Files in this Data Supplement:

• Supplemental Methods - (Microsoft Word) (38.5 kb)
• Figure I - (Power Point) (89.5 kb) Chimerism of GFP⁺ bone marrow-translanted mice. BMT was performed from GFP-transgenic mice to wild-type mice. The frequency of GFP⁺ cells in peripheral nucleated cells at 6 weeks after BMT was determined by using flow cytometry. (A) wild-type mice (negative control). (B) GFP⁺ bone marrow transplanted mice.
• Figure II - (Power Point) (655 kb) Reendothelialization at 21 days after vascular injury. Wire-mediated vascular injury was produced in the wild-type and ASC^¡V/¡V mice. The femoral arteries were excised at 21 days after injury. Immunohistochemical staining for endothelial cells (CD31 and VE-cadherin) was performed. Bar represents 100 μm.
• Figure III - (Power Point) (2.56 MB) Expression of activated caspase-3 in the vascular wall after vascular injury. Wire-mediated vascular injury was produced in the wild-type and ASC^¡V/¡V mice. The femoral arteries were excised at 6 h after injury. Immunohistochemical staining for activated caspase-3 was performed. Rabbit IgG was used as a negative control. Bar represents 50 μm.
• Figure IV - (Power Point) (355 kb) Detection of bone marrow-derived cells in the neointimal lesion. Wire-mediated vascular injury was produced in BMT mice (C57BL/6) whose bone marrow was replaced with that of GFP-transgenic mice. The femoral arteries were excised at 21 days after injury. Double immunofluorescence staining for GFP (green) and αSMA (red) was performed. Nucleic acid was stained with DAPI (blue). Arrows and arrowheads indicate GFP⁺/αSMA⁺ cells and GFP⁺/αSMA^- cells, respectively. Representative photographs are shown (n=3).