From the Departments of Medicine and Aging (G.D., A.M., T.A., F.C.),
Oncology and Neuroscience (A.P., S.I.), and Biomedical Sciences (T.B.),
University of Chieti "G. D'Annunzio" School of Medicine,
Chieti; Institute of Medical Pathology and Mediterranean Medicine (M.R.),
University of Messina; and the Division of Internal Medicine (P.A., G.B.B.),
Civil Hospital of Venice, Italy.
Correspondence to Giovanni Davì, MD, Cattedra di Ematologia, Università degli Studi di Chieti "G. D' Annunzio," via dei Vestini 31, 66013 Chieti, Italy. E-mail gdavi{at}unich.it
Background—Hypercholesterolemia
is considered a major risk factor for the development of
atherosclerosis. Enhanced lipid peroxidation and
persistent platelet activation can be observed in vivo in
hypercholesterolemic patients and may have
pathophysiological implications in the occurrence
of cardiovascular events. P-selectin may play an
important role in the pathogenesis of multicellular events, including
atherosclerosis. We studied the impact of
hypercholesterolemia and oxidative stress on
plasma levels of P-selectin.
Methods and Results—Plasma levels of P-selectin were measured by
means of an enzyme immunoassay in 20
hypercholesterolemic patients with no clinical evidence
of cardiovascular disease and in 20 sex- and
age-matched normocholesterolemic subjects.
Hypercholesterolemic patients had higher levels of
P-selectin compared with that of control subjects (98±61 versus 56±14
ng/mL; P=.001). They also displayed increased von
Willebrand Factor (vWF) levels (176±22 versus 119±12%;
P=.0001). A direct correlation was observed between
P-selectin and LDL cholesterol levels (
Conclusions—Hypercholesterolemia is
associated with elevated plasmatic P-selectin. Altered oxidative
processes leading to endothelial dysfunction and
persistent platelet activation may contribute to increased soluble
P-selectin levels. P-selectin may be proposed as a marker of
endothelial dysfunction in
hypercholesterolemic patients.
© 1998 American Heart Association, Inc.
Clinical Investigation and Reports
Increased Levels of Soluble P-Selectin in Hypercholesterolemic Patients
=.453).
Administration of vitamin E (600 mg/d for 2 weeks) to
hypercholesterolemic patients significantly reduced
plasma P-selectin (40%), and an inverse correlation was observed
between vitamin E and P-selectin plasma levels (=-.446).
Key Words: platelets • hypercholesterolemia • antioxidants • cell adhesion molecules
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