(Circulation. 1995;92:1230-1235.)
© 1995 American Heart Association, Inc.
Articles |
Smooth Muscle Cell Proliferation Is Proportional to the Degree of Balloon Injury in a Rat Model of Angioplasty
From the Division of Cardiology, Department of Medicine & Molecular and Cellular Pathology, Federico II University, Naples, and the Department of Experimental and Clinical Medicine, Facoltà di Medicina di Catanzaro, Italy.
Correspondence to Ciro Indolfi, MD, FACC, Division of Cardiology, Federico II University, Via Pansini, 5 80131 Napoli, Italy.
Background A variable degree of smooth muscle cell (SMC) proliferation after balloon injury has been reported in previous rat studies. It is unknown whether balloon injury induces c-fos expression and whether it is related to the degree of vascular injury in vivo. Therefore, we tested the hypothesis that proportional increases in neointimal formation and c-fos expression might be present after different degrees of balloon dilation.
Methods and Results Angioplasty of the carotid artery was performed with a balloon catheter. Vascular injury was evaluated at 0, 0.5, 1.0, 1.5, and 2 atm (n=6 for all). In 40 additional rats, total RNA dot blots were performed to assess the effect of various degrees of balloon injury on c-fos expression. SMC proliferation proportional to the increases of inflation pressure was found between 0 and 2 atm with neointimal areas of 0.002±0.002, 0.069±0.014, 0.128±0.043, 0.190±0.010, and 0.255±0.041 mm2, respectively. When the degree of SMC proliferation (neointima and neointima/media ratio) was plotted against balloon inflation pressure, a linear relation was observed (r=.733, P<.001 and r=.755, P<.001, respectively). An increase in c-fos expression proportional to the degree of injury was found 30 minutes after injury.
Conclusions Neointimal proliferation produced by balloon injury is related to balloon inflation pressure, supporting the concept of an SMC proliferative response proportional to the degree of injury. The increase in SMC proliferation is associated with a proportional increase in the early expression of the c-fos nuclear proto-oncogene.
Key Words: muscle, smooth • stenosis • angioplasty
This article has been cited by other articles:
 |
|
 |
|
  V. Mollace, S. Ragusa, I. Sacco, C. Muscoli, F. Sculco, V. Visalli, E. Palma, S. Muscoli, L. Mondello, P. Dugo, et al. The Protective Effect of Bergamot Oil Extract on Lecitine-like OxyLDL Receptor-1 Expression in Balloon Injury-related Neointima Formation Journal of Cardiovascular Pharmacology and Therapeutics, June 1, 2008; 13(2): 120 - 129. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Kariya, N. Tanigawa, H. Kojima, A. Komemushi, Y. Shomura, T. Shiraishi, T. Kawanaka, and S. Sawada Primary Patency with Cutting and Conventional Balloon Angioplasty for Different Types of Hemodialysis Access Stenosis Radiology, May 1, 2007; 243(2): 578 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Muscoli, I. Sacco, W. Alecce, E. Palma, R. Nistico, N. Costa, F. Clementi, D. Rotiroti, F. Romeo, D. Salvemini, et al. The Protective Effect of Superoxide Dismutase Mimetic M40401 on Balloon Injury-Related Neointima Formation: Role of the Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 44 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rius, J. Martinez-Gonzalez, J. Crespo, and L. Badimon Involvement of Neuron-Derived Orphan Receptor-1 (NOR-1) in LDL-Induced Mitogenic Stimulus in Vascular Smooth Muscle Cells: Role of CREB Arterioscler Thromb Vasc Biol, April 1, 2004; 24(4): 697 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Curcio, D. Torella, G. Cuda, C. Coppola, M. C. Faniello, F. Achille, V. G. Russo, M. Chiariello, and C. Indolfi Effect of stent coating alone on in vitro vascular smooth muscle cell proliferation and apoptosis Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H902 - H908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ward, C. Indolfi, and D. Torella Physical Training and Restenosis * Response Circ. Res., April 4, 2003; 92 (6): e60 - e60. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Takeuchi, K. Ohmori, I. Kondo, A. Oshita, K. Shinomiya, Y. Yu, Y. Takagi, K. Mizushige, K. Kangawa, and M. Kohno Potentiation of C-type natriuretic peptide with ultrasound and microbubbles to prevent neointimal formation after vascular injury in rats Cardiovasc Res, April 1, 2003; 58(1): 231 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Martinez-Gonzalez, J. Rius, A. Castello, C. Cases-Langhoff, and L. Badimon Neuron-Derived Orphan Receptor-1 (NOR-1) Modulates Vascular Smooth Muscle Cell Proliferation Circ. Res., January 10, 2003; 92(1): 96 - 103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Indolfi, D. Torella, C. Coppola, A. Curcio, F. Rodriguez, A. Bilancio, A. Leccia, O. Arcucci, M. Falco, D. Leosco, et al. Physical Training Increases eNOS Vascular Expression and Activity and Reduces Restenosis After Balloon Angioplasty or Arterial Stenting in Rats Circ. Res., December 13, 2002; 91(12): 1190 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Indolfi, D. Torella, C. Coppola, E. Stabile, G. Esposito, A. Curcio, A. Pisani, L. Cavuto, O. Arcucci, M. Cireddu, et al. Rat carotid artery dilation by PTCA balloon catheter induces neointima formation in presence of IEL rupture Am J Physiol Heart Circ Physiol, August 1, 2002; 283(2): H760 - H767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Indolfi Genetic factors in atherosclerosis: status and perspectives Eur. Heart J. Suppl., March 1, 2002; 4(suppl_B): B14 - B16. [Abstract] [PDF]
|
|
|
|
|
|
P. Maffia, A. Ianaro, R. Sorrentino, L. Lippolis, F. M. Maiello, P. del Soldato, A. Ialenti, and G. Cirino Beneficial Effects of NO-Releasing Derivative of Flurbiprofen (HCT-1026) in Rat Model of Vascular Injury and Restenosis Arterioscler Thromb Vasc Biol, February 1, 2002; 22(2): 263 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Indolfi, D. Torella, L. Cavuto, A. M. Davalli, C. Coppola, G. Esposito, M. V. Carriero, A. Rapacciuolo, E. Di Lorenzo, E. Stabile, et al. Effects of Balloon Injury on Neointimal Hyperplasia in Streptozotocin-Induced Diabetes and in Hyperinsulinemic Nondiabetic Pancreatic Islet-Transplanted Rats Circulation, June 19, 2001; 103(24): 2980 - 2986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kobayashi, Y. Honda, L. G. Christie, P. S. Teirstein, S. R. Bailey, C. L. Brown III, R. V. Matthews, A. C. De Franco, R. S. Schwartz, S. Goldberg, et al. Long-term vessel response to a self-expanding coronary stent: a serial volumetric intravascular ultrasound analysis from the ASSURE trial J. Am. Coll. Cardiol., April 1, 2001; 37(5): 1329 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Koshy, A. Self, P. J Kadowitz, V. A Fonseca, and D. B McNamara Effects of low-dose candesartan on the rate of re-endothelialisation following vascular wound healing Journal of Renin-Angiotensin-Aldosterone System, March 1, 2001; 2(1_suppl): S81 - S83. [Abstract] [PDF]
|
|
|
|
|
|
C. Indolfi, E. Stabile, C. Coppola, A. Gallo, C. Perrino, G. Allevato, L. Cavuto, D. Torella, E. Di Lorenzo, G. Troncone, et al. Membrane-Bound Protein Kinase A Inhibits Smooth Muscle Cell Proliferation In Vitro and In Vivo by Amplifying cAMP-Protein Kinase A Signals Circ. Res., February 16, 2001; 88(3): 319 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Indolfi, E. Di Lorenzo, A. Rapacciuolo, A. M. Stingone, E. Stabile, A. Leccia, D. Torella, R. Caputo, F. Ciardiello, G. Tortora, et al. 8-Chloro-cAMP inhibits smooth muscle cell proliferation in vitro and neointima formation induced by balloon injury in vivo J. Am. Coll. Cardiol., July 1, 2000; 36(1): 288 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Autieri, C. Carbone, and A. Mu Expression of Allograft Inflammatory Factor-1 Is a Marker of Activated Human Vascular Smooth Muscle Cells and Arterial Injury Arterioscler Thromb Vasc Biol, July 1, 2000; 20(7): 1737 - 1744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Roque, J. T. Fallon, J. J. Badimon, W. X. Zhang, M. B. Taubman, and E. D. Reis Mouse Model of Femoral Artery Denudation Injury Associated With the Rapid Accumulation of Adhesion Molecules on the Luminal Surface and Recruitment of Neutrophils Arterioscler Thromb Vasc Biol, February 1, 2000; 20(2): 335 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Indolfi, A. Cioppa, E. Stabile, E. Di Lorenzo, G. Esposito, A. Pisani, A. Leccia, L. Cavuto, A. M. Stingone, A. Chieffo, et al. Effects of hydroxymethylglutaryl coenzyme A reductase inhibitor simvastatin on smooth muscle cell proliferation in vitro and neointimal formation in vivo after vascular injury J. Am. Coll. Cardiol., January 1, 2000; 35(1): 214 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Natarajan, H. Pei, J.-L. Gu, J. M. Sarma, and J. Nadler Evidence for 12-lipoxygenase induction in the vessel wall following balloon injury Cardiovasc Res, February 1, 1999; 41(2): 489 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heckenkamp and G. M. Lamuraglia Intimal Hyperplasia, Arterial Remodeling, and Restenosis: An Overview Perspectives in Vascular Surgery and Endovascular Therapy, January 1, 1999; 11(2): 71 - 94. [Abstract] [PDF]
|
|
|
|
|
|
S. Kikuchi, K. Umemura, K. Kondo, A. R. Saniabadi, and M. Nakashima Photochemically Induced Endothelial Injury in the Mouse as a Screening Model for Inhibitors of Vascular Intimal Thickening Arterioscler Thromb Vasc Biol, July 1, 1998; 18(7): 1069 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Muranaka, Y. Yamasaki, Y. Nozawa, H. Terakawa, Y. Tanahashi, N. Oda, A. Satoh, T. Asao, H. Miyake, and N. Matsuura TAS-301, an Inhibitor of Smooth Muscle Cell Migration and Proliferation, Inhibits Intimal Thickening after Balloon Injury to Rat Carotid Arteries J. Pharmacol. Exp. Ther., June 1, 1998; 285(3): 1280 - 1286. [Abstract] [Full Text]
|
|
|
|
|
|
D. G. Neschis, S. D. Safford, P. N. Raghunath, D. J. Langer, M. L. David, A. K. Hanna, J. E. Tomaszewski, K. Kariko, E. S. Barnathan, and M. A. Golden Thermal Preconditioning Before Rat Arterial Balloon Injury : Limitation of Injury and Sustained Reduction of Intimal Thickening Arterioscler Thromb Vasc Biol, January 1, 1998; 18(1): 120 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hu, L. Cheng, B.-W. Hochleitner, and Q. Xu Activation of Mitogen-Activated Protein Kinases (ERK/JNK) and AP-1 Transcription Factor in Rat Carotid Arteries After Balloon Injury Arterioscler Thromb Vasc Biol, November 1, 1997; 17(11): 2808 - 2816. [Abstract] [Full Text]
|
|
|
|
|
|
R. G Statius van Eps, F. Adili, and G. M LaMuraglia Photodynamic therapy inactivates cell-associated basic fibroblast growth factor: a silent way of vascular smooth muscle cell eradication Cardiovasc Res, August 1, 1997; 35(2): 334 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Igura, S. Kawata, J.-i. Miyagawa, Y. Inui, S. Tamura, K. Fukuda, K. Isozaki, K. Yamamori, N. Taniguchi, S. Higashiyama, et al. Expression of Heparin-Binding Epidermal Growth Factor–Like Growth Factor in Neointimal Cells Induced by Balloon Injury in Rat Carotid Arteries Arterioscler Thromb Vasc Biol, December 1, 1996; 16(12): 1524 - 1531. [Abstract] [Full Text]
|
|