stent Design as Contributors to Vascular Injury Circ. Res., March 5, 1999; 84(4): 378 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Le Tourneau, E. Van Belle, D. Corseaux, B. Vallet, G. Lebuffe, B. Dupuis, J.-M. Lablanche, E. McFadden, C. Bauters, and M. E. Bertrand Role of nitric oxide in restenosis after experimental balloon angioplasty in the hypercholesterolemic rabbit: effects on neointimal hyperplasia and vascular remodeling J. Am. Coll. Cardiol., March 1, 1999; 33(3): 876 - 882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Pels, M. Labinaz, C. Hoffert, and E. R. O'Brien Adventitial Angiogenesis Early After Coronary Angioplasty : Correlation With Arterial Remodeling Arterioscler. Thromb. Vasc. Biol., February 1, 1999; 19(2): 229 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R Bennett Apoptosis of vascular smooth muscle cells in vascular remodelling and atherosclerotic plaque rupture Cardiovasc Res, February 1, 1999; 41(2): 361 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Labinaz, K. Pels, C. Hoffert, S. Aggarwal, and E. R O'Brien Time course and importance of neoadventitial formation in arterial remodeling following balloon angioplasty of porcine coronary arteries Cardiovasc Res, January 1, 1999; 41(1): 255 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. de Vrey, G. S. Mintz, C. von Birgelen, T. Kimura, M. Noboyoshi, J. J. Popma, P. W. Serruys, and M. B. Leon Serial volumetric (three-dimensional) intravascular ultrasound analysis of restenosis after directional coronary atherectomy J. Am. Coll. Cardiol., December 1, 1998; 32(7): 1874 - 1880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Varenne, S. Pislaru, H. Gillijns, N. Van Pelt, R. D. Gerard, P. Zoldhelyi, F. Van de Werf, D. Collen, and S. P. Janssens Local Adenovirus-Mediated Transfer of Human Endothelial Nitric Oxide Synthase Reduces Luminal Narrowing After Coronary Angioplasty in Pigs Circulation, September 1, 1998; 98(9): 919 - 926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Lansky, G. S. Mintz, J. J. Popma, A. D. Pichard, K. M. Kent, L. F. Satler, D. S. Baim, R. E. Kuntz, C. Simonton, R. M. Bersin, et al. Remodeling after directional coronary atherectomy (with and without adjunct percutaneous transluminal coronary angioplasty): a serial angiographic and intravascular ultrasound analysis from the optimal atherectomy restenosis study J. Am. Coll. Cardiol., August 1, 1998; 32(2): 329 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lafont and D. Faxon Why do animal models of post-angioplasty restenosis sometimes poorly predict the outcome of clinical trials? Cardiovasc Res, July 1, 1998; 39(1): 50 - 59. [Full Text] [PDF]
|
|
|
|
|
|
S. Nikol, S. Esin, S. Nekolla, T. Y. Huehns, J. Schirmer, M. Schwaiger, and B. Hofling Use of Nuclear Magnetic Resonance Imaging Angiography to Follow-Up Arterial Remodeling in an Animal Model Angiology, April 1, 1998; 49(4): 251 - 258. [Abstract] [PDF]
|
|
|
|
|
|
M. B. DeYoung and D. A. Dichek Gene Therapy for Restenosis : Are We Ready? Circ. Res., February 23, 1998; 82(3): 306 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Libby Gene Therapy of Restenosis : Promise and Perils Circ. Res., February 23, 1998; 82(3): 404 - 406. [Full Text] [PDF]
|
|
|
|
|
|
A. Kumar, J. L. Hoover, C. A. Simmons, V. Lindner, and R. J. Shebuski Remodeling and Neointimal Formation in the Carotid Artery of Normal and P-Selectin–Deficient Mice Circulation, December 16, 1997; 96(12): 4333 - 4342. [Abstract] [Full Text]
|
|
|
|
|
|
C. I. Seye, A.-P. Gadeau, D. Daret, F. Dupuch, P. Alzieu, L. Capron, and C. Desgranges Overexpression of the P2Y2 Purinoceptor in Intimal Lesions of the Rat Aorta Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3602 - 3610. [Abstract] [Full Text]
|
|
|
|
|
|
S.S. Srivatsa, L. A Fitzpatrick, P. W Tsao, T. M Reilly, D. R Holmes Jr, R. S Schwartz, and S. A Mousa Selective {alpha}v{beta}3 integrin blockade potently limits neointimal hyperplasia and lumen stenosis following deep coronary arterial stent injury:: Evidence for the functional importance of integrin {alpha}v{beta}3 and osteopontin expression during neointima formation Cardiovasc Res, December 1, 1997; 36(3): 408 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. Kullo, G. Mozes, R. S. Schwartz, P. Gloviczki, T. B. Crotty, D. A. Barber, Z. S. Katusic, and T. O'Brien Adventitial Gene Transfer of Recombinant Endothelial Nitric Oxide Synthase to Rabbit Carotid Arteries Alters Vascular Reactivity Circulation, October 7, 1997; 96(7): 2254 - 2261. [Abstract] [Full Text]
|
|
|
|
|
|
R. Waksman, J. C. Rodriguez, K. A. Robinson, G. D. Cipolla, I. R. Crocker, N. A. Scott, S. B. King III, and J. N. Wilcox Effect of Intravascular Irradiation on Cell Proliferation, Apoptosis, and Vascular Remodeling After Balloon Overstretch Injury of Porcine Coronary Arteries Circulation, September 16, 1997; 96(6): 1944 - 1952. [Abstract] [Full Text]
|
|
|
|
|
|
T. Kimura, S. Kaburagi, T. Tamura, H. Yokoi, Y. Nakagawa, H. Yokoi, N. Hamasaki, H. Nosaka, M. Nobuyoshi, G. S. Mintz, et al. Remodeling of Human Coronary Arteries Undergoing Coronary Angioplasty or Atherectomy Circulation, July 15, 1997; 96(2): 475 - 483. [Abstract] [Full Text]
|
|
|
|
|
|
V. Verin, P. Urban, Y. Popowski, M. Schwager, P. Nouet, P. A. Dorsaz, P. Chatelain, J. M. Kurtz, and W. Rutishauser Feasibility of Intracoronary ß-Irradiation to Reduce Restenosis After Balloon Angioplasty: A Clinical Pilot Study Circulation, March 4, 1997; 95(5): 1138 - 1144. [Abstract] [Full Text]
|
|
|
|
|
|
A. Zalewski and Y. Shi Vascular Myofibroblasts : Lessons From Coronary Repair and Remodeling Arterioscler. Thromb. Vasc. Biol., March 1, 1997; 17(3): 417 - 422. [Full Text]
|
|
|
|
|
|
T. T. Lim, D. H. Liang, J. Botas, J. S. Schroeder, S. N. Oesterle, and A. C. Yeung Role of Compensatory Enlargement and Shrinkage in Transplant Coronary Artery Disease: Serial Intravascular Ultrasound Study Circulation, February 18, 1997; 95(4): 855 - 859. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Shi, J. E. O'Brien, L. Ala-Kokko, W. Chung, J. D. Mannion, and A. Zalewski Origin of Extracellular Matrix Synthesis During Coronary Repair Circulation, February 18, 1997; 95(4): 997 - 1006. [Abstract] [Full Text]
|
|
|
|
|
|
D. B. Schneider and D. A. Dichek Intravascular Stent Endothelialization: A Goal Worth Pursuing? Circulation, January 21, 1997; 95(2): 308 - 310. [Full Text]
|
|
|
|
|
|
Y. Shi, J. E. O'Brien, A. Fard, J. D. Mannion, D. Wang, and A. Zalewski Adventitial Myofibroblasts Contribute to Neointimal Formation in Injured Porcine Coronary Arteries Circulation, October 1, 1996; 94(7): 1655 - 1664. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. A. J. Schulze-Bauer, P. Regitnig, and G. A. Holzapfel Mechanics of the human femoral adventitia including the high-pressure response Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2427 - H2440. [Abstract] [Full Text] [PDF]
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||