Files in this Data Supplement:

• Methods - (Microsoft Word file) (50 KB).
• Figure I - (Microsoft PowerPoint file) (5.7 MB). Bright-field images of primary-passage cardiac fibroblasts near confluence derived from (A) left-ventricular free wall (LVFW), (B) left-atrial appendage (LAA) and (C) left-atrial free wall (LAA, original magnification x10, bar=70 µm). D: Upper panel: αSMA and GAPDH bands from two LVFW/LAA/LAFW groups (LVFW1, LAA1, LAFW1 and LVFW2, LAA2, LAFW2). Lower panel: mean±SEM αSMA band-intensities relative to GAPDH for n=6 groups, **P<0.01 vs. LVFW. E: 3HThymidine incorporation (mean±SEM) in first-passage LVFW, LAA and LAFW fibroblasts treated with 7%-FBS. Dashed horizontal line=baseline (non-FBS exposed cells). n=6, ***P<0.001 vs. LVFW
• Figure II - (Microsoft PowerPoint file) (755 KB). Ventricular (A) and atrial (B) fibroblasts in culture with immunofluorescent labeling for αSMA (in red) and vimentin (in green) (original magnification x63, bar = 20 μm).
• Figure III - (Microsoft PowerPoint file) (1.5 MB). Immunofluorescently-labeled confocal images from 1 representative dog per group (without phase contrast in merge, original magnification x63, bar = 20 μm). αSMA staining is depicted in red, with vimentin in green.
• Figure IV - (Microsoft PowerPoint file) (143 KB). Sample of the dual parameter dot plots used for flow cytometry gating (A) and histogram analysis of DNA content in propridium iodide-stained cells (B).
• Table I - (Microsoft Excel file) (33 KB). All genes found to be differentially expressed in first passage atrial and ventricular fibroblasts grown to confluence in 7% FBS, n=5 studied pair-wise for each animal.
• Table II - (Microsoft Excel file) (169 KB). Normalized RMA values (log₂, absolute and absolute At/Vent fold change) for all probesets of genes for which at least one probeset was statistically significantly different among atrial and ventricular fibroblasts.