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(Circulation. 1971;43:I-124.)
© 1971 American Heart Association, Inc.

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Effects of a Hyperosmotic Perfusate on Extended Preservation of the Heart

SIDNEY LEVITSKY M.D.¹; EDWARD D. MULLIN M.D.¹; ROBERT E. SLOANE M.D.¹; VICTOR J. FERRANS M.D., PH.D.¹; L. MAXIMILIAN BUJA M.D.¹; WILLIS H. WILLIAMS M.D.¹; ANDREW G. MORROW M.D.¹

¹ From the Clinic of Surgery and the Section of Pathology, National Heart and Lung Institute, Bethesda, Maryland.

Twelve canine hearts were preserved for 18 hours with filtered plasma, with and without added dextran, passed through a Kolobow membrane oxygenator at 8 to 10 C. Active and passive length-tension curves (LTC) were obtained with an isovolumetric balloon after rewarming to 37 C with blood pumped from a support animal. Nine additional control hearts were similarly evaluated immediately after removal. The active LTC was unchanged for all three groups. Diastolic compliance was moderately decreased in all the perfused hearts. Interstitial edema was present in varying degrees in all hearts preserved with filtered plasma without dextran but was absent in the dextran-plasma group. These data indicate the advantages of using a hyperosmotic perfusate for extended cardiac preservation.

Key Words: Dextran-plasma • Isolated heart preparation • Diastolic compliance • Ultrastructural studies