Files in this Data Supplement:

• Supplemental Methods - (Microsoft Word) (46.5 kb). Includes Supplemental Table.
• Figure I - (Microsoft PowerPoint) (80.0 kb). Real-time PCR experiments using SYBR green and β₁ TaqMan probe. A, Melting curve analysis showing a single sharp peak at 79.6 °C for β₁ amplification using SYBR green with the oligonucleotides described in the Supplementary Table. Note the lack of amplification in the absence of reverse-transcribed (RT) product. B, Agarose gel showing single band amplifications with the expected sizes (83 and 78 bp for β₁ and α, respectively). C, Comparison of the levels of β₁ mRNA expression in A7r5 cells maintained in normoxia (20% O₂) or hypoxia (1% O₂) for 24 h measured with SYBR green and a β₁-specific TaqMan probe. Bars show the percentage of β₁ transcript in hypoxia versus normoxia (mean±SEM; n=5 in the two sets of experiments). Note that the effect of hypoxia on β₁ mRNA is the same with both amplification methods.
• Figure II - (Microsoft PowerPoint) (101 kb). Contractive responses to phenylephrine (Phe, 10 μmol/L) of aortic rings subjected to different equilibration times. A, Superposition of recordings from two representative experiments using either 60 (continuous line) or 100 (dotted line) min equilibration times before application of Phe. Note that the effects of Phe (before and after exposure to 100 nmol/L iberiotoxin) are the same with both equilibration times. B, Quantification of Phe effects in arterial rings (n=8) maintained at the resting tension for 60 (filled symbols) and 100 (open symbols) min. C, Same data as in B (at 1 min after Phe application) expressed as percentage of resting tension. Data are given as mean±SEM.