(Circulation. 2000;102:1020.)
© 2000 American Heart Association, Inc.
Clinical Investigation and Reports |
Red Wine Intake Prevents Nuclear Factor-
B Activation in Peripheral Blood Mononuclear Cells of Healthy Volunteers During Postprandial Lipemia
From the Research Laboratory, Instituto de Investigación Médica, Fundación Jiménez Díaz, Universidad Autónoma de Madrid (L.M.B.-C., C.B., M.O., M.A.H.-P., P.C., J.G.-G., J.E.), and the Lipid Research Unit, Hospital Gregorio Marañón (M.V., L.A.A.-S., J.M.), Madrid, Spain.
Correspondence to Professor Jesús Egido, Renal and Vascular Laboratory, Fundación Jiménez Díaz, Avenida Reyes Católicos 2, 28040 Madrid, Spain. E-mail evasc{at}fjd.es
Background—Several
epidemiological studies have demonstrated the beneficial effect of red
wine intake in reducing total and cardiovascular
mortality. This effect has been attributed in part to its antioxidant
properties. Because the monocytes/macrophages and the nuclear
transcription factor 
B (NF-B) are implicated in the pathogenesis
of atherosclerotic lesions, we examined the effect of red wine intake
on the activation of NF-B in peripheral blood
mononuclear cells.
Methods and Results—Sixteen healthy volunteers were studied 3
times each: after a moderate dose, a low dose, and no wine with a
fat-enriched breakfast. Lipid profile and NF-B activation
(electrophoretic mobility shift assay) were examined in blood samples
taken before and 3, 6, and 9 hours after wine intake. In addition,
mononuclear cells were incubated with VLDL in the presence of some
antioxidants (quercetin and 
-tocopherol succinate)
contained in red wine to study their effects on NF-B activation.
Subjects receiving a fat-enriched breakfast had increased NF-B
activation in peripheral blood mononuclear cells coinciding
with the augmentation in total triglycerides and
chylomicrons. Red wine intake prevented NF-B activity even though it
induced a certain increase in serum lipids, particularly VLDL, that did
not increase after the fat ingestion alone. However, another form of
alcohol intake (vodka) did not modify the NF-B activation provided
by postprandial lipemia. In cultured mononuclear cells, isolated human
VLDL caused NF-B activation in a time-dependent manner that did not
occur in the presence of the red wine antioxidants quercetin and
-tocopherol.
Conclusions—Our results provide a new potential mechanism to explain the beneficial effects of red wine intake in the reduction of cardiovascular mortality.
Key Words: antioxidants • blood cells • molecular biology • atherosclerosis
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