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• Supplemental Methods - (Word) (68.5 KB)
• Figure - (Power Point) (49.5 KB) Recovering of depressed cardiac function and calcium transients of KO cardiac myocytes after adenoviral SERCA2a expression. A) Superimposed tracings of averaged normalized cytosolic Ca²⁺ transients in different cardiac myocytes isolated from Hspa 1a-/- /Hspa 1b-/- null mice (KO) 48 h after infection with an empty adenovirus (Adv.Ctr) or an adenovirus expressing SERCA2a (Adv.SERCA). Myocytes were electrically paced at 0.3 Hz. B) Cumulative analyses of calcium transients showing duration of intracellular calcium decline to 50% in wild type (WT) KO+Adv.Ctr and KO+Adv.SERCA treated cells. C) Cumulative analysis of fractional cell shortening (%), rate of contraction and rate of relaxation (D). (*P<0.05 vs. WT+Adv-Ctr; †P<0.05 vs. KO+Adv-Ctr. n = 30 cells for each group).