Files in this Data Supplement:

• Supplemental Methods - (Word) (65.5 KB)
• Table - (Word) (30.5 KB)
• Figure I - (Power Point) (16.5 KB) Quantitative PCR analysis of genomic copies of inserted transgene. To quantify the number of transgenes inserted into the genome, 40ng of genomic DNA from wild-type, A₁-TG line B and A₁-TG line C mice were used in real-time PCR reaction with a primer set that is specific for both human and mouse A₁-AR. Each experimental group was performed in triplicate and repeated 3 times. Data are presented as relative fold changes to the endogenous mouse A₁-AR gene.
• Figure II - (Power Point) (15.5 KB) Quantitative PCR analysis of A₁-AR transgene expression. Total RNA was extracted from the biventricular tissues of wild-type, A₁-TG line B and A₁-TG line C mice. Ten μg total RNA was used to synthesize double-stranded cDNA and were then used in real-time PCR reaction with primers specific for A₁-AR and for actin. Each experimental group was performed in triplicate and repeated three times. Each experimental group was performed in triplicate. The ΔCT method was used to quantify the results, which are presented as relative fold changes to the actin gene.
• Figure III - (Power Point) (14.5 KB) Ventricular weight/body weight ratio (VW/BW) of wild type mice (WT), mice constitutively expressing tTA transactivating factor (tTA) and wild type mice on 300mg/kg doxycycline diets (DOX). Seven to ten 12-weeks old mice from each group were measured. No significance was detected at P value setting of P<0.05.
• Figure IV - (Power Point) (14.5 KB) Fractional shortening (FS) in wild type mice (WT), mice constitutively expressing tTA transactivating factor (tTA) and wild type mice on 300mg/kg doxycycline diets (DOX) inhibition of A1-AR expression in. Echocardiography was performed on 7-10 twelve-week-old male mice. No significance was detected at P value setting of P<0.05.
• Figure V - (Power Point) (123 KB) Kinase phosphorylation in A₁-AR expressing myocardium. Ventricular extracts from 6-week-old male mice with indicated transgenes were probed with antibodies against phospho-Ser473 Akt, phospho-JNK, phospho-P38, phospho-ERK, and actin. Data represent 1 of 3 independent experiments.
• Figure VI - (Power Point) (19 KB) Amino acid conservation between human and mouse A₁-AR.
• Figure VII - DOX treatment reversed cardiac phenotype in A₁-TG_Con mice. Horizontal slice of myocardium and Picro-sirius Red staining from 12-weeks old mice are shown.